Detailed information of TA system
Overview
TA module
| Type | I | Classification (family/domain) | ralRA/- |
| Location | 2163767..2164138 | Replicon | chromosome |
| Accession | NC_002655 | ||
| Organism | Escherichia coli O157:H7 str. EDL933 | ||
| T1TAdb ID | TA04747 | ||
Toxin (Protein)
| Gene name | ralR | Uniprot ID | A0A0D6ZRD3 |
| Locus tag | Z_RS11255 | Protein ID | WP_001443846.1 |
| Coordinates | 2163767..2163916 (+) | Length | 50 a.a. |
Antitoxin (RNA)
| Gene name | ralA | ||
| Locus tag | - | ||
| Coordinates | 2163960..2164138 (-) |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| Z_RS11230 (Z2406) | 2159466..2159687 | + | 222 | WP_000560223.1 | killing protein KilR | - |
| Z_RS11235 | 2159687..2159857 | + | 171 | WP_001352098.1 | conserved protein, Rac prophage | - |
| Z_RS11240 (Z2408) | 2159932..2160207 | + | 276 | WP_001344816.1 | hypothetical protein | - |
| Z_RS11245 (Z2409) | 2160309..2162909 | + | 2601 | WP_000105140.1 | exodeoxyribonuclease VIII | - |
| Z_RS11250 (Z2410) | 2162902..2163711 | + | 810 | WP_000166313.1 | recombination protein RecT | - |
| Z_RS11255 (Z2412) | 2163767..2163916 | + | 150 | WP_001443846.1 | type I toxin-antitoxin system endodeoxyribonuclease toxin RalR | Toxin |
| Z_RS11260 (Z2413) | 2163954..2164142 | + | 189 | WP_001302840.1 | DUF1187 family protein | - |
| - | 2163960..2164138 | - | 179 | - | - | Antitoxin |
| Z_RS11265 (Z2414) | 2164242..2164457 | + | 216 | WP_000079604.1 | excisionase XisR | - |
| Z_RS30095 (Z2415) | 2164459..2165694 | + | 1236 | WP_000040839.1 | site-specific integrase | - |
| Z_RS11275 (Z2416) | 2165746..2166681 | + | 936 | WP_001157407.1 | tRNA 2-thiocytidine(32) synthetase TtcA | - |
| Z_RS11280 (Z2417) | 2166810..2168183 | - | 1374 | WP_000123746.1 | ATP-dependent RNA helicase DbpA | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| - | inside | Prophage | - | - | 2118467..2165694 | 47227 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 50 a.a. Molecular weight: 5383.12 Da Isoelectric Point: 8.3398
>T10218 WP_001443846.1 NC_002655:2163767-2163916 [Escherichia coli O157:H7 str. EDL933]
MRYDNVKPCPFCGCPSVTVKAISGYYRAKCNGCESRTGYGGSEKEALER
MRYDNVKPCPFCGCPSVTVKAISGYYRAKCNGCESRTGYGGSEKEALER
Download Length: 150 bp
>T10218 NC_002655:2163767-2163916 [Escherichia coli O157:H7 str. EDL933]
ATGAGATATGACAATGTTAAACCATGTCCATTTTGTGGTTGTCCATCAGTAACGGTGAAAGCCATTTCAGGATATTACCG
AGCGAAGTGTAACGGATGCGAATCCCGAACCGGTTATGGTGGAAGTGAAAAAGAAGCACTCGAACGATGA
ATGAGATATGACAATGTTAAACCATGTCCATTTTGTGGTTGTCCATCAGTAACGGTGAAAGCCATTTCAGGATATTACCG
AGCGAAGTGTAACGGATGCGAATCCCGAACCGGTTATGGTGGAAGTGAAAAAGAAGCACTCGAACGATGA
Antitoxin
Download Length: 179 bp
>AT10218 NC_002655:c2164138-2163960 [Escherichia coli O157:H7 str. EDL933]
GAGGACTGAAGTTTCTCGCAATTAAAATTTATCAGTTTTACTTTCTGCTCTCTGGAAACGCCTGCTTCTTTTTTACCTGA
GAGCATTTTTTCGCATTCTGATTTCGTTAGTTTAGATTTTGAATATCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTT
CAATAGTGGCGGTAATTTT
GAGGACTGAAGTTTCTCGCAATTAAAATTTATCAGTTTTACTTTCTGCTCTCTGGAAACGCCTGCTTCTTTTTTACCTGA
GAGCATTTTTTCGCATTCTGATTTCGTTAGTTTAGATTTTGAATATCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTT
CAATAGTGGCGGTAATTTT
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|---|---|---|---|
| T6362 | Escherichia coli str. K-12 substr. MG1655 |
100 |
100 |
1 |
Multiple sequence alignment
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
References
(1) Yunxue Guo et al. (2014) RalR (a DNase) and RalA (a small RNA) form a type I toxin-antitoxin system in Escherichia coli. Nucleic Acids Research 42(10):6448-62. [PubMed:24748661]