205640

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) rnlAB/RnlA-RnlB
Location 2712609..2714046 Replicon chromosome
Accession NZ_CP076404
Organism Escherichia coli strain K-12 substr. AB1157

Toxin (Protein)


Gene name rnlA Uniprot ID P52129
Locus tag KJY95_RS13200 Protein ID WP_000155570.1
Coordinates 2712609..2713682 (+) Length 358 a.a.

Antitoxin (Protein)


Gene name rnlB Uniprot ID P52130
Locus tag KJY95_RS13205 Protein ID WP_000461704.1
Coordinates 2713675..2714046 (+) Length 124 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KJY95_RS13185 (2708042) 2708042..2710231 - 2190 WP_000136402.1 DEAD/DEAH box helicase -
KJY95_RS13190 (2710228) 2710228..2711844 - 1617 WP_000445380.1 anti-bacteriophage protein AbpA -
KJY95_RS13195 (2712204) 2712204..2712467 - 264 WP_000502050.1 DUF1524 domain-containing protein -
KJY95_RS13200 (2712609) 2712609..2713682 + 1074 WP_000155570.1 type II toxin-antitoxin system toxin mRNA endoribonuclease RnlA Toxin
KJY95_RS13205 (2713675) 2713675..2714046 + 372 WP_000461704.1 type II toxin-antitoxin system antitoxin RnlB Antitoxin
KJY95_RS13210 (2714401) 2714401..2715264 + 864 WP_000787597.1 GTPase family protein -
KJY95_RS13215 (2715356) 2715356..2716177 + 822 WP_000197391.1 DUF932 domain-containing protein -
KJY95_RS13220 (2716394) 2716394..2717095 + 702 WP_000189409.1 WYL domain-containing protein -
KJY95_RS13225 (2717136) 2717136..2717372 + 237 WP_001144029.1 protein YpjK -
KJY95_RS13230 (2717372) 2717372..2717815 + 444 WP_000824222.1 lipoprotein YfjS -
KJY95_RS13235 (2717839) 2717839..2718306 + 468 WP_000702495.1 protein YfjT -
KJY95_RS13240 (2718693) 2718693..2718845 - 153 Protein_2590 arsenate reductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Genomic island - - 2702850..2744735 41885


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Bacterial toxin, RNase RnlA/LsoA, N-terminal repeated domain

(98-183)

Antitoxin

Antitoxin RnlB/LsoB

(14-111)


Sequences

Toxin        


Download         Length: 358 a.a.        Molecular weight: 40061.13 Da        Isoelectric Point: 6.8293

>T205640 WP_000155570.1 NZ_CP076404:2712609-2713682 [Escherichia coli]
MTIRSYKNLNLVRANIETESRQFIENKNYSIQSIGPMPGSRAGLRVVFTRPGVNLATVDIFYNGDGSTTIQYLTGANRSL
GQELADHLFETINPAEFEQVNMVLQGFVETSVLPVLELSADESHIEFREHSRNAHTVVWKIISTSYQDELTVSLHITTGK
LQIQGRPLSCYRVFTFNLAALLDLQGLEKVLIRQEDGKANIVQQEVARTYLQTVMADAYPHLHVTAEKLLVSGLCVKLAA
PDLPDYCMLLYPELRTIEGVLKSKMSGLGMPVQQPAGFGTYFDKPAAHYILKPQFAATLRPEQINIISTAYTFFNVERHS
LFHMETVVDASRMISDMARLMGKATRAWGIIKDLYIV

Download         Length: 1074 bp

>T205640 NZ_CP101782:110957-111106 [Klebsiella pneumoniae]
ATGACGAAATATGCCCTTATCGGGTTGCTCGCCGTGTGTGCCACGGTATTGTGTTTTTCACTGATATTCAGGGAACGGTT
ATGTGAACTGAATATTCACAGGGGAAATACAGTGGTGCAGGTAACTCTGGCCTACGAAGCACGGAAGTAA

Antitoxin


Download         Length: 124 a.a.        Molecular weight: 13664.69 Da        Isoelectric Point: 4.2391

>AT205640 WP_000461704.1 NZ_CP076404:2713675-2714046 [Escherichia coli]
MFEITGINVSGALKAVVMATGFENPLSSVNEIETKLSALLGSETTGEILFDLLCANGPEWNRFVTLEMKYGRIMLDTAKI
IDEQDVPTHILSKLTFTLRNHPEYLEASVLSPDDVRQVLSMDF

Download         Length: 372 bp

>AT205640 NZ_CP101782:c110912-110853 [Klebsiella pneumoniae]
TAAGTACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6Y2R
PDB 7AEX
PDB 6Y2Q
PDB 4I8O
PDB 6Y2P
PDB 6Y2S

Antitoxin

Source ID Structure
PDB 6Y2P
AlphaFold DB A0A373F4G4

References