Detailed information of TA system TA219993

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): ralRA/-
• Location: 1414434..1414805
• Replicon: chromosome
• Accession: NZ_CP084897
• Organism: Escherichia coli strain gb37

Toxin (Protein)

• Gene name: ralR
• Uniprot ID: F4VC37
• Locus tag: LHV64_RS06985
• Protein ID: WP_001317028.1
• Coordinates: 1414611..1414805 (-)
• Length: 65 a.a.

Antitoxin (RNA)

Gene name	ralA
Locus tag	-
Coordinates	1414434..1414612 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LHV64_RS06960 (1410389)	1410389..1411762	+	1374	WP_000123737.1	ATP-dependent RNA helicase DbpA	-
LHV64_RS06965 (1411891)	1411891..1412826	-	936	WP_001157406.1	tRNA 2-thiocytidine(32) synthetase TtcA	-
LHV64_RS06970 (1412878)	1412878..1414113	-	1236	WP_000040852.1	site-specific integrase	-
LHV64_RS06975 (1414115)	1414115..1414330	-	216	WP_000079604.1	excisionase XisR	-
- (1414434)	1414434..1414612	+	179	NuclAT_0	-	Antitoxin
- (1414434)	1414434..1414612	+	179	NuclAT_0	-	Antitoxin
- (1414434)	1414434..1414612	+	179	NuclAT_0	-	Antitoxin
- (1414434)	1414434..1414612	+	179	NuclAT_0	-	Antitoxin
LHV64_RS06980 (1414409)	1414409..1414618	-	210	WP_000276809.1	double-strand break reduction protein RcbA	-
LHV64_RS06985 (1414611)	1414611..1414805	-	195	WP_001317028.1	type I toxin-antitoxin system endodeoxyribonuclease toxin RalR	Toxin
LHV64_RS06990 (1414862)	1414862..1415671	-	810	WP_000166319.1	recombination protein RecT	-
LHV64_RS06995 (1415664)	1415664..1418264	-	2601	WP_000105143.1	exodeoxyribonuclease VIII	-
LHV64_RS07000 (1418366)	1418366..1418641	-	276	WP_000632297.1	protein RacC	-
LHV64_RS07005 (1418716)	1418716..1418886	-	171	WP_001352098.1	YdaE family protein	-
LHV64_RS07010 (1418886)	1418886..1419107	-	222	WP_000560225.1	killing protein KilR	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	Similar MGE(s) Similar MGE(s) is defined as other TA-related MGE(s) with Mash distance < 0.01 to this MGE. Detail Genome accession Organism MGE type Related TA Coordinates Mash distance View CP009789 Escherichia coli K-12 strain ER3413 Prophage TA020020 1385172..1414104 0 View HG738867 Escherichia coli str. K-12 substr. MC4100 Prophage TA020061 1300529..1322203 0.008 View NC_000913 Escherichia coli str. K-12 substr. MG1655 Prophage TA006362 1412000..1433674 0.008 View NC_007779 Escherichia coli str. K-12 substr. W3110 Prophage TA021432 1413714..1435388 0.008 View NC_010473 Escherichia coli str. K-12 substr. DH10B Prophage TA022680 1499420..1521094 0.008 View NC_012759 Escherichia coli BW2952 Prophage TA023848 1302083..1323757 0.008 View NC_017638 Escherichia coli DH1 Prophage TA027398 1394182..1415856 0.008 View NZ_CP009273 Escherichia coli BW25113 Prophage TA048677 1406257..1427931 0.008 View NZ_CP025520 Escherichia coli strain DH5alpha Prophage TA090473 269442..291116 0.008 View NZ_HG738867 Escherichia coli str. K-12 substr. MC4100 strain K-12 Prophage TA285012 1300529..1322203 0.008	inside	Prophage	-	-	1405619..1434552	28933

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Endodeoxyribonuclease toxin RalR (6-54)

Sequences

Toxin

Protein

• Length: 65 a.a.
• Molecular weight: 7018.89 Da
• Isoelectric Point: 8.9538

>T219993 WP_001317028.1 NZ_CP084897:c1414805-1414611 [Escherichia coli]
MRYDNVKPCPFCGCPSVTVKAISGYYRAKCNGCESRTGYGGSEKEALERWNKRTTGNNNGGVHV

Nucleotide

• Length: 195 bp

>T219993 NZ_CP116913:2297359-2298681 [Escherichia coli]
ATGCCTAAACTTGTCACTTGGATGAACAACCAGCGGGTAGGCGAGTTAACGAAGTTAGCCAACGGCGCGCACACCTTTAA
GTATGCACCGGAGTGGTTAGCAAGCCGTTATGCCAGACCGTTGTCACTTTCGCTGCCATTGCAGAGGGGGAATATCACCT
CTGATGCCGTATTTAACTTCTTCGATAACCTGTTACCCGATAGCCCGATTGTACGTGACCGGATCGTTAAACGTTATCAT
GCCAAATCCAGACAACCGTTTGATTTATTGTCAGAAATAGGGCGAGACAGCGTTGGTGCCGTGACGTTAATACCCGAAGA
CGAAACCGTAACGCATCCGATAATGGCATGGGAAAAGCTTACTGAAGCCAGACTTGAAGAAGTATTAACGGCTTATAAAG
CAGATATCCCGCTAGGCATGATTAGAGAAGAAAATGACTTTCGCATCTCGGTTGCTGGCGCACAGGAGAAGACAGCACTG
CTCAGAATAGGCAATGACTGGTGCATTCCGAAAGGAATAACGCCGACGACGCACATCATTAAATTACCGATTGGCGAAAT
CAGGCAGCCCAATGCGACGCTCGATCTCAGCCAAAGCGTTGATAATGAGTATTACTGTCTGCTGCTGGCGAAAGAACTTG
GGTTGAATGTTCCGGACGCAGAAATCATTAAAGCGGGAAATGTGCGCGCGTTAGCGGTCGAACGTTTTGACAGGCGTTGG
AATGCTGAGCGAACGGTTTTACTTCGCTTGCCACAGGAGGATATGTGTCAGACATTCGGTTTACCTTCATCGGTGAAATA
TGAATCAGATGGAGGCCCAGGCATCGCGCGGATCATGGCTTTTTTGATGGGGTCCAGCGAGGCGCTGAAAGATCGCTATG
ATTTTATGAAATTCCAGGTCTTCCAGTGGTTGATTGGCGCAACGGACGGTCATGCAAAAAACTTCTCCGTATTTATTCAG
GCTGGCGGCAGTTATCGACTCACGCCATTTTACGACATCATTTCAGCATTTCCGGTCCTTGGCGGTACGGGAATACACAT
CAGCGATCTCAAACTGGCAATGGGGCTTAACGCATCCAAAGGCAAAAAAACGGCAATCGATAAAATTTATCCGCGACATT
TTTTGGCGACAGCAAAGGTGCTGAGATTCCCGGAAGTGCAGATGCATGAAATCCTGAGTGACTTTGCCAGAATGATTCCA
GCAGCACTGGATAACGTGAAGACTTCATTACCGACAGATTTTCCGGAGAACGTGGTGACGGCAGTTGAAAGCAATGTGTT
GAGGTTGCATGGACGGTTAAGCCGAGAATACGGTAGTAAGTGA

Antitoxin

Nucleotide

• Length: 179 bp

>AT219993 NZ_CP084897:1414434-1414612 [Escherichia coli]
GAGGACTGAAGTTTCTCGCAATTAAAATTTATCAGTTTTACTTTCTGCTCTCTGGAAACGCCTGCTTCTTTTTTACCTGA
GAGCATTTTTTCGCATTCTGATTTCGTTAGTTTAGATTTTGAATATCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTT
CAATAGTGGCGGTAATTTT

Structures

Toxin

• AlphaFold DB: A0A7L6LHV7

Antitoxin