Detailed information of TA system TA174070

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): ralRA/-
• Location: 1409012..1409383
• Replicon: chromosome
• Accession: NZ_CP062249
• Organism: Escherichia coli strain AML002_ev01

Toxin (Protein)

• Gene name: ralR
• Uniprot ID: F4VC37
• Locus tag: IHN46_RS06925
• Protein ID: WP_001317028.1
• Coordinates: 1409189..1409383 (-)
• Length: 65 a.a.

Antitoxin (RNA)

Gene name	ralA
Locus tag	-
Coordinates	1409012..1409190 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IHN46_RS06900	1404967..1406340	+	1374	WP_000123737.1	ATP-dependent RNA helicase DbpA	-
IHN46_RS06905	1406469..1407404	-	936	WP_001157406.1	tRNA 2-thiocytidine(32) synthetase TtcA	-
IHN46_RS06910	1407456..1408691	-	1236	WP_000040852.1	site-specific integrase	-
IHN46_RS06915	1408693..1408908	-	216	WP_000079604.1	excisionase XisR	-
IHN46_RS06920	1408987..1409196	-	210	WP_000276809.1	double-strand break reduction protein RcbA	-
-	1409012..1409190	+	179	NuclAT_0	-	Antitoxin
-	1409012..1409190	+	179	NuclAT_0	-	Antitoxin
-	1409012..1409190	+	179	NuclAT_0	-	Antitoxin
-	1409012..1409190	+	179	NuclAT_0	-	Antitoxin
IHN46_RS06925	1409189..1409383	-	195	WP_001317028.1	type I toxin-antitoxin system endodeoxyribonuclease toxin RalR	Toxin
IHN46_RS06930	1409440..1410249	-	810	WP_000166319.1	recombination protein RecT	-
IHN46_RS06935	1410242..1412842	-	2601	WP_000105143.1	exodeoxyribonuclease VIII	-
IHN46_RS06940	1412944..1413219	-	276	WP_000632297.1	protein RacC	-
IHN46_RS06945	1413294..1413464	-	171	WP_001352098.1	conserved protein, Rac prophage	-
IHN46_RS06950	1413464..1413685	-	222	WP_000560225.1	killing protein KilR	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	Similar MGE(s) Similar MGE(s) is defined as other TA-related MGE(s) with Mash distance < 0.01 to this MGE. Detail Genome accession Organism MGE type Related TA Coordinates Mash distance View CP009789 Escherichia coli K-12 strain ER3413 Prophage TA020020 1385172..1414104 0.008 View HG738867 Escherichia coli str. K-12 substr. MC4100 Prophage TA020061 1300529..1322203 0 View NC_000913 Escherichia coli str. K-12 substr. MG1655 Prophage TA006362 1412000..1433674 0 View NC_007779 Escherichia coli str. K-12 substr. W3110 Prophage TA021432 1413714..1435388 0 View NC_010473 Escherichia coli str. K-12 substr. DH10B Prophage TA022680 1499420..1521094 0 View NC_012759 Escherichia coli BW2952 Prophage TA023848 1302083..1323757 0 View NC_017638 Escherichia coli DH1 Prophage TA027398 1394182..1415856 0 View NZ_CP009273 Escherichia coli BW25113 Prophage TA048677 1406257..1427931 0 View NZ_CP025520 Escherichia coli strain DH5alpha Prophage TA090473 269442..291116 0 View NZ_HG738867 Escherichia coli str. K-12 substr. MC4100 strain K-12 Prophage TA285012 1300529..1322203 0	inside	Prophage	-	-	1407456..1429130	21674

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Endodeoxyribonuclease toxin RalR (6-54)

Sequences

Toxin

Protein

• Length: 65 a.a.
• Molecular weight: 7018.89 Da
• Isoelectric Point: 8.9538

>T174070 WP_001317028.1 NZ_CP062249:c1409383-1409189 [Escherichia coli]
MRYDNVKPCPFCGCPSVTVKAISGYYRAKCNGCESRTGYGGSEKEALERWNKRTTGNNNGGVHV

Nucleotide

• Length: 195 bp

>T174070 NZ_CP080528:386366-387619 [Mesorhizobium sp. AR07]
ATGACTCATCTACTGGACGTATATTTCAGGGAGACCAAGGCCGGCATTCTCAGCCAGGACGAGGACGGCGCGCTGGGCTA
CGCCTATTACGCCGACTATCTCGCCGGGCAGCAGCCCCGCGTCATTTCCTTCTCGATGCCGCTGCGGGAAGAACCCTATG
CCGACCGGGTCGCGCGTCCGTTCTTCTCGGGCCTTCTTCCGGACGAGGGCGCGCGGCAGCGTCTTGCCGGAGCGCTTGGC
CTTTCGGCTGGCAATGCGTTCGGCCTGCTCAAGGTCATCGGCGGCGAATGCGCGGGCGCCTTGTCGTTGTATCCAGCTGG
TGAAGCGCCGGCTCCTTCCGACGATGACGAGGCTCTCAGTGCAGAGCGACTCAAGGAAATCATCGGCAAGCTGCGGGAGC
GGCCCCTGCTCGGGGGAGAGGAGGGCGTTCGTCTCTCACTGGCCGGCGCCCAGGACAAGCTCGCGGTGATCGTCGAAGGC
GAGACGATCGGGCTAGCCAAGGGCGGCCGCCCAACCACCCATATTCTGAAGCCGGTCATCCAGGCGCTGGAAGGAACCGT
CGAGAACGAGCTCTTCTGTCTGCGCCTGGCAGAGCGTCTCCAACTGCCGGTTCCAAGGGTCGAGATGCGCCGTGCCGGCG
ACACCGCGTTTCTTCTGATCCAGCGCTATGACCGTGCGCGGACTGACGGCGGACACATCGAACGATTGCACCAGGAGGAT
TTCTGCCAGGCGCTCAGCGTTCCACCCGAACTCAAATATGAAGAAGAGGGCGGTCCCGGTACCAAGAGCTCGCTGGACCT
GATCGATCGGGCCTGCGCACGACCGGCGGCGGACCGGTTGCGCTTCATCCGGATGCTGATCTTTCATTATCTCCTGGGCA
ATGCCGATGCGCACGGAAAGAATTACGCGCTGCTCTATGACGGCGATACGCCGAATCTCGCCCCACTCTACGACGTGGTT
TGCACCGCCGCCTATCCGCGGCTGGCCAAGAAGCTCGCCATGGCGATCGGCGGACGATCCGTTCCCGACACGATCCAACT
GAAGCACTGGCTGACGCTCGTTCCCGAAACCCGGGGCGCGCAACGCCTTCTCATCCGCGATATCGCAACCATGGCCGGGC
GCATTGGAGATGAGGCCGACGCTTTGCTCGCGCAGCTGGCGAATGTAGGGGCGGACCACGCCATTCTAAAATCCGTGCGC
GCAGTCATCGCGACCCGGGCAACGCACCTTTTGCGGATGACCGAAGGTGGATGA

Antitoxin

Nucleotide

• Length: 179 bp

>AT174070 NZ_CP062249:1409012-1409190 [Escherichia coli]
GAGGACTGAAGTTTCTCGCAATTAAAATTTATCAGTTTTACTTTCTGCTCTCTGGAAACGCCTGCTTCTTTTTTACCTGA
GAGCATTTTTTCGCATTCTGATTTCGTTAGTTTAGATTTTGAATATCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTT
CAATAGTGGCGGTAATTTT

Structures

Toxin

• AlphaFold DB: A0A7L6LHV7

Antitoxin