Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type I Classification (family/domain) ralRA/-
Location 3542491..3542862 Replicon chromosome
Accession NZ_CP030240
Organism Escherichia coli strain ER1709

Toxin (Protein)


Gene name ralR Uniprot ID F4VC37
Locus tag DQ225_RS17195 Protein ID WP_001317028.1
Coordinates 3542668..3542862 (-) Length 65 a.a.

Antitoxin (RNA)


Gene name ralA
Locus tag -
Coordinates 3542491..3542669 (+)

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
DQ225_RS17170 3538446..3539819 + 1374 WP_000123737.1 ATP-dependent RNA helicase DbpA -
DQ225_RS17175 3539948..3540883 - 936 WP_001157406.1 tRNA 2-thiocytidine(32) synthetase TtcA -
DQ225_RS17180 3540935..3542170 - 1236 WP_000040852.1 site-specific integrase -
DQ225_RS17185 3542172..3542387 - 216 WP_000079604.1 excisionase XisR -
DQ225_RS17190 3542466..3542675 - 210 WP_000276809.1 double-strand break reduction protein RcbA -
- 3542491..3542669 + 179 NuclAT_0 - Antitoxin
- 3542491..3542669 + 179 NuclAT_0 - Antitoxin
- 3542491..3542669 + 179 NuclAT_0 - Antitoxin
- 3542491..3542669 + 179 NuclAT_0 - Antitoxin
DQ225_RS17195 3542668..3542862 - 195 WP_001317028.1 type I toxin-antitoxin system endodeoxyribonuclease toxin RalR Toxin
DQ225_RS17200 3542919..3543728 - 810 WP_000166319.1 recombination protein RecT -
DQ225_RS17205 3543721..3546321 - 2601 WP_000105143.1 exodeoxyribonuclease VIII -
DQ225_RS17210 3546423..3546698 - 276 WP_000632297.1 protein RacC -
DQ225_RS17215 3546773..3546943 - 171 WP_001352098.1 conserved protein, Rac prophage -
DQ225_RS17220 3546943..3547164 - 222 WP_000560225.1 killing protein KilR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Prophage - - 3540935..3562607 21672


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Endodeoxyribonuclease toxin RalR

(6-54)


Sequences

Toxin        


Download         Length: 65 a.a.        Molecular weight: 7018.89 Da        Isoelectric Point: 8.9538

>T107385 WP_001317028.1 NZ_CP030240:c3542862-3542668 [Escherichia coli]
MRYDNVKPCPFCGCPSVTVKAISGYYRAKCNGCESRTGYGGSEKEALERWNKRTTGNNNGGVHV

Download         Length: 195 bp

>T107385 NZ_CP030240:c3542862-3542668 [Escherichia coli]
ATGAGATATGACAATGTTAAACCATGTCCATTTTGTGGTTGTCCATCAGTAACGGTGAAAGCCATTTCAGGATATTACCG
AGCGAAGTGTAACGGATGCGAATCCCGAACCGGTTATGGTGGAAGTGAAAAAGAAGCACTCGAAAGATGGAATAAACGAA
CCACTGGAAATAATAATGGAGGTGTTCATGTATAA

Antitoxin


Download         Length: 179 bp

>AT107385 NZ_CP030240:3542491-3542669 [Escherichia coli]
GAGGACTGAAGTTTCTCGCAATTAAAATTTATCAGTTTTACTTTCTGCTCTCTGGAAACGCCTGCTTCTTTTTTACCTGA
GAGCATTTTTTCGCATTCTGATTTCGTTAGTTTAGATTTTGAATATCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTT
CAATAGTGGCGGTAATTTT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7L6LHV7

Antitoxin

Download structure file

References