Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type I Classification (family/domain) ralRA/-
Location 2117932..2118303 Replicon chromosome
Accession NZ_CP042845
Organism Escherichia coli strain JME65

Toxin (Protein)


Gene name ralR Uniprot ID F4VC37
Locus tag FVE21_RS10315 Protein ID WP_001317028.1
Coordinates 2118109..2118303 (-) Length 65 a.a.

Antitoxin (RNA)


Gene name ralA
Locus tag -
Coordinates 2117932..2118110 (+)

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FVE21_RS10290 2113887..2115260 + 1374 WP_000123737.1 ATP-dependent RNA helicase DbpA -
FVE21_RS10295 2115389..2116324 - 936 WP_001157406.1 tRNA 2-thiocytidine(32) synthetase TtcA -
FVE21_RS10300 2116376..2117611 - 1236 WP_000040852.1 site-specific integrase -
FVE21_RS10305 2117613..2117828 - 216 WP_000079604.1 excisionase XisR -
FVE21_RS10310 2117907..2118116 - 210 WP_000276809.1 double-strand break reduction protein RcbA -
- 2117932..2118110 + 179 NuclAT_0 - Antitoxin
- 2117932..2118110 + 179 NuclAT_0 - Antitoxin
- 2117932..2118110 + 179 NuclAT_0 - Antitoxin
- 2117932..2118110 + 179 NuclAT_0 - Antitoxin
FVE21_RS10315 2118109..2118303 - 195 WP_001317028.1 type I toxin-antitoxin system endodeoxyribonuclease toxin RalR Toxin
FVE21_RS10320 2118360..2119169 - 810 WP_000166319.1 recombination protein RecT -
FVE21_RS10325 2119162..2121762 - 2601 WP_000105143.1 exodeoxyribonuclease VIII -
FVE21_RS10330 2121864..2122139 - 276 WP_000632297.1 protein RacC -
FVE21_RS10335 2122214..2122384 - 171 WP_001352098.1 conserved protein, Rac prophage -
FVE21_RS10340 2122384..2122605 - 222 WP_000560225.1 killing protein KilR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Prophage - - 2116376..2138050 21674


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Endodeoxyribonuclease toxin RalR

(6-54)


Sequences

Toxin        


Download         Length: 65 a.a.        Molecular weight: 7018.89 Da        Isoelectric Point: 8.9538

>T136171 WP_001317028.1 NZ_CP042845:c2118303-2118109 [Escherichia coli]
MRYDNVKPCPFCGCPSVTVKAISGYYRAKCNGCESRTGYGGSEKEALERWNKRTTGNNNGGVHV

Download         Length: 195 bp

>T136171 NZ_CP042845:c2118303-2118109 [Escherichia coli]
ATGAGATATGACAATGTTAAACCATGTCCATTTTGTGGTTGTCCATCAGTAACGGTGAAAGCCATTTCAGGATATTACCG
AGCGAAGTGTAACGGATGCGAATCCCGAACCGGTTATGGTGGAAGTGAAAAAGAAGCACTCGAAAGATGGAATAAACGAA
CCACTGGAAATAATAATGGAGGTGTTCATGTATAA

Antitoxin


Download         Length: 179 bp

>AT136171 NZ_CP042845:2117932-2118110 [Escherichia coli]
GAGGACTGAAGTTTCTCGCAATTAAAATTTATCAGTTTTACTTTCTGCTCTCTGGAAACGCCTGCTTCTTTTTTACCTGA
GAGCATTTTTTCGCATTCTGATTTCGTTAGTTTAGATTTTGAATATCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTT
CAATAGTGGCGGTAATTTT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7L6LHV7

Antitoxin

Download structure file

References