Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type I Classification (family/domain) ralRA/-
Location 1380127..1380498 Replicon chromosome
Accession NZ_CP034658
Organism Escherichia coli strain ATCC 98082

Toxin (Protein)


Gene name ralR Uniprot ID F4VC37
Locus tag EKH78_RS06710 Protein ID WP_001317028.1
Coordinates 1380304..1380498 (-) Length 65 a.a.

Antitoxin (RNA)


Gene name ralA
Locus tag -
Coordinates 1380127..1380305 (+)

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EKH78_RS24895 (1375879) 1375879..1376052 + 174 WP_001296046.1 protein YnaL -
EKH78_RS06685 (1376082) 1376082..1377455 + 1374 WP_000123737.1 ATP-dependent RNA helicase DbpA -
EKH78_RS06690 (1377584) 1377584..1378519 - 936 WP_001157406.1 tRNA 2-thiocytidine(32) synthetase TtcA -
EKH78_RS06695 (1378571) 1378571..1379806 - 1236 WP_000040852.1 site-specific integrase -
EKH78_RS06700 (1379808) 1379808..1380023 - 216 WP_000079604.1 excisionase XisR -
- (1380127) 1380127..1380305 + 179 NuclAT_0 - Antitoxin
- (1380127) 1380127..1380305 + 179 NuclAT_0 - Antitoxin
- (1380127) 1380127..1380305 + 179 NuclAT_0 - Antitoxin
- (1380127) 1380127..1380305 + 179 NuclAT_0 - Antitoxin
EKH78_RS06705 (1380102) 1380102..1380311 - 210 WP_000276809.1 double-strand break reduction protein RcbA -
EKH78_RS06710 (1380304) 1380304..1380498 - 195 WP_001317028.1 type I toxin-antitoxin system endodeoxyribonuclease toxin RalR Toxin
EKH78_RS06715 (1380555) 1380555..1381364 - 810 WP_000166319.1 recombination protein RecT -
EKH78_RS06720 (1381357) 1381357..1383957 - 2601 WP_000105143.1 exodeoxyribonuclease VIII -
EKH78_RS06725 (1384059) 1384059..1384334 - 276 WP_000632297.1 protein RacC -
EKH78_RS06730 (1384409) 1384409..1384579 - 171 WP_001352098.1 YdaE family protein -
EKH78_RS06735 (1384579) 1384579..1384800 - 222 WP_000560225.1 killing protein KilR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Prophage - - 1378571..1400245 21674


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Endodeoxyribonuclease toxin RalR

(6-54)


Sequences

Toxin        


Download         Length: 65 a.a.        Molecular weight: 7018.89 Da        Isoelectric Point: 8.9538

>T116308 WP_001317028.1 NZ_CP034658:c1380498-1380304 [Escherichia coli]
MRYDNVKPCPFCGCPSVTVKAISGYYRAKCNGCESRTGYGGSEKEALERWNKRTTGNNNGGVHV

Download         Length: 195 bp

>T116308 NZ_CP034658:c1380498-1380304 [Escherichia coli]
ATGAGATATGACAATGTTAAACCATGTCCATTTTGTGGTTGTCCATCAGTAACGGTGAAAGCCATTTCAGGATATTACCG
AGCGAAGTGTAACGGATGCGAATCCCGAACCGGTTATGGTGGAAGTGAAAAAGAAGCACTCGAAAGATGGAATAAACGAA
CCACTGGAAATAATAATGGAGGTGTTCATGTATAA

Antitoxin


Download         Length: 179 bp

>AT116308 NZ_CP034658:1380127-1380305 [Escherichia coli]
GAGGACTGAAGTTTCTCGCAATTAAAATTTATCAGTTTTACTTTCTGCTCTCTGGAAACGCCTGCTTCTTTTTTACCTGA
GAGCATTTTTTCGCATTCTGATTTCGTTAGTTTAGATTTTGAATATCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTT
CAATAGTGGCGGTAATTTT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7L6LHV7

Antitoxin

Download structure file

References