285406

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) rnlAB/RnlA-RnlB
Location 2718299..2719736 Replicon chromosome
Accession NZ_LM993812
Organism Escherichia coli strain K-12 substr. HMS174

Toxin (Protein)


Gene name rnlA Uniprot ID P52129
Locus tag ECHMS174_RS13450 Protein ID WP_000155570.1
Coordinates 2718299..2719372 (+) Length 358 a.a.

Antitoxin (Protein)


Gene name rnlB Uniprot ID P52130
Locus tag ECHMS174_RS13455 Protein ID WP_000461704.1
Coordinates 2719365..2719736 (+) Length 124 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ECHMS174_RS13435 2713732..2715921 - 2190 WP_000136402.1 DEAD/DEAH box helicase -
ECHMS174_RS13440 2715918..2717534 - 1617 WP_000445380.1 anti-bacteriophage protein AbpA -
ECHMS174_RS13445 2717894..2718157 - 264 WP_000502050.1 DUF1524 domain-containing protein -
ECHMS174_RS13450 2718299..2719372 + 1074 WP_000155570.1 type II toxin-antitoxin system toxin mRNA endoribonuclease RnlA Toxin
ECHMS174_RS13455 2719365..2719736 + 372 WP_000461704.1 type II toxin-antitoxin system antitoxin RnlB Antitoxin
ECHMS174_RS13460 2720091..2720954 + 864 WP_000787597.1 GTPase family protein -
ECHMS174_RS13465 2721046..2721867 + 822 WP_000197391.1 DUF945 domain-containing protein -
ECHMS174_RS13470 2722084..2722785 + 702 WP_000189409.1 WYL domain-containing protein -
ECHMS174_RS13475 2722826..2723062 + 237 WP_001144029.1 hypothetical protein -
ECHMS174_RS13480 2723062..2723505 + 444 WP_000824222.1 hypothetical protein -
ECHMS174_RS13485 2723529..2723996 + 468 WP_000702495.1 hypothetical protein -
ECHMS174_RS13490 2724221..2724535 - 315 WP_000065803.1 arsenate reductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Genomic island - - 2708540..2740619 32079


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Bacterial toxin, RNase RnlA/LsoA, N-terminal repeated domain

(98-183)

Antitoxin

Antitoxin RnlB/LsoB

(14-111)


Sequences

Toxin        


Download         Length: 358 a.a.        Molecular weight: 40061.13 Da        Isoelectric Point: 6.8293

>T285406 WP_000155570.1 NZ_LM993812:2718299-2719372 [Escherichia coli]
MTIRSYKNLNLVRANIETESRQFIENKNYSIQSIGPMPGSRAGLRVVFTRPGVNLATVDIFYNGDGSTTIQYLTGANRSL
GQELADHLFETINPAEFEQVNMVLQGFVETSVLPVLELSADESHIEFREHSRNAHTVVWKIISTSYQDELTVSLHITTGK
LQIQGRPLSCYRVFTFNLAALLDLQGLEKVLIRQEDGKANIVQQEVARTYLQTVMADAYPHLHVTAEKLLVSGLCVKLAA
PDLPDYCMLLYPELRTIEGVLKSKMSGLGMPVQQPAGFGTYFDKPAAHYILKPQFAATLRPEQINIISTAYTFFNVERHS
LFHMETVVDASRMISDMARLMGKATRAWGIIKDLYIV

Download         Length: 1074 bp

Antitoxin


Download         Length: 124 a.a.        Molecular weight: 13664.69 Da        Isoelectric Point: 4.2391

>AT285406 WP_000461704.1 NZ_LM993812:2719365-2719736 [Escherichia coli]
MFEITGINVSGALKAVVMATGFENPLSSVNEIETKLSALLGSETTGEILFDLLCANGPEWNRFVTLEMKYGRIMLDTAKI
IDEQDVPTHILSKLTFTLRNHPEYLEASVLSPDDVRQVLSMDF

Download         Length: 372 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6Y2R
PDB 7AEX
PDB 6Y2Q
PDB 4I8O
PDB 6Y2P
PDB 6Y2S

Antitoxin

Source ID Structure
AlphaFold DB A0A373F4G4

References