Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type I Classification (family/domain) ralRA/-
Location 2655658..2656029 Replicon chromosome
Accession NZ_CP068281
Organism Escherichia coli strain CY706

Toxin (Protein)


Gene name ralR Uniprot ID F4VC37
Locus tag JJW04_RS12965 Protein ID WP_001317028.1
Coordinates 2655835..2656029 (-) Length 65 a.a.

Antitoxin (RNA)


Gene name ralA
Locus tag -
Coordinates 2655658..2655836 (+)

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JJW04_RS12940 (2651613) 2651613..2652986 + 1374 WP_000123737.1 ATP-dependent RNA helicase DbpA -
JJW04_RS12945 (2653115) 2653115..2654050 - 936 WP_001157406.1 tRNA 2-thiocytidine(32) synthetase TtcA -
JJW04_RS12950 (2654102) 2654102..2655337 - 1236 WP_000040852.1 site-specific integrase -
JJW04_RS12955 (2655339) 2655339..2655554 - 216 WP_000079604.1 excisionase XisR -
- (2655658) 2655658..2655836 + 179 NuclAT_0 - Antitoxin
- (2655658) 2655658..2655836 + 179 NuclAT_0 - Antitoxin
- (2655658) 2655658..2655836 + 179 NuclAT_0 - Antitoxin
- (2655658) 2655658..2655836 + 179 NuclAT_0 - Antitoxin
JJW04_RS12960 (2655633) 2655633..2655842 - 210 WP_000276809.1 double-strand break reduction protein RcbA -
JJW04_RS12965 (2655835) 2655835..2656029 - 195 WP_001317028.1 type I toxin-antitoxin system endodeoxyribonuclease toxin RalR Toxin
JJW04_RS12970 (2656086) 2656086..2656895 - 810 WP_000166319.1 recombination protein RecT -
JJW04_RS12975 (2656888) 2656888..2659488 - 2601 WP_000105143.1 exodeoxyribonuclease VIII -
JJW04_RS12980 (2659590) 2659590..2659865 - 276 WP_000632297.1 protein RacC -
JJW04_RS12985 (2659940) 2659940..2660110 - 171 WP_001352098.1 YdaE family protein -
JJW04_RS12990 (2660110) 2660110..2660331 - 222 WP_000560225.1 killing protein KilR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Prophage - - 2654102..2675776 21674


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Endodeoxyribonuclease toxin RalR

(6-54)


Sequences

Toxin        


Download         Length: 65 a.a.        Molecular weight: 7018.89 Da        Isoelectric Point: 8.9538

>T187052 WP_001317028.1 NZ_CP068281:c2656029-2655835 [Escherichia coli]
MRYDNVKPCPFCGCPSVTVKAISGYYRAKCNGCESRTGYGGSEKEALERWNKRTTGNNNGGVHV

Download         Length: 195 bp

>T187052 NZ_CP089777:c646397-645984 [Mycobacterium tuberculosis]
GTGAGAGCATCGCCCACTTCGCCGCCGGAGCAGGTGGTCGTCGACGCGAGTGCCATGGTGGATCTACTGGCTCGCACTAG
CGATCGGTGCTCTGCGGTGCGCGCGCGGCTGGCTCGGACCGCGATGCACGCGCCGGCGCACTTCGATGCAGAGGTGTTGT
CGGCGCTGGGGCGCATGCAGCGCGCCGGCGCACTCACCGTTGCCTATGTCGATGCGGCACTGGAGGAGTTGCGACAGGTG
CCGGTGACTCGACACGGTCTTTCGTCGCTGCTTGCTGGAGCGTGGTCGCGCCGCGACACCCTCCGCCTGACCGATGCCCT
CTACGTCGAGCTGGCCGAAACGGCAGGTCTGGTGTTGTTGACCACCGACGAAAGATTGGCACGCGCCTGGCCCTCGGCTC
ACGCCATCGGCTGA

Antitoxin


Download         Length: 179 bp

>AT187052 NZ_CP068281:2655658-2655836 [Escherichia coli]
GAGGACTGAAGTTTCTCGCAATTAAAATTTATCAGTTTTACTTTCTGCTCTCTGGAAACGCCTGCTTCTTTTTTACCTGA
GAGCATTTTTTCGCATTCTGATTTCGTTAGTTTAGATTTTGAATATCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTT
CAATAGTGGCGGTAATTTT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7L6LHV7

Antitoxin

Download structure file

References