Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type I Classification (family/domain) ralRA/-
Location 1413556..1413927 Replicon chromosome
Accession NZ_CP092702
Organism Escherichia coli strain S-P-C-029.01

Toxin (Protein)


Gene name ralR Uniprot ID F4VC37
Locus tag MJ592_RS06985 Protein ID WP_001317028.1
Coordinates 1413733..1413927 (-) Length 65 a.a.

Antitoxin (RNA)


Gene name ralA
Locus tag -
Coordinates 1413556..1413734 (+)

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MJ592_RS22570 (1409308) 1409308..1409481 + 174 WP_001296046.1 protein YnaL -
MJ592_RS06960 (1409511) 1409511..1410884 + 1374 WP_000123737.1 ATP-dependent RNA helicase DbpA -
MJ592_RS06965 (1411013) 1411013..1411948 - 936 WP_001157406.1 tRNA 2-thiocytidine(32) synthetase TtcA -
MJ592_RS06970 (1412000) 1412000..1413235 - 1236 WP_000040852.1 site-specific integrase -
MJ592_RS06975 (1413237) 1413237..1413452 - 216 WP_000079604.1 excisionase XisR -
- (1413556) 1413556..1413734 + 179 NuclAT_0 - Antitoxin
- (1413556) 1413556..1413734 + 179 NuclAT_0 - Antitoxin
- (1413556) 1413556..1413734 + 179 NuclAT_0 - Antitoxin
- (1413556) 1413556..1413734 + 179 NuclAT_0 - Antitoxin
MJ592_RS06980 (1413531) 1413531..1413740 - 210 WP_000276809.1 double-strand break reduction protein RcbA -
MJ592_RS06985 (1413733) 1413733..1413927 - 195 WP_001317028.1 type I toxin-antitoxin system endodeoxyribonuclease toxin RalR Toxin
MJ592_RS06990 (1413984) 1413984..1414793 - 810 WP_000166319.1 recombination protein RecT -
MJ592_RS06995 (1414786) 1414786..1417386 - 2601 WP_000105143.1 exodeoxyribonuclease VIII -
MJ592_RS07000 (1417488) 1417488..1417763 - 276 WP_000632297.1 protein RacC -
MJ592_RS07005 (1417838) 1417838..1418008 - 171 WP_001352098.1 YdaE family protein -
MJ592_RS07010 (1418008) 1418008..1418229 - 222 WP_000560225.1 killing protein KilR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Prophage - - 1412000..1433674 21674


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Endodeoxyribonuclease toxin RalR

(6-54)


Sequences

Toxin        


Download         Length: 65 a.a.        Molecular weight: 7018.89 Da        Isoelectric Point: 8.9538

>T236974 WP_001317028.1 NZ_CP092702:c1413927-1413733 [Escherichia coli]
MRYDNVKPCPFCGCPSVTVKAISGYYRAKCNGCESRTGYGGSEKEALERWNKRTTGNNNGGVHV

Download         Length: 195 bp

>T236974 NZ_LR778142:4554133-4554507 [Escherichia coli]
ATGGCAAAAAGAACTGCGCTTTTTGATACCAATATCCTTATCGATTTGTTCAGCGGACGAATTGAGGCAAAACAGGCTCT
GGAGGCATGGCCCCTACAGAATGCGATTAGTCTGGTCACATGGATGGAGGTGCTGGTTGGCGCAAAAAAATATGATCAAG
AACATCGCACACGGATTGCAATGAGTGCGTTTAACGTTATTGGGATTTCGCAGGATATCGCGGAACGAAGCGTTGCTTTA
AGGCAGGAGTATAGAATGAAACTTCCAGATGCCATTATTCTGGCAACAGCACAGATTTATGGCTTTGAACTGGTAACGCG
AAATACGAGAGATTTTGCGGGAATAGCAGGTGTCGTCACACCCTATGAGCTGTAA

Antitoxin


Download         Length: 179 bp

>AT236974 NZ_CP092702:1413556-1413734 [Escherichia coli]
GAGGACTGAAGTTTCTCGCAATTAAAATTTATCAGTTTTACTTTCTGCTCTCTGGAAACGCCTGCTTCTTTTTTACCTGA
GAGCATTTTTTCGCATTCTGATTTCGTTAGTTTAGATTTTGAATATCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTT
CAATAGTGGCGGTAATTTT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7L6LHV7

Antitoxin

Download structure file

References