Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type I Classification (family/domain) ralRA/-
Location 2271764..2272135 Replicon chromosome
Accession NZ_CP028703
Organism Escherichia coli strain ME8067

Toxin (Protein)


Gene name ralR Uniprot ID F4VC37
Locus tag DBB47_RS11835 Protein ID WP_001317028.1
Coordinates 2271941..2272135 (-) Length 65 a.a.

Antitoxin (RNA)


Gene name ralA
Locus tag -
Coordinates 2271764..2271942 (+)

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
DBB47_RS11810 2267719..2269092 + 1374 WP_000123737.1 ATP-dependent RNA helicase DbpA -
DBB47_RS11815 2269221..2270156 - 936 WP_001157406.1 tRNA 2-thiocytidine(32) synthetase TtcA -
DBB47_RS11820 2270208..2271443 - 1236 WP_000040852.1 site-specific integrase -
DBB47_RS11825 2271445..2271660 - 216 WP_000079604.1 excisionase XisR -
DBB47_RS11830 2271739..2271948 - 210 WP_000276809.1 double-strand break reduction protein RcbA -
- 2271764..2271942 + 179 NuclAT_0 - Antitoxin
- 2271764..2271942 + 179 NuclAT_0 - Antitoxin
- 2271764..2271942 + 179 NuclAT_0 - Antitoxin
- 2271764..2271942 + 179 NuclAT_0 - Antitoxin
DBB47_RS11835 2271941..2272135 - 195 WP_001317028.1 type I toxin-antitoxin system endodeoxyribonuclease toxin RalR Toxin
DBB47_RS11840 2272192..2273001 - 810 WP_000166319.1 recombination protein RecT -
DBB47_RS11845 2272994..2275594 - 2601 WP_000105143.1 exodeoxyribonuclease VIII -
DBB47_RS11850 2275696..2275971 - 276 WP_000632297.1 protein RacC -
DBB47_RS11855 2276046..2276216 - 171 WP_001352098.1 conserved protein, Rac prophage -
DBB47_RS11860 2276216..2276437 - 222 WP_000560225.1 killing protein KilR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Prophage - - 2270208..2291882 21674


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Endodeoxyribonuclease toxin RalR

(6-54)


Sequences

Toxin        


Download         Length: 65 a.a.        Molecular weight: 7018.89 Da        Isoelectric Point: 8.9538

>T102996 WP_001317028.1 NZ_CP028703:c2272135-2271941 [Escherichia coli]
MRYDNVKPCPFCGCPSVTVKAISGYYRAKCNGCESRTGYGGSEKEALERWNKRTTGNNNGGVHV

Download         Length: 195 bp

>T102996 NZ_CP028703:c2272135-2271941 [Escherichia coli]
ATGAGATATGACAATGTTAAACCATGTCCATTTTGTGGTTGTCCATCAGTAACGGTGAAAGCCATTTCAGGATATTACCG
AGCGAAGTGTAACGGATGCGAATCCCGAACCGGTTATGGTGGAAGTGAAAAAGAAGCACTCGAAAGATGGAATAAACGAA
CCACTGGAAATAATAATGGAGGTGTTCATGTATAA

Antitoxin


Download         Length: 179 bp

>AT102996 NZ_CP028703:2271764-2271942 [Escherichia coli]
GAGGACTGAAGTTTCTCGCAATTAAAATTTATCAGTTTTACTTTCTGCTCTCTGGAAACGCCTGCTTCTTTTTTACCTGA
GAGCATTTTTTCGCATTCTGATTTCGTTAGTTTAGATTTTGAATATCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTT
CAATAGTGGCGGTAATTTT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7L6LHV7

Antitoxin

Download structure file

References