Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type I Classification (family/domain) ralRA/-
Location 1295337..1295708 Replicon chromosome
Accession NZ_CP092037
Organism Escherichia coli strain MG1655-377GD

Toxin (Protein)


Gene name ralR Uniprot ID F4VC37
Locus tag MBA16_RS06385 Protein ID WP_001317028.1
Coordinates 1295514..1295708 (-) Length 65 a.a.

Antitoxin (RNA)


Gene name ralA
Locus tag -
Coordinates 1295337..1295515 (+)

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MBA16_RS06340 (1291638) 1291638..1291868 - 231 Protein_1229 diguanylate cyclase -
MBA16_RS06345 (1292080) 1292080..1292127 + 48 WP_211180494.1 protein YnaN -
MBA16_RS21000 (1292141) 1292141..1292464 + 324 Protein_1231 DEAD/DEAH box helicase -
MBA16_RS06360 (1292471) 1292471..1292665 + 195 Protein_1232 DbpA RNA binding domain-containing protein -
MBA16_RS06365 (1292794) 1292794..1293729 - 936 WP_001157406.1 tRNA 2-thiocytidine(32) synthetase TtcA -
MBA16_RS06370 (1293781) 1293781..1295016 - 1236 WP_000040852.1 site-specific integrase -
MBA16_RS06375 (1295018) 1295018..1295233 - 216 WP_000079604.1 excisionase XisR -
- (1295337) 1295337..1295515 + 179 NuclAT_0 - Antitoxin
- (1295337) 1295337..1295515 + 179 NuclAT_0 - Antitoxin
- (1295337) 1295337..1295515 + 179 NuclAT_0 - Antitoxin
- (1295337) 1295337..1295515 + 179 NuclAT_0 - Antitoxin
MBA16_RS06380 (1295312) 1295312..1295521 - 210 WP_000276809.1 double-strand break reduction protein RcbA -
MBA16_RS06385 (1295514) 1295514..1295708 - 195 WP_001317028.1 type I toxin-antitoxin system endodeoxyribonuclease toxin RalR Toxin
MBA16_RS06390 (1295765) 1295765..1296574 - 810 WP_000166319.1 recombination protein RecT -
MBA16_RS06395 (1296567) 1296567..1299167 - 2601 WP_000105143.1 exodeoxyribonuclease VIII -
MBA16_RS06400 (1299269) 1299269..1299544 - 276 WP_000632297.1 protein RacC -
MBA16_RS06405 (1299619) 1299619..1299789 - 171 WP_001352098.1 YdaE family protein -
MBA16_RS06410 (1299789) 1299789..1300010 - 222 WP_000560225.1 killing protein KilR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Prophage - - 1293781..1315455 21674


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Endodeoxyribonuclease toxin RalR

(6-54)


Sequences

Toxin        


Download         Length: 65 a.a.        Molecular weight: 7018.89 Da        Isoelectric Point: 8.9538

>T235505 WP_001317028.1 NZ_CP092037:c1295708-1295514 [Escherichia coli]
MRYDNVKPCPFCGCPSVTVKAISGYYRAKCNGCESRTGYGGSEKEALERWNKRTTGNNNGGVHV

Download         Length: 195 bp

>T235505 NZ_LR134296:665971-666285 [Escherichia coli]
ATGCACCTGATAACTCAAAAAGCATTGAAAGATGCTGCGGAAAAATACCCGCAACATAAAACGGAGTTGGTGGCTCTGGG
GAACACGATTGCTAAGGGATATTTCAAAAAACCTGAGTCATTAAAAGCAGTATTCCCATCTCTGGATAACTTCAAATATC
TGGATAAGCATTATGTTTTCAATGTTGGGGGCAATGAATTACGTGTTGTAGCAATGGTCTTTTTTGAATCGCAAAAGTGC
TACATACGTGAAGTTATGACGCATAAAGAATACGATTTCTTTACCGCTGTTCATCGTACTAAGGGGAAAAAATGA

Antitoxin


Download         Length: 179 bp

>AT235505 NZ_CP092037:1295337-1295515 [Escherichia coli]
GAGGACTGAAGTTTCTCGCAATTAAAATTTATCAGTTTTACTTTCTGCTCTCTGGAAACGCCTGCTTCTTTTTTACCTGA
GAGCATTTTTTCGCATTCTGATTTCGTTAGTTTAGATTTTGAATATCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTT
CAATAGTGGCGGTAATTTT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7L6LHV7

Antitoxin

Download structure file

References