Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type I Classification (family/domain) ralRA/-
Location 2119114..2119485 Replicon chromosome
Accession NZ_CP044410
Organism Escherichia coli strain ecMN1F

Toxin (Protein)


Gene name ralR Uniprot ID F4VC37
Locus tag FNW43_RS10265 Protein ID WP_001317028.1
Coordinates 2119291..2119485 (-) Length 65 a.a.

Antitoxin (RNA)


Gene name ralA
Locus tag -
Coordinates 2119114..2119292 (+)

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FNW43_RS10240 2115069..2116442 + 1374 WP_000123737.1 ATP-dependent RNA helicase DbpA -
FNW43_RS10245 2116571..2117506 - 936 WP_001157406.1 tRNA 2-thiocytidine(32) synthetase TtcA -
FNW43_RS10250 2117558..2118793 - 1236 WP_000040852.1 site-specific integrase -
FNW43_RS10255 2118795..2119010 - 216 WP_000079604.1 excisionase XisR -
FNW43_RS10260 2119089..2119298 - 210 WP_000276809.1 double-strand break reduction protein RcbA -
- 2119114..2119292 + 179 NuclAT_0 - Antitoxin
- 2119114..2119292 + 179 NuclAT_0 - Antitoxin
- 2119114..2119292 + 179 NuclAT_0 - Antitoxin
- 2119114..2119292 + 179 NuclAT_0 - Antitoxin
FNW43_RS10265 2119291..2119485 - 195 WP_001317028.1 type I toxin-antitoxin system endodeoxyribonuclease toxin RalR Toxin
FNW43_RS10270 2119542..2120351 - 810 WP_000166319.1 recombination protein RecT -
FNW43_RS10275 2120344..2122944 - 2601 WP_000105143.1 exodeoxyribonuclease VIII -
FNW43_RS10280 2123046..2123321 - 276 WP_000632297.1 protein RacC -
FNW43_RS10285 2123396..2123566 - 171 WP_001352098.1 conserved protein, Rac prophage -
FNW43_RS10290 2123566..2123787 - 222 WP_000560225.1 killing protein KilR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Prophage - - 2110299..2139231 28932


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Endodeoxyribonuclease toxin RalR

(6-54)


Sequences

Toxin        


Download         Length: 65 a.a.        Molecular weight: 7018.89 Da        Isoelectric Point: 8.9538

>T140887 WP_001317028.1 NZ_CP044410:c2119485-2119291 [Escherichia coli]
MRYDNVKPCPFCGCPSVTVKAISGYYRAKCNGCESRTGYGGSEKEALERWNKRTTGNNNGGVHV

Download         Length: 195 bp

>T140887 NZ_CP059494:1670268-1671014 [Bacillus haynesii]
ATGCTGCTATTTTATCAATTCCTTGTTTGGCTCATTGTTTTGGCGCTGTCTCTGTACGTGGCTGCCGTGTGGTGTTTTGA
AAAAAAGCTCACCGAAAAAACGGTCGCGATCCGAAAAACGTGGTACCTGCTTTATGTTATCGGCGCCGTGATTTATTGGA
CGCACGATCCGCAGTCGATTTTCACGAACCCGCTTCATTACCTGATCGTTGCCGTTTTTTTCACGCTGACAGACGCTTTT
ATTTTCTTAAACGCCTACTTTAAAAAGCTCGGCAGCTCCGAACTCGCGACAGACACAAGAATGCTTCTCGAAGAAAACAA
CGACCTCCTCCACACGTATCAAAACAGGCTGAAAACGTTTCAATACCTATTGAAAAACGAACCGATCCACATCTATTATG
GAAATATAGAAGCGTATGCAGAAGGCATCGAAAAGCTCATCAAACGGTTTGCCGAAAAAATGAATATATCCGCTGCACTT
TGCGAATATAATTCAGAAGAAAGCAAGGATCATTTGCTTGAGCATATGGAAAACCGGTTCGATGTTCAAGAAAAGCTTGA
GCGCAAAGACGTTTATTATGAAGAAAACGGAAAAATGGTCCTCATTCCTTTTTCCATCCACGATTTTGACTATGTCATGA
AGTTAACCTCAGAAGACCTTGTCACAGAATTTGATTATCTCCTGTTTACTTCTTTAACAAGCATCTATGATCTTCTGCTA
CCAAACGAAGAGGAAGGTGACGACTGA

Antitoxin


Download         Length: 179 bp

>AT140887 NZ_CP044410:2119114-2119292 [Escherichia coli]
GAGGACTGAAGTTTCTCGCAATTAAAATTTATCAGTTTTACTTTCTGCTCTCTGGAAACGCCTGCTTCTTTTTTACCTGA
GAGCATTTTTTCGCATTCTGATTTCGTTAGTTTAGATTTTGAATATCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTT
CAATAGTGGCGGTAATTTT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7L6LHV7

Antitoxin

Download structure file

References