Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) rnlAB/RnlA-RnlB
Location 1118270..1119707 Replicon chromosome
Accession NZ_CP099588
Organism Escherichia coli strain EMG2

Toxin (Protein)


Gene name rnlA Uniprot ID P52129
Locus tag NHF38_RS05390 Protein ID WP_000155570.1
Coordinates 1118634..1119707 (-) Length 358 a.a.

Antitoxin (Protein)


Gene name rnlB Uniprot ID P52130
Locus tag NHF38_RS05385 Protein ID WP_000461704.1
Coordinates 1118270..1118641 (-) Length 124 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NHF38_RS05350 (1113471) 1113471..1113623 + 153 Protein_1054 arsenate reductase -
NHF38_RS05355 (1114010) 1114010..1114477 - 468 WP_000702495.1 protein YfjT -
NHF38_RS05360 (1114501) 1114501..1114944 - 444 WP_000824222.1 lipoprotein YfjS -
NHF38_RS05365 (1114944) 1114944..1115180 - 237 WP_001144029.1 protein YpjK -
NHF38_RS05370 (1115221) 1115221..1115922 - 702 WP_000189409.1 WYL domain-containing protein -
NHF38_RS05375 (1116139) 1116139..1116960 - 822 WP_000197391.1 DUF932 domain-containing protein -
NHF38_RS05380 (1117052) 1117052..1117915 - 864 WP_000787597.1 GTPase family protein -
NHF38_RS05385 (1118270) 1118270..1118641 - 372 WP_000461704.1 type II toxin-antitoxin system antitoxin RnlB Antitoxin
NHF38_RS05390 (1118634) 1118634..1119707 - 1074 WP_000155570.1 type II toxin-antitoxin system toxin mRNA endoribonuclease RnlA Toxin
NHF38_RS05395 (1119849) 1119849..1120112 + 264 WP_000502050.1 DUF1524 domain-containing protein -
NHF38_RS05400 (1120472) 1120472..1122088 + 1617 WP_000445380.1 anti-bacteriophage protein AbpA -
NHF38_RS05405 (1122085) 1122085..1124274 + 2190 WP_000136402.1 DEAD/DEAH box helicase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Genomic island - - 1098191..1130071 31880


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Bacterial toxin, RNase RnlA/LsoA, N-terminal repeated domain

(98-183)

Antitoxin

Antitoxin RnlB/LsoB

(14-111)


Sequences

Toxin        


Download         Length: 358 a.a.        Molecular weight: 40061.13 Da        Isoelectric Point: 6.8293

>T249138 WP_000155570.1 NZ_CP099588:c1119707-1118634 [Escherichia coli]
MTIRSYKNLNLVRANIETESRQFIENKNYSIQSIGPMPGSRAGLRVVFTRPGVNLATVDIFYNGDGSTTIQYLTGANRSL
GQELADHLFETINPAEFEQVNMVLQGFVETSVLPVLELSADESHIEFREHSRNAHTVVWKIISTSYQDELTVSLHITTGK
LQIQGRPLSCYRVFTFNLAALLDLQGLEKVLIRQEDGKANIVQQEVARTYLQTVMADAYPHLHVTAEKLLVSGLCVKLAA
PDLPDYCMLLYPELRTIEGVLKSKMSGLGMPVQQPAGFGTYFDKPAAHYILKPQFAATLRPEQINIISTAYTFFNVERHS
LFHMETVVDASRMISDMARLMGKATRAWGIIKDLYIV

Download         Length: 1074 bp

Antitoxin


Download         Length: 124 a.a.        Molecular weight: 13664.69 Da        Isoelectric Point: 4.2391

>AT249138 WP_000461704.1 NZ_CP099588:c1118641-1118270 [Escherichia coli]
MFEITGINVSGALKAVVMATGFENPLSSVNEIETKLSALLGSETTGEILFDLLCANGPEWNRFVTLEMKYGRIMLDTAKI
IDEQDVPTHILSKLTFTLRNHPEYLEASVLSPDDVRQVLSMDF

Download         Length: 372 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6Y2R
PDB 7AEX
PDB 6Y2Q
PDB 4I8O
PDB 6Y2P
PDB 6Y2S

Antitoxin

Source ID Structure
PDB 6Y2P
AlphaFold DB A0A373F4G4

References