Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type I Classification (family/domain) ralRA/-
Location 1331034..1331405 Replicon chromosome
Accession NZ_CP077969
Organism Escherichia coli strain JM83

Toxin (Protein)


Gene name ralR Uniprot ID F4VC37
Locus tag KTJ86_RS06525 Protein ID WP_001317028.1
Coordinates 1331211..1331405 (-) Length 65 a.a.

Antitoxin (RNA)


Gene name ralA
Locus tag -
Coordinates 1331034..1331212 (+)

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KTJ86_RS06500 (1326989) 1326989..1328362 + 1374 WP_000123737.1 ATP-dependent RNA helicase DbpA -
KTJ86_RS06505 (1328491) 1328491..1329426 - 936 WP_001157406.1 tRNA 2-thiocytidine(32) synthetase TtcA -
KTJ86_RS06510 (1329478) 1329478..1330713 - 1236 WP_000040852.1 site-specific integrase -
KTJ86_RS06515 (1330715) 1330715..1330930 - 216 WP_000079604.1 excisionase XisR -
- (1331034) 1331034..1331212 + 179 NuclAT_0 - Antitoxin
- (1331034) 1331034..1331212 + 179 NuclAT_0 - Antitoxin
- (1331034) 1331034..1331212 + 179 NuclAT_0 - Antitoxin
- (1331034) 1331034..1331212 + 179 NuclAT_0 - Antitoxin
KTJ86_RS06520 (1331009) 1331009..1331218 - 210 WP_000276809.1 double-strand break reduction protein RcbA -
KTJ86_RS06525 (1331211) 1331211..1331405 - 195 WP_001317028.1 type I toxin-antitoxin system endodeoxyribonuclease toxin RalR Toxin
KTJ86_RS06530 (1331462) 1331462..1332271 - 810 WP_000166319.1 recombination protein RecT -
KTJ86_RS06535 (1332264) 1332264..1334864 - 2601 WP_000105143.1 exodeoxyribonuclease VIII -
KTJ86_RS06540 (1334966) 1334966..1335241 - 276 WP_000632297.1 protein RacC -
KTJ86_RS06545 (1335316) 1335316..1335486 - 171 WP_001352098.1 YdaE family protein -
KTJ86_RS06550 (1335486) 1335486..1335707 - 222 WP_000560225.1 killing protein KilR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Prophage - - 1329478..1351152 21674


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Endodeoxyribonuclease toxin RalR

(6-54)


Sequences

Toxin        


Download         Length: 65 a.a.        Molecular weight: 7018.89 Da        Isoelectric Point: 8.9538

>T208751 WP_001317028.1 NZ_CP077969:c1331405-1331211 [Escherichia coli]
MRYDNVKPCPFCGCPSVTVKAISGYYRAKCNGCESRTGYGGSEKEALERWNKRTTGNNNGGVHV

Download         Length: 195 bp

>T208751 NZ_CP103718:c4926046-4925735 [Escherichia coli]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGACCGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTGTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin


Download         Length: 179 bp

>AT208751 NZ_CP077969:1331034-1331212 [Escherichia coli]
GAGGACTGAAGTTTCTCGCAATTAAAATTTATCAGTTTTACTTTCTGCTCTCTGGAAACGCCTGCTTCTTTTTTACCTGA
GAGCATTTTTTCGCATTCTGATTTCGTTAGTTTAGATTTTGAATATCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTT
CAATAGTGGCGGTAATTTT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7L6LHV7

Antitoxin

Download structure file

References