Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type I Classification (family/domain) ralRA/-
Location 1318661..1319032 Replicon chromosome
Accession NZ_CP014270
Organism Escherichia coli K-12 strain K-12 DHB4

Toxin (Protein)


Gene name ralR Uniprot ID F4VC37
Locus tag DHB4_RS06640 Protein ID WP_001317028.1
Coordinates 1318838..1319032 (-) Length 65 a.a.

Antitoxin (RNA)


Gene name ralA
Locus tag -
Coordinates 1318661..1318839 (+)

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
DHB4_RS06615 1314616..1315989 + 1374 WP_000123737.1 ATP-dependent RNA helicase DbpA -
DHB4_RS06620 1316118..1317053 - 936 WP_001157406.1 tRNA 2-thiocytidine(32) synthetase TtcA -
DHB4_RS24355 1317105..1318340 - 1236 WP_000040852.1 site-specific integrase -
DHB4_RS06630 1318342..1318557 - 216 WP_000079604.1 excisionase XisR -
DHB4_RS06635 1318636..1318845 - 210 WP_000276809.1 double-strand break reduction protein RcbA -
- 1318661..1318839 + 179 NuclAT_0 - Antitoxin
- 1318661..1318839 + 179 NuclAT_0 - Antitoxin
- 1318661..1318839 + 179 NuclAT_0 - Antitoxin
- 1318661..1318839 + 179 NuclAT_0 - Antitoxin
DHB4_RS06640 1318838..1319032 - 195 WP_001317028.1 type I toxin-antitoxin system endodeoxyribonuclease toxin RalR Toxin
DHB4_RS06645 1319089..1319898 - 810 WP_000166319.1 recombination protein RecT -
DHB4_RS06650 1319891..1322491 - 2601 WP_000105143.1 exodeoxyribonuclease VIII -
DHB4_RS06655 1322593..1322868 - 276 WP_000632297.1 protein RacC -
DHB4_RS06660 1322943..1323113 - 171 WP_001352098.1 conserved protein, Rac prophage -
DHB4_RS06665 1323113..1323334 - 222 WP_000560225.1 killing protein KilR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Prophage - - 1317105..1338779 21674


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Endodeoxyribonuclease toxin RalR

(6-54)


Sequences

Toxin        


Download         Length: 65 a.a.        Molecular weight: 7018.89 Da        Isoelectric Point: 8.9538

>T60521 WP_001317028.1 NZ_CP014270:c1319032-1318838 [Escherichia coli K-12]
MRYDNVKPCPFCGCPSVTVKAISGYYRAKCNGCESRTGYGGSEKEALERWNKRTTGNNNGGVHV

Download         Length: 195 bp

>T60521 NZ_CP014270:c1319032-1318838 [Escherichia coli K-12]
ATGAGATATGACAATGTTAAACCATGTCCATTTTGTGGTTGTCCATCAGTAACGGTGAAAGCCATTTCAGGATATTACCG
AGCGAAGTGTAACGGATGCGAATCCCGAACCGGTTATGGTGGAAGTGAAAAAGAAGCACTCGAAAGATGGAATAAACGAA
CCACTGGAAATAATAATGGAGGTGTTCATGTATAA

Antitoxin


Download         Length: 179 bp

>AT60521 NZ_CP014270:1318661-1318839 [Escherichia coli K-12]
GAGGACTGAAGTTTCTCGCAATTAAAATTTATCAGTTTTACTTTCTGCTCTCTGGAAACGCCTGCTTCTTTTTTACCTGA
GAGCATTTTTTCGCATTCTGATTTCGTTAGTTTAGATTTTGAATATCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTT
CAATAGTGGCGGTAATTTT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7L6LHV7

Antitoxin

Download structure file

References