246440

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) rnlAB/RnlA-RnlB
Location 1117575..1119012 Replicon chromosome
Accession NZ_CP097882
Organism Escherichia coli str. K-12 substr. MG1655 strain K-12

Toxin (Protein)


Gene name rnlA Uniprot ID P52129
Locus tag NAG70_RS05395 Protein ID WP_000155570.1
Coordinates 1117939..1119012 (-) Length 358 a.a.

Antitoxin (Protein)


Gene name rnlB Uniprot ID P52130
Locus tag NAG70_RS05390 Protein ID WP_000461704.1
Coordinates 1117575..1117946 (-) Length 124 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NAG70_RS05355 (1112776) 1112776..1112928 + 153 Protein_1044 arsenate reductase -
NAG70_RS05360 (1113315) 1113315..1113782 - 468 WP_000702495.1 protein YfjT -
NAG70_RS05365 (1113806) 1113806..1114249 - 444 WP_000824222.1 lipoprotein YfjS -
NAG70_RS05370 (1114249) 1114249..1114485 - 237 WP_001144029.1 protein YpjK -
NAG70_RS05375 (1114526) 1114526..1115227 - 702 WP_000189409.1 WYL domain-containing protein -
NAG70_RS05380 (1115444) 1115444..1116265 - 822 WP_000197391.1 DUF932 domain-containing protein -
NAG70_RS05385 (1116357) 1116357..1117220 - 864 WP_000787597.1 GTPase family protein -
NAG70_RS05390 (1117575) 1117575..1117946 - 372 WP_000461704.1 type II toxin-antitoxin system antitoxin RnlB Antitoxin
NAG70_RS05395 (1117939) 1117939..1119012 - 1074 WP_000155570.1 type II toxin-antitoxin system toxin mRNA endoribonuclease RnlA Toxin
NAG70_RS05400 (1119154) 1119154..1119417 + 264 WP_000502050.1 DUF1524 domain-containing protein -
NAG70_RS05405 (1119777) 1119777..1121393 + 1617 WP_000445380.1 anti-bacteriophage protein AbpA -
NAG70_RS05410 (1121390) 1121390..1123579 + 2190 WP_000136402.1 DEAD/DEAH box helicase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Genomic island - - 1097496..1129376 31880


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Bacterial toxin, RNase RnlA/LsoA, N-terminal repeated domain

(98-183)

Antitoxin

Antitoxin RnlB/LsoB

(14-111)


Sequences

Toxin        


Download         Length: 358 a.a.        Molecular weight: 40061.13 Da        Isoelectric Point: 6.8293

>T246440 WP_000155570.1 NZ_CP097882:c1119012-1117939 [Escherichia coli str. K-12 substr. MG1655]
MTIRSYKNLNLVRANIETESRQFIENKNYSIQSIGPMPGSRAGLRVVFTRPGVNLATVDIFYNGDGSTTIQYLTGANRSL
GQELADHLFETINPAEFEQVNMVLQGFVETSVLPVLELSADESHIEFREHSRNAHTVVWKIISTSYQDELTVSLHITTGK
LQIQGRPLSCYRVFTFNLAALLDLQGLEKVLIRQEDGKANIVQQEVARTYLQTVMADAYPHLHVTAEKLLVSGLCVKLAA
PDLPDYCMLLYPELRTIEGVLKSKMSGLGMPVQQPAGFGTYFDKPAAHYILKPQFAATLRPEQINIISTAYTFFNVERHS
LFHMETVVDASRMISDMARLMGKATRAWGIIKDLYIV

Download         Length: 1074 bp

Antitoxin


Download         Length: 124 a.a.        Molecular weight: 13664.69 Da        Isoelectric Point: 4.2391

>AT246440 WP_000461704.1 NZ_CP097882:c1117946-1117575 [Escherichia coli str. K-12 substr. MG1655]
MFEITGINVSGALKAVVMATGFENPLSSVNEIETKLSALLGSETTGEILFDLLCANGPEWNRFVTLEMKYGRIMLDTAKI
IDEQDVPTHILSKLTFTLRNHPEYLEASVLSPDDVRQVLSMDF

Download         Length: 372 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6Y2R
PDB 7AEX
PDB 6Y2Q
PDB 4I8O
PDB 6Y2P
PDB 6Y2S

Antitoxin

Source ID Structure
PDB 6Y2P
AlphaFold DB A0A373F4G4

References