Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) yfjZ-ypjF/CbtA-CbeA
Location 1118558..1119225 Replicon chromosome
Accession NZ_CP079736
Organism Escherichia coli strain GB2005-delet-a-f

Toxin (Protein)


Gene name ypjF Uniprot ID Q46953
Locus tag KYX13_RS05390 Protein ID WP_001094400.1
Coordinates 1118558..1118887 (-) Length 110 a.a.

Antitoxin (Protein)


Gene name yfjZ Uniprot ID P52141
Locus tag KYX13_RS05395 Protein ID WP_000072690.1
Coordinates 1118908..1119225 (-) Length 106 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KYX13_RS05385 (1113614) 1113614..1118194 + 4581 WP_010723187.1 adhesin-like autotransporter YpjA/EhaD -
KYX13_RS05390 (1118558) 1118558..1118887 - 330 WP_001094400.1 type IV toxin-antitoxin system toxin YpjF Toxin
KYX13_RS05395 (1118908) 1118908..1119225 - 318 WP_000072690.1 type IV toxin-antitoxin system YeeU family antitoxin Antitoxin
KYX13_RS05400 (1119263) 1119263..1119463 - 201 WP_001395452.1 DUF987 domain-containing protein -
KYX13_RS05405 (1119472) 1119472..1119954 - 483 WP_001407480.1 RadC family protein -
KYX13_RS05410 (1119963) 1119963..1120421 - 459 WP_000211841.1 antirestriction protein -
KYX13_RS05415 (1120524) 1120524..1120708 - 185 Protein_1058 DUF905 domain-containing protein -
KYX13_RS05420 (1121319) 1121319..1123022 - 1704 WP_000896263.1 protein YfjW -
KYX13_RS05425 (1123164) 1123164..1124173 + 1010 Protein_1060 arsenic transporter -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Genomic island - - 1108906..1139845 30939


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(1-108)

Antitoxin

Antitoxin CbeA

(5-104)


Sequences

Toxin        


Download         Length: 110 a.a.        Molecular weight: 12308.21 Da        Isoelectric Point: 7.2761

>T209842 WP_001094400.1 NZ_CP079736:c1118887-1118558 [Escherichia coli]
MNTLPATISQAAKPCLSPVAVWQMLLTRLLEQHYGLTLNDTPFSDETVIKEHIDAGITLADAVNFLVEKYELVRIDHRGF
SWQQQSPYISVVDILRARRSTGLLKTNVK

Download         Length: 330 bp

>T209842 NZ_CP104326:c3632359-3631961 [Escherichia coli]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCCTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGAGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin


Download         Length: 106 a.a.        Molecular weight: 11737.27 Da        Isoelectric Point: 6.4630

>AT209842 WP_000072690.1 NZ_CP079736:c1119225-1118908 [Escherichia coli]
MSNTTWGLQRDITPRLGARLVQEGNQLHYLADRASITGKFSDAECPKLDVVFPHFISQIESMLTTGELNPRHAQCVTLYH
NGFTCEADTLGSCGYVYIAVYPTQR

Download         Length: 318 bp

>AT209842 NZ_CP104326:c3632655-3632362 [Escherichia coli]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCAAGTGCTGCAAGATTAGAGGAAATTACACGCCGCGCT
GAACAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A373F4I3

Antitoxin

Source ID Structure
PDB 2EA9
PDB 2JN7
AlphaFold DB P52141

References