266954

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) rnlAB/RnlA-RnlB
Location 2348591..2350028 Replicon chromosome
Accession NZ_CP114893
Organism Escherichia coli strain CM13

Toxin (Protein)


Gene name rnlA Uniprot ID P52129
Locus tag O6R07_RS11480 Protein ID WP_000155570.1
Coordinates 2348955..2350028 (-) Length 358 a.a.

Antitoxin (Protein)


Gene name rnlB Uniprot ID P52130
Locus tag O6R07_RS11475 Protein ID WP_000461704.1
Coordinates 2348591..2348962 (-) Length 124 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
O6R07_RS11440 (2343792) 2343792..2343944 + 153 Protein_2217 arsenate reductase -
O6R07_RS11445 (2344331) 2344331..2344798 - 468 WP_000702495.1 protein YfjT -
O6R07_RS11450 (2344822) 2344822..2345265 - 444 WP_000824222.1 lipoprotein YfjS -
O6R07_RS11455 (2345265) 2345265..2345501 - 237 WP_001144029.1 protein YpjK -
O6R07_RS11460 (2345542) 2345542..2346243 - 702 WP_000189409.1 WYL domain-containing protein -
O6R07_RS11465 (2346460) 2346460..2347281 - 822 WP_000197391.1 DUF932 domain-containing protein -
O6R07_RS11470 (2347373) 2347373..2348236 - 864 WP_000787597.1 GTPase family protein -
O6R07_RS11475 (2348591) 2348591..2348962 - 372 WP_000461704.1 type II toxin-antitoxin system antitoxin RnlB Antitoxin
O6R07_RS11480 (2348955) 2348955..2350028 - 1074 WP_000155570.1 type II toxin-antitoxin system toxin mRNA endoribonuclease RnlA Toxin
O6R07_RS11485 (2350170) 2350170..2350433 + 264 WP_000502050.1 DUF1524 domain-containing protein -
O6R07_RS11490 (2350793) 2350793..2352409 + 1617 WP_000445380.1 anti-bacteriophage protein AbpA -
O6R07_RS11495 (2352406) 2352406..2354595 + 2190 WP_000136402.1 DEAD/DEAH box helicase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Genomic island - - 2323387..2360392 37005


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Bacterial toxin, RNase RnlA/LsoA, N-terminal repeated domain

(98-183)

Antitoxin

Antitoxin RnlB/LsoB

(14-111)


Sequences

Toxin        


Download         Length: 358 a.a.        Molecular weight: 40061.13 Da        Isoelectric Point: 6.8293

>T266954 WP_000155570.1 NZ_CP114893:c2350028-2348955 [Escherichia coli]
MTIRSYKNLNLVRANIETESRQFIENKNYSIQSIGPMPGSRAGLRVVFTRPGVNLATVDIFYNGDGSTTIQYLTGANRSL
GQELADHLFETINPAEFEQVNMVLQGFVETSVLPVLELSADESHIEFREHSRNAHTVVWKIISTSYQDELTVSLHITTGK
LQIQGRPLSCYRVFTFNLAALLDLQGLEKVLIRQEDGKANIVQQEVARTYLQTVMADAYPHLHVTAEKLLVSGLCVKLAA
PDLPDYCMLLYPELRTIEGVLKSKMSGLGMPVQQPAGFGTYFDKPAAHYILKPQFAATLRPEQINIISTAYTFFNVERHS
LFHMETVVDASRMISDMARLMGKATRAWGIIKDLYIV

Download         Length: 1074 bp

Antitoxin


Download         Length: 124 a.a.        Molecular weight: 13664.69 Da        Isoelectric Point: 4.2391

>AT266954 WP_000461704.1 NZ_CP114893:c2348962-2348591 [Escherichia coli]
MFEITGINVSGALKAVVMATGFENPLSSVNEIETKLSALLGSETTGEILFDLLCANGPEWNRFVTLEMKYGRIMLDTAKI
IDEQDVPTHILSKLTFTLRNHPEYLEASVLSPDDVRQVLSMDF

Download         Length: 372 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6Y2R
PDB 7AEX
PDB 6Y2Q
PDB 4I8O
PDB 6Y2P
PDB 6Y2S

Antitoxin

Source ID Structure
AlphaFold DB A0A373F4G4

References