Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type I Classification (family/domain) ralRA/-
Location 1302085..1302456 Replicon chromosome
Accession NZ_CP072054
Organism Escherichia coli strain K1508

Toxin (Protein)


Gene name ralR Uniprot ID F4VC37
Locus tag J5N95_RS06355 Protein ID WP_001317028.1
Coordinates 1302262..1302456 (-) Length 65 a.a.

Antitoxin (RNA)


Gene name ralA
Locus tag -
Coordinates 1302085..1302263 (+)

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
J5N95_RS22025 (1297837) 1297837..1298010 + 174 WP_001296046.1 protein YnaL -
J5N95_RS06330 (1298040) 1298040..1299413 + 1374 WP_000123737.1 ATP-dependent RNA helicase DbpA -
J5N95_RS06335 (1299542) 1299542..1300477 - 936 WP_001157406.1 tRNA 2-thiocytidine(32) synthetase TtcA -
J5N95_RS06340 (1300529) 1300529..1301764 - 1236 WP_000040852.1 site-specific integrase -
J5N95_RS06345 (1301766) 1301766..1301981 - 216 WP_000079604.1 excisionase XisR -
- (1302085) 1302085..1302263 + 179 NuclAT_0 - Antitoxin
- (1302085) 1302085..1302263 + 179 NuclAT_0 - Antitoxin
- (1302085) 1302085..1302263 + 179 NuclAT_0 - Antitoxin
- (1302085) 1302085..1302263 + 179 NuclAT_0 - Antitoxin
J5N95_RS06350 (1302060) 1302060..1302269 - 210 WP_000276809.1 double-strand break reduction protein RcbA -
J5N95_RS06355 (1302262) 1302262..1302456 - 195 WP_001317028.1 type I toxin-antitoxin system endodeoxyribonuclease toxin RalR Toxin
J5N95_RS06360 (1302513) 1302513..1303322 - 810 WP_000166319.1 recombination protein RecT -
J5N95_RS06365 (1303315) 1303315..1305915 - 2601 WP_000105143.1 exodeoxyribonuclease VIII -
J5N95_RS06370 (1306017) 1306017..1306292 - 276 WP_000632297.1 protein RacC -
J5N95_RS06375 (1306367) 1306367..1306537 - 171 WP_001352098.1 YdaE family protein -
J5N95_RS06380 (1306537) 1306537..1306758 - 222 WP_000560225.1 killing protein KilR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Prophage - - 1300529..1322203 21674


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Endodeoxyribonuclease toxin RalR

(6-54)


Sequences

Toxin        


Download         Length: 65 a.a.        Molecular weight: 7018.89 Da        Isoelectric Point: 8.9538

>T197253 WP_001317028.1 NZ_CP072054:c1302456-1302262 [Escherichia coli]
MRYDNVKPCPFCGCPSVTVKAISGYYRAKCNGCESRTGYGGSEKEALERWNKRTTGNNNGGVHV

Download         Length: 195 bp

>T197253 NZ_CP095851:c839577-839008 [Rhodococcus pyridinivorans]
ATGAGGCGTCTGGATCTGATCGTCGGTCCCAACGGTTCCGGCAAGTCGACGTTCGTCGAGTTGTCCCTCGCTCCCCTGCT
GCCGCGCAGCGTGTTCGTCAATGCGGATGTGATCGCGCGGCAGCGCTGGCCCGACCGGGCGGAGGAGATGTCGTACGAGG
CGGCGCAGCTCGCCGCGGCGGCGCGGGACCGGTTGCTGCGGCAGGGCCGGTCGTTCATTGCGGAGACGGTGTTCTCGCAT
CCGTCGAAGCTGGAGTTGATCGACCGCGCGCACGAGGTGGGGTTCACGGTGGTGCTGCACGTGATGCTGGTGCCCGAGGC
GATGTCGGTGCTGCGTGTACAGCACCGGGTGGCCTCCGGTGGGCATGCGGTGCCCGAGGACAAGATCCGGGCGCGTTATC
GGCGGTTGTGGCCGCTGGTGACCGAAGCGATCGGCCGCGCCGATCAGGCGTCGGTGTACGACACGGCGTCCGGGACCCCG
GTGGTGGTGGCGCGGTTCGCCGGCGGGGTGCCGGTGGGTGCGGTGCGCTGGCCGGGATGGGCGCCGGCGGCGCTCACCGG
GCTCGGCTGA

Antitoxin


Download         Length: 179 bp

>AT197253 NZ_CP072054:1302085-1302263 [Escherichia coli]
GAGGACTGAAGTTTCTCGCAATTAAAATTTATCAGTTTTACTTTCTGCTCTCTGGAAACGCCTGCTTCTTTTTTACCTGA
GAGCATTTTTTCGCATTCTGATTTCGTTAGTTTAGATTTTGAATATCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTT
CAATAGTGGCGGTAATTTT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7L6LHV7

Antitoxin

Download structure file

References