Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type I Classification (family/domain) ralRA/-
Location 1517725..1518096 Replicon chromosome
Accession NZ_CP014272
Organism Escherichia coli K-12 strain K-12 C3026

Toxin (Protein)


Gene name ralR Uniprot ID F4VC37
Locus tag C3026_RS07870 Protein ID WP_001317028.1
Coordinates 1517902..1518096 (-) Length 65 a.a.

Antitoxin (RNA)


Gene name ralA
Locus tag -
Coordinates 1517725..1517903 (+)

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C3026_RS07845 1513680..1515053 + 1374 WP_000123737.1 ATP-dependent RNA helicase DbpA -
C3026_RS07850 1515182..1516117 - 936 WP_001157406.1 tRNA 2-thiocytidine(32) synthetase TtcA -
C3026_RS25695 1516169..1517404 - 1236 WP_000040852.1 site-specific integrase -
C3026_RS07860 1517406..1517621 - 216 WP_000079604.1 excisionase XisR -
C3026_RS07865 1517700..1517909 - 210 WP_000276809.1 double-strand break reduction protein RcbA -
- 1517725..1517903 + 179 NuclAT_0 - Antitoxin
- 1517725..1517903 + 179 NuclAT_0 - Antitoxin
- 1517725..1517903 + 179 NuclAT_0 - Antitoxin
- 1517725..1517903 + 179 NuclAT_0 - Antitoxin
C3026_RS07870 1517902..1518096 - 195 WP_001317028.1 type I toxin-antitoxin system endodeoxyribonuclease toxin RalR Toxin
C3026_RS07875 1518153..1518962 - 810 WP_000166319.1 recombination protein RecT -
C3026_RS07880 1518955..1521555 - 2601 WP_000105143.1 exodeoxyribonuclease VIII -
C3026_RS07885 1521657..1521932 - 276 WP_000632297.1 protein RacC -
C3026_RS07890 1522007..1522177 - 171 WP_001352098.1 conserved protein, Rac prophage -
C3026_RS07895 1522177..1522398 - 222 WP_000560225.1 killing protein KilR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Prophage - - 1515182..1537843 22661


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Endodeoxyribonuclease toxin RalR

(6-54)


Sequences

Toxin        


Download         Length: 65 a.a.        Molecular weight: 7018.89 Da        Isoelectric Point: 8.9538

>T60573 WP_001317028.1 NZ_CP014272:c1518096-1517902 [Escherichia coli K-12]
MRYDNVKPCPFCGCPSVTVKAISGYYRAKCNGCESRTGYGGSEKEALERWNKRTTGNNNGGVHV

Download         Length: 195 bp

>T60573 NZ_CP014272:c1518096-1517902 [Escherichia coli K-12]
ATGAGATATGACAATGTTAAACCATGTCCATTTTGTGGTTGTCCATCAGTAACGGTGAAAGCCATTTCAGGATATTACCG
AGCGAAGTGTAACGGATGCGAATCCCGAACCGGTTATGGTGGAAGTGAAAAAGAAGCACTCGAAAGATGGAATAAACGAA
CCACTGGAAATAATAATGGAGGTGTTCATGTATAA

Antitoxin


Download         Length: 179 bp

>AT60573 NZ_CP014272:1517725-1517903 [Escherichia coli K-12]
GAGGACTGAAGTTTCTCGCAATTAAAATTTATCAGTTTTACTTTCTGCTCTCTGGAAACGCCTGCTTCTTTTTTACCTGA
GAGCATTTTTTCGCATTCTGATTTCGTTAGTTTAGATTTTGAATATCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTT
CAATAGTGGCGGTAATTTT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7L6LHV7

Antitoxin

Download structure file

References