Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type I Classification (family/domain) ralRA/-
Location 141815..142186 Replicon chromosome
Accession NZ_CP043185
Organism Escherichia coli O16:H48 strain PG20180171

Toxin (Protein)


Gene name ralR Uniprot ID F4VC37
Locus tag FYA18_RS00720 Protein ID WP_001317028.1
Coordinates 141992..142186 (-) Length 65 a.a.

Antitoxin (RNA)


Gene name ralA
Locus tag -
Coordinates 141815..141993 (+)

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FYA18_RS00695 137770..139143 + 1374 WP_000123737.1 ATP-dependent RNA helicase DbpA -
FYA18_RS00700 139272..140207 - 936 WP_001157406.1 tRNA 2-thiocytidine(32) synthetase TtcA -
FYA18_RS00705 140259..141494 - 1236 WP_000040852.1 site-specific integrase -
FYA18_RS00710 141496..141711 - 216 WP_000079604.1 excisionase XisR -
FYA18_RS00715 141790..141999 - 210 WP_000276809.1 double-strand break reduction protein RcbA -
- 141815..141993 + 179 NuclAT_0 - Antitoxin
- 141815..141993 + 179 NuclAT_0 - Antitoxin
- 141815..141993 + 179 NuclAT_0 - Antitoxin
- 141815..141993 + 179 NuclAT_0 - Antitoxin
FYA18_RS00720 141992..142186 - 195 WP_001317028.1 type I toxin-antitoxin system endodeoxyribonuclease toxin RalR Toxin
FYA18_RS00725 142243..143052 - 810 WP_000166319.1 recombination protein RecT -
FYA18_RS00730 143045..145645 - 2601 WP_000105143.1 exodeoxyribonuclease VIII -
FYA18_RS00735 145747..146022 - 276 WP_000632297.1 protein RacC -
FYA18_RS00740 146097..146267 - 171 WP_001352098.1 conserved protein, Rac prophage -
FYA18_RS00745 146267..146488 - 222 WP_000560225.1 killing protein KilR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Prophage - - 140259..161933 21674


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Endodeoxyribonuclease toxin RalR

(6-54)


Sequences

Toxin        


Download         Length: 65 a.a.        Molecular weight: 7018.89 Da        Isoelectric Point: 8.9538

>T137422 WP_001317028.1 NZ_CP043185:c142186-141992 [Escherichia coli O16:H48]
MRYDNVKPCPFCGCPSVTVKAISGYYRAKCNGCESRTGYGGSEKEALERWNKRTTGNNNGGVHV

Download         Length: 195 bp

>T137422 NZ_CP056729:c5341-5036 [Enterobacter hormaechei]
ATGCAGTTTAAGGTTTATGCCTATAAAAGAGAGAGCCGCTATCGTCTGTTCGTGGATGTGCAGAGTGACATCATCGATAC
CCCCGGGCGGCGGATGGTGATCCCGCTGGCCAGCGCCCGGTTGCTGTCGGATAAGGTCTCCCGTGAGCTCTATCCGGTGG
TGCACATCGGGGACGAGAGCTGGCGCCTGATGACCACGGACATGGCCAGCGTCCCGGCCTCCGTCACCGGGGAGGAAGTG
GCCGATCTCCGCCACCGGGAAAATGACATCAAAAACGCAATTAACCTGATGTTCTGGGGGATCTAA

Antitoxin


Download         Length: 179 bp

>AT137422 NZ_CP043185:141815-141993 [Escherichia coli O16:H48]
GAGGACTGAAGTTTCTCGCAATTAAAATTTATCAGTTTTACTTTCTGCTCTCTGGAAACGCCTGCTTCTTTTTTACCTGA
GAGCATTTTTTCGCATTCTGATTTCGTTAGTTTAGATTTTGAATATCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTT
CAATAGTGGCGGTAATTTT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7L6LHV7

Antitoxin

Download structure file

References