Detailed information of TA system TA006362

Experimentally validated

Overview

TA module

• Type: I
• Classification (family/domain): ralRA/-
• Location: 1413556..1413927
• Replicon: chromosome
• Accession: NC_000913
• Organism: Escherichia coli str. K-12 substr. MG1655
• T1TAdb ID: TA04746

Toxin (Protein)

• Gene name: ralR
• Uniprot ID: P33229
• Locus tag: b1348
• Protein ID: NP_415864.1
• Coordinates: 1413733..1413927 (-)
• Length: 65 a.a.

Antitoxin (RNA)

Gene name	ralA
Locus tag	b4714
Coordinates	1413556..1413734 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
b4743	1409308..1409481	+	174	YP_009518775.1	protein YnaL	-
b1343	1409511..1410884	+	1374	NP_415859.1	ATP-dependent RNA helicase DbpA	-
b1344	1411013..1411948	-	936	NP_415860.1	tRNA cytosine(32) 2-sulfurtransferase TtcA	-
b1345	1412000..1413235	-	1236	NP_415861.1	putative integrase	-
b1346	1413237..1413452	-	216	NP_415862.4	excisionase	-
b1347	1413531..1413740	-	210	NP_415863.1	double-strand break reduction protein	-
-	1413556..1413734	+	179	-	-	Antitoxin
b1348	1413733..1413927	-	195	NP_415864.1	endodeoxyribonuclease toxin RalR	Toxin
b1349	1413984..1414793	-	810	NP_415865.1	recombinase RecT	-
b1350	1414786..1417386	-	2601	NP_415866.1	exonuclease VIII, ds DNA exonuclease, 5' --> 3' specific	-
b1351	1417488..1417763	-	276	NP_415867.1	protein RacC	-
b4526	1417838..1418008	-	171	YP_588451.1	zinc-binding protein	-
b1352	1418008..1418229	-	222	NP_415869.2	inhibitor of FtsZ	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	Similar MGE(s) Similar MGE(s) is defined as other TA-related MGE(s) with Mash distance < 0.01 to this MGE. Detail Genome accession Organism MGE type Related TA Coordinates Mash distance View CP009789 Escherichia coli K-12 strain ER3413 Prophage TA020020 1385172..1414104 0.008 View HG738867 Escherichia coli str. K-12 substr. MC4100 Prophage TA020061 1300529..1322203 0 View NC_012759 Escherichia coli BW2952 Prophage TA023848 1302083..1323757 0 View NC_017638 Escherichia coli DH1 Prophage TA027398 1394182..1415856 0 View NZ_CP009273 Escherichia coli BW25113 Prophage TA048677 1406257..1427931 0 View HG738867 Escherichia coli str. K-12 substr. MC4100 Prophage TA020061 1300529..1322203 0 View NC_010473 Escherichia coli str. K-12 substr. DH10B Prophage TA022680 1499420..1521094 0 View NC_007779 Escherichia coli str. K-12 substr. W3110 Prophage TA021432 1413714..1435388 0 View CP009789 Escherichia coli K-12 strain ER3413 Prophage TA020020 1385172..1414104 0.008 View NZ_HG738867 Escherichia coli str. K-12 substr. MC4100 strain K-12 Prophage TA285012 1300529..1322203 0 View NZ_CP025520 Escherichia coli strain DH5alpha Prophage TA090473 269442..291116 0 View NZ_CP008801 Escherichia coli KLY Prophage TA047497 1387282..1408956 0 View NZ_CP009644 Escherichia coli ER2796 strain K-12 ER2796 Prophage TA049358 1385171..1414103 0.008 View NZ_CP009789 Escherichia coli K-12 strain K-12 ER3413 Prophage TA049785 1385172..1414104 0.008 View NZ_CP010438 Escherichia coli K-12 strain K-12 MG1655 Prophage TA052430 2167667..2189340 0 View NZ_CP010439 Escherichia coli K-12 strain K-12 MG1655 Prophage TA052463 2145223..2174155 0.008 View NZ_CP010440 Escherichia coli K-12 strain K-12 MG1655 Prophage TA052502 2169121..2190794 0 View NZ_CP010441 Escherichia coli K-12 strain K-12 MG1655 Prophage TA052542 2155573..2177246 0 View NZ_CP010442 Escherichia coli K-12 strain K-12 MG1655 Prophage TA052584 2208369..2230043 0 View NZ_CP010444 Escherichia coli K-12 strain K-12 MG1655 Prophage TA052666 2157567..2179241 0 View NZ_CP010445 Escherichia coli K-12 strain K-12 MG1655 Prophage TA052708 2208368..2230042 0 View NZ_CP011320 Escherichia coli strain SQ37 Prophage TA053838 1410025..1431699 0 View NZ_CP011321 Escherichia coli strain SQ88 Prophage TA053881 1404487..1426161 0 View NZ_CP011322 Escherichia coli strain SQ110 Prophage TA053924 1404486..1426160 0 View NZ_CP011324 Escherichia coli strain SQ2203 Prophage TA053967 1404487..1426161 0 View NZ_CP011342 Escherichia coli K-12 substr. GM4792 Prophage TA054054 1393868..1415542 0 View NZ_CP011343 Escherichia coli K-12 substr. GM4792 Prophage TA054093 1385669..1414602 0.008 View NZ_CP011495 Escherichia coli strain NCM3722 isolate K-12 Prophage TA054319 2664138..2685812 0 View NZ_CP012125 Escherichia coli strain DH1Ec095 Prophage TA055834 1394183..1415857 0 View NZ_CP012126 Escherichia coli strain DH1Ec104 Prophage TA055877 1394184..1415858 0 View NZ_CP012127 Escherichia coli strain DH1Ec169 Prophage TA055920 1391928..1413602 0 View NZ_CP014225 Escherichia coli str. K-12 substr. MG1655 strain K-12 Prophage TA060408 4250924..4272598 0 View NZ_CP014270 Escherichia coli K-12 strain K-12 DHB4 Prophage TA060521 1317105..1338779 0 View NZ_CP014272 Escherichia coli K-12 strain K-12 C3026 Prophage TA060573 1515182..1537843 0.001 View NZ_CP017100 Escherichia coli strain K-12 NEB 5-alpha Prophage TA065972 1357884..1379558 0 View NZ_CP019629 Escherichia coli strain MGY Prophage TA073070 1412000..1433674 0 View NZ_CP025268 Escherichia coli str. K-12 substr. MG1655 strain K-12 Prophage TA089990 1416819..1438493 0.001 View NZ_CP026026 Escherichia coli strain CAR Prophage TA092756 1410024..1431698 0 View NZ_CP026027 Escherichia coli strain LIM Prophage TA092797 1410025..1431699 0 View NZ_CP026028 Escherichia coli strain CIT Prophage TA092840 1410024..1431698 0 View NZ_CP026342 Escherichia coli strain 9FA Prophage TA093855 1412000..1433674 0 View NZ_CP026344 Escherichia coli strain 8FA Prophage TA093941 1412000..1433674 0 View NZ_CP026345 Escherichia coli strain 8A Prophage TA093984 1412000..1433674 0 View NZ_CP026346 Escherichia coli strain 7FA Prophage TA094027 1412000..1433674 0 View NZ_CP026347 Escherichia coli strain 7A Prophage TA094070 1412000..1433674 0 View NZ_CP026348 Escherichia coli strain 6FA Prophage TA094113 1412000..1433674 0 View NZ_CP026349 Escherichia coli strain 6A Prophage TA094156 1412000..1433674 0 View NZ_CP026350 Escherichia coli strain 5FA Prophage TA094199 1412000..1433674 0 View NZ_CP026351 Escherichia coli strain 5A Prophage TA094242 1412000..1433674 0 View NZ_CP026352 Escherichia coli strain 4FA Prophage TA094285 1412000..1433674 0 View NZ_CP026354 Escherichia coli strain 3FA Prophage TA094371 1412000..1433674 0 View NZ_CP026355 Escherichia coli strain 3A Prophage TA094414 1412000..1433674 0 View NZ_CP026356 Escherichia coli strain 2FA Prophage TA094457 1412000..1433674 0 View NZ_CP026357 Escherichia coli strain 2F_0 Prophage TA094500 1412000..1433674 0 View NZ_CP026358 Escherichia coli strain 2A Prophage TA094543 1412000..1433674 0 View NZ_CP026359 Escherichia coli strain 2_0 Prophage TA094586 1412000..1433674 0 View NZ_CP026360 Escherichia coli strain 1FA Prophage TA094629 1412000..1433674 0 View NZ_CP026361 Escherichia coli strain 1A Prophage TA094672 1412000..1433674 0 View NZ_CP028702 Escherichia coli strain J53 Prophage TA102952 1845328..1867002 0 View NZ_CP028703 Escherichia coli strain ME8067 Prophage TA102996 2270208..2291882 0 View NZ_CP029238 Escherichia coli strain CJ236 Prophage TA105244 1412000..1433674 0 View NZ_CP030240 Escherichia coli strain ER1709 Prophage TA107385 3540935..3562607 0 View NZ_CP032679 Escherichia coli str. K-12 substr. MG1655 strain K-12 Prophage TA112101 1412005..1433679 0 View NZ_CP034658 Escherichia coli strain ATCC 98082 Prophage TA116308 1378571..1400245 0 View NZ_CP037857 Escherichia coli BW25113 strain AM187 Prophage TA120207 1405919..1427593 0 View NZ_CP040643 Escherichia coli strain BE104 Prophage TA126259 1545964..1567638 0 View NZ_CP040663 Escherichia coli strain EK2009 Prophage TA126346 1400917..1422591 0.002 View NZ_CP040664 Escherichia coli strain KR2009 Prophage TA126389 1399878..1421551 0.002 View NZ_CP042843 Escherichia coli strain JME67 Prophage TA136085 2121489..2143163 0 View NZ_CP042845 Escherichia coli strain JME65 Prophage TA136171 2116376..2138050 0 View NZ_CP042846 Escherichia coli strain JME64 Prophage TA136214 2111495..2133169 0 View NZ_CP043185 Escherichia coli O16:H48 strain PG20180171 Prophage TA137422 140259..161933 0 View NZ_CP043199 Escherichia coli O16:H48 strain PG20180060 Prophage TA137666 2443910..2465584 0 View NZ_CP044410 Escherichia coli strain ecMN1F Prophage TA140887 2110299..2139231 0.008 View NZ_CP053603 Escherichia coli strain Dam/Dcm Prophage TA157059 1396047..1417720 0 View NZ_CP053604 Escherichia coli strain NEB10-beta Prophage TA157096 1502039..1523712 0 View NZ_CP053605 Escherichia coli strain NEB_Turbo Prophage TA157126 1298363..1320037 0 View NZ_CP053607 Escherichia coli strain NEB5-alpha_F'Iq Prophage TA157171 1358898..1380572 0 View NZ_CP058948 Escherichia coli strain SY3626C5 Prophage TA167997 1845036..1866710 0 View NZ_CP059043 Escherichia coli strain SY3626C1 Prophage TA168125 1845036..1866710 0 View NZ_CP060708 Escherichia coli strain MFDpir Prophage TA170774 1396450..1418124 0 View NZ_CP062239 Escherichia coli strain AML001_ev03 Prophage TA173640 1406257..1427931 0 View NZ_CP062240 Escherichia coli strain AML001_ev02 Prophage TA173683 1406257..1427931 0 View NZ_CP062241 Escherichia coli strain AML001_ev01 Prophage TA173726 1406257..1427931 0 View NZ_CP062242 Escherichia coli strain AML001_par Prophage TA173769 1406257..1427931 0 View NZ_CP062243 Escherichia coli strain AML003_ev03 Prophage TA173812 1406257..1427931 0 View NZ_CP062244 Escherichia coli strain AML003_ev02 Prophage TA173855 1406257..1427931 0 View NZ_CP062245 Escherichia coli strain AML003_ev01 Prophage TA173898 1406256..1427930 0 View NZ_CP062246 Escherichia coli strain AML003_par Prophage TA173941 1406257..1427931 0 View NZ_CP062247 Escherichia coli strain AML002_ev03 Prophage TA173984 1407460..1429134 0 View NZ_CP062248 Escherichia coli strain AML002_ev02 Prophage TA174027 1406257..1427931 0 View NZ_CP062249 Escherichia coli strain AML002_ev01 Prophage TA174070 1407456..1429130 0 View NZ_CP062250 Escherichia coli strain AML002_par Prophage TA174113 1406257..1427931 0 View NZ_CP064678 Escherichia coli K-12 strain CETR3G40 Prophage TA181421 1406257..1427931 0 View NZ_CP064679 Escherichia coli K-12 strain CETR2G40 Prophage TA181464 1406257..1427931 0 View NZ_CP064680 Escherichia coli K-12 strain CETR1G40 Prophage TA181507 1406257..1427931 0 View NZ_CP064681 Escherichia coli K-12 strain BZKR3G40 Prophage TA181550 1406257..1427931 0 View NZ_CP064682 Escherichia coli K-12 strain BZKR2G40 Prophage TA181593 1406257..1427931 0 View NZ_CP068281 Escherichia coli strain CY706 Prophage TA187052 2654102..2675776 0 View NZ_CP069132 Escherichia coli BW25113 substr. CHXR1G20 Prophage TA189913 1406253..1427927 0 View NZ_CP069133 Escherichia coli BW25113 substr. CHXR2G20 Prophage TA189956 1406257..1427931 0 View NZ_CP069386 Escherichia coli strain XH1815 Prophage TA190289 35060..63993 0.008 View NZ_CP072054 Escherichia coli strain K1508 Prophage TA197253 1300529..1322203 0 View NZ_CP077969 Escherichia coli strain JM83 Prophage TA208751 1329478..1351152 0 View NZ_CP083410 Escherichia coli strain RP437 Prophage TA216365 1411998..1433672 0 View NZ_CP084893 Escherichia coli strain g94 Prophage TA219821 1405619..1434552 0.008 View NZ_CP084895 Escherichia coli strain g74 Prophage TA219907 1405619..1434552 0.008 View NZ_CP084897 Escherichia coli strain gb37 Prophage TA219993 1405619..1434552 0.008 View NZ_CP084898 Escherichia coli strain gw25 Prophage TA220036 1405619..1434552 0.008 View NZ_CP084899 Escherichia coli strain W3110-P Prophage TA220079 1405619..1434552 0.008 View NZ_CP092037 Escherichia coli strain MG1655-377GD Prophage TA235505 1293781..1315455 0 View NZ_CP092702 Escherichia coli strain S-P-C-029.01 Prophage TA236974 1412000..1433674 0 View NZ_CP102378 Escherichia coli strain JB41 Prophage TA253501 1415377..1444310 0.008 View NZ_CP102379 Escherichia coli strain BM28 lysU Prophage TA253536 1284440..1306114 0 View NZ_CP102380 Escherichia coli strain BM28 Prophage TA253570 1284440..1306114 0 View NZ_CP110014 Escherichia coli strain HE_100-10 Prophage TA262636 1499409..1521083 0 View NZ_CP110015 Escherichia coli strain HE_150 Prophage TA262662 1499412..1521085 0.001 View NZ_CP110016 Escherichia coli strain HE_100 Prophage TA262688 1499413..1521087 0 View NZ_CP110017 Escherichia coli strain HE_50 Prophage TA262714 1499412..1521086 0 View NZ_CP110018 Escherichia coli strain DH10B Prophage TA262740 1499420..1521094 0 View NZ_CP114892 Escherichia coli strain CM5 Prophage TA266915 629780..651454 0 View NZ_CP114894 Escherichia coli strain CM19 Prophage TA266964 756051..784984 0.008 View NZ_CP114895 Escherichia coli strain CM56 Prophage TA266997 2052081..2073755 0 View NZ_CP114896 Escherichia coli strain CM93 Prophage TA267016 867311..888985 0 View NZ_CP122315 Escherichia coli strain HT-947 Prophage TA276642 2816191..2845124 0.008 View NZ_CP122317 Escherichia coli strain HT-948 Prophage TA276717 4549295..4570969 0 View NZ_CP122319 Escherichia coli strain BW25113 Prophage TA276772 3847851..3869525 0 View NZ_CP128218 Escherichia coli strain TG1 Prophage TA284412 1034367..1056041 0 View NZ_LM995446 Escherichia coli strain K-12 substr. RV308 Prophage TA285430 1360897..1382571 0 View NZ_LR881938 Escherichia coli str. K-12 substr. MG1655 strain K-12 Prophage TA289509 1396269..1417943 0 View NC_007779 Escherichia coli str. K-12 substr. W3110 Prophage TA021432 1413714..1435388 0 View NC_010473 Escherichia coli str. K-12 substr. DH10B Prophage TA022680 1499420..1521094 0 View NC_012759 Escherichia coli BW2952 Prophage TA023848 1302083..1323757 0 View NC_017638 Escherichia coli DH1 Prophage TA027398 1394182..1415856 0 View NZ_CP009273 Escherichia coli BW25113 Prophage TA048677 1406257..1427931 0 View NZ_CP025520 Escherichia coli strain DH5alpha Prophage TA090473 269442..291116 0 View NZ_HG738867 Escherichia coli str. K-12 substr. MC4100 strain K-12 Prophage TA285012 1300529..1322203 0	inside	Prophage	-	-	1412000..1433674	21674

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Endodeoxyribonuclease toxin RalR (6-54)

Sequences

Toxin

Protein

• Length: 65 a.a.
• Molecular weight: 7018.89 Da
• Isoelectric Point: 8.9538

>T6362 NP_415864.1 NC_000913:c1413927-1413733 [Escherichia coli str. K-12 substr. MG1655]
MRYDNVKPCPFCGCPSVTVKAISGYYRAKCNGCESRTGYGGSEKEALERWNKRTTGNNNGGVHV

Nucleotide

• Length: 195 bp

>T6362 NC_000913:c1413927-1413733 [Escherichia coli str. K-12 substr. MG1655]
ATGAGATATGACAATGTTAAACCATGTCCATTTTGTGGTTGTCCATCAGTAACGGTGAAAGCCATTTCAGGATATTACCG
AGCGAAGTGTAACGGATGCGAATCCCGAACCGGTTATGGTGGAAGTGAAAAAGAAGCACTCGAAAGATGGAATAAACGAA
CCACTGGAAATAATAATGGAGGTGTTCATGTATAA

Antitoxin

Nucleotide

• Length: 179 bp

>AT6362 NC_000913:1413556-1413734 [Escherichia coli str. K-12 substr. MG1655]
GAGGACTGAAGTTTCTCGCAATTAAAATTTATCAGTTTTACTTTCTGCTCTCTGGAAACGCCTGCTTCTTTTTTACCTGA
GAGCATTTTTTCGCATTCTGATTTCGTTAGTTTAGATTTTGAATATCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTT
CAATAGTGGCGGTAATTTT

Similar Proteins

Only experimentally validated proteins are listed.

Toxin

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
T10218	Escherichia coli O157:H7 str. EDL933	100	100	1

Multiple sequence alignment

Structures

Toxin

• AlphaFold DB: P33229

Antitoxin

References

(1) Yunxue Guo et al. (2014) RalR (a DNase) and RalA (a small RNA) form a type I toxin-antitoxin system in Escherichia coli. Nucleic Acids Research 42(10):6448-62. [PubMed:24748661]


 experimental literature