Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type I Classification (family/domain) ralRA/-
Location 2159123..2159494 Replicon chromosome
Accession NZ_CP010444
Organism Escherichia coli K-12 strain K-12 MG1655

Toxin (Protein)


Gene name ralR Uniprot ID F4VC37
Locus tag SH07_RS10680 Protein ID WP_001317028.1
Coordinates 2159300..2159494 (-) Length 65 a.a.

Antitoxin (RNA)


Gene name ralA
Locus tag -
Coordinates 2159123..2159301 (+)

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
SH07_RS10655 2155078..2156451 + 1374 WP_000123737.1 ATP-dependent RNA helicase DbpA -
SH07_RS10660 2156580..2157515 - 936 WP_001157406.1 tRNA 2-thiocytidine(32) synthetase TtcA -
SH07_RS23890 2157567..2158802 - 1236 WP_000040852.1 site-specific integrase -
SH07_RS10670 2158804..2159019 - 216 WP_000079604.1 excisionase XisR -
SH07_RS10675 2159098..2159307 - 210 WP_000276809.1 double-strand break reduction protein RcbA -
- 2159123..2159301 + 179 NuclAT_0 - Antitoxin
- 2159123..2159301 + 179 NuclAT_0 - Antitoxin
- 2159123..2159301 + 179 NuclAT_0 - Antitoxin
- 2159123..2159301 + 179 NuclAT_0 - Antitoxin
SH07_RS10680 2159300..2159494 - 195 WP_001317028.1 type I toxin-antitoxin system endodeoxyribonuclease toxin RalR Toxin
SH07_RS10685 2159551..2160360 - 810 WP_000166319.1 recombination protein RecT -
SH07_RS10690 2160353..2162953 - 2601 WP_000105143.1 exodeoxyribonuclease VIII -
SH07_RS10695 2163055..2163330 - 276 WP_000632297.1 protein RacC -
SH07_RS10700 2163405..2163575 - 171 WP_001352098.1 conserved protein, Rac prophage -
SH07_RS10705 2163575..2163796 - 222 WP_000560225.1 killing protein KilR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Prophage - - 2157567..2179241 21674


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Endodeoxyribonuclease toxin RalR

(6-54)


Sequences

Toxin        


Download         Length: 65 a.a.        Molecular weight: 7018.89 Da        Isoelectric Point: 8.9538

>T52666 WP_001317028.1 NZ_CP010444:c2159494-2159300 [Escherichia coli K-12]
MRYDNVKPCPFCGCPSVTVKAISGYYRAKCNGCESRTGYGGSEKEALERWNKRTTGNNNGGVHV

Download         Length: 195 bp

>T52666 NZ_CP010444:c2159494-2159300 [Escherichia coli K-12]
ATGAGATATGACAATGTTAAACCATGTCCATTTTGTGGTTGTCCATCAGTAACGGTGAAAGCCATTTCAGGATATTACCG
AGCGAAGTGTAACGGATGCGAATCCCGAACCGGTTATGGTGGAAGTGAAAAAGAAGCACTCGAAAGATGGAATAAACGAA
CCACTGGAAATAATAATGGAGGTGTTCATGTATAA

Antitoxin


Download         Length: 179 bp

>AT52666 NZ_CP010444:2159123-2159301 [Escherichia coli K-12]
GAGGACTGAAGTTTCTCGCAATTAAAATTTATCAGTTTTACTTTCTGCTCTCTGGAAACGCCTGCTTCTTTTTTACCTGA
GAGCATTTTTTCGCATTCTGATTTCGTTAGTTTAGATTTTGAATATCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTT
CAATAGTGGCGGTAATTTT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7L6LHV7

Antitoxin

Download structure file

References