Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type I Classification (family/domain) ralRA/-
Location 4252480..4252851 Replicon chromosome
Accession NZ_CP014225
Organism Escherichia coli str. K-12 substr. MG1655 strain K-12

Toxin (Protein)


Gene name ralR Uniprot ID F4VC37
Locus tag AW869_RS21065 Protein ID WP_001317028.1
Coordinates 4252657..4252851 (-) Length 65 a.a.

Antitoxin (RNA)


Gene name ralA
Locus tag -
Coordinates 4252480..4252658 (+)

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AW869_RS21040 4248435..4249808 + 1374 WP_000123737.1 ATP-dependent RNA helicase DbpA -
AW869_RS21045 4249937..4250872 - 936 WP_001157406.1 tRNA 2-thiocytidine(32) synthetase TtcA -
AW869_RS24720 4250924..4252159 - 1236 WP_000040852.1 site-specific integrase -
AW869_RS21055 4252161..4252376 - 216 WP_000079604.1 excisionase XisR -
AW869_RS21060 4252455..4252664 - 210 WP_000276809.1 double-strand break reduction protein RcbA -
- 4252480..4252658 + 179 NuclAT_0 - Antitoxin
- 4252480..4252658 + 179 NuclAT_0 - Antitoxin
- 4252480..4252658 + 179 NuclAT_0 - Antitoxin
- 4252480..4252658 + 179 NuclAT_0 - Antitoxin
AW869_RS21065 4252657..4252851 - 195 WP_001317028.1 type I toxin-antitoxin system endodeoxyribonuclease toxin RalR Toxin
AW869_RS21070 4252908..4253717 - 810 WP_000166319.1 recombination protein RecT -
AW869_RS21075 4253710..4256310 - 2601 WP_000105143.1 exodeoxyribonuclease VIII -
AW869_RS21080 4256412..4256687 - 276 WP_000632297.1 protein RacC -
AW869_RS21085 4256762..4256932 - 171 WP_001352098.1 conserved protein, Rac prophage -
AW869_RS21090 4256932..4257153 - 222 WP_000560225.1 killing protein KilR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Prophage - - 4250924..4272598 21674


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Endodeoxyribonuclease toxin RalR

(6-54)


Sequences

Toxin        


Download         Length: 65 a.a.        Molecular weight: 7018.89 Da        Isoelectric Point: 8.9538

>T60408 WP_001317028.1 NZ_CP014225:c4252851-4252657 [Escherichia coli str. K-12 substr. MG1655]
MRYDNVKPCPFCGCPSVTVKAISGYYRAKCNGCESRTGYGGSEKEALERWNKRTTGNNNGGVHV

Download         Length: 195 bp

>T60408 NZ_CP014225:c4252851-4252657 [Escherichia coli str. K-12 substr. MG1655]
ATGAGATATGACAATGTTAAACCATGTCCATTTTGTGGTTGTCCATCAGTAACGGTGAAAGCCATTTCAGGATATTACCG
AGCGAAGTGTAACGGATGCGAATCCCGAACCGGTTATGGTGGAAGTGAAAAAGAAGCACTCGAAAGATGGAATAAACGAA
CCACTGGAAATAATAATGGAGGTGTTCATGTATAA

Antitoxin


Download         Length: 179 bp

>AT60408 NZ_CP014225:4252480-4252658 [Escherichia coli str. K-12 substr. MG1655]
GAGGACTGAAGTTTCTCGCAATTAAAATTTATCAGTTTTACTTTCTGCTCTCTGGAAACGCCTGCTTCTTTTTTACCTGA
GAGCATTTTTTCGCATTCTGATTTCGTTAGTTTAGATTTTGAATATCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTT
CAATAGTGGCGGTAATTTT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7L6LHV7

Antitoxin

Download structure file

References