Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type I Classification (family/domain) ralRA/-
Location 2169223..2169594 Replicon chromosome
Accession NZ_CP010438
Organism Escherichia coli K-12 strain K-12 MG1655

Toxin (Protein)


Gene name ralR Uniprot ID F4VC37
Locus tag SH05_RS10785 Protein ID WP_001317028.1
Coordinates 2169400..2169594 (-) Length 65 a.a.

Antitoxin (RNA)


Gene name ralA
Locus tag -
Coordinates 2169223..2169401 (+)

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
SH05_RS10760 2165178..2166551 + 1374 WP_000123737.1 ATP-dependent RNA helicase DbpA -
SH05_RS10765 2166680..2167615 - 936 WP_001157406.1 tRNA 2-thiocytidine(32) synthetase TtcA -
SH05_RS24035 2167667..2168902 - 1236 WP_000040852.1 site-specific integrase -
SH05_RS10775 2168904..2169119 - 216 WP_000079604.1 excisionase XisR -
SH05_RS10780 2169198..2169407 - 210 WP_000276809.1 double-strand break reduction protein RcbA -
- 2169223..2169401 + 179 NuclAT_0 - Antitoxin
- 2169223..2169401 + 179 NuclAT_0 - Antitoxin
- 2169223..2169401 + 179 NuclAT_0 - Antitoxin
- 2169223..2169401 + 179 NuclAT_0 - Antitoxin
SH05_RS10785 2169400..2169594 - 195 WP_001317028.1 type I toxin-antitoxin system endodeoxyribonuclease toxin RalR Toxin
SH05_RS10790 2169651..2170460 - 810 WP_000166319.1 recombination protein RecT -
SH05_RS10795 2170453..2173053 - 2601 WP_000105143.1 exodeoxyribonuclease VIII -
SH05_RS10800 2173155..2173430 - 276 WP_000632297.1 protein RacC -
SH05_RS10805 2173505..2173675 - 171 WP_001352098.1 conserved protein, Rac prophage -
SH05_RS10810 2173675..2173896 - 222 WP_000560225.1 killing protein KilR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Prophage - - 2167667..2189340 21673


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Endodeoxyribonuclease toxin RalR

(6-54)


Sequences

Toxin        


Download         Length: 65 a.a.        Molecular weight: 7018.89 Da        Isoelectric Point: 8.9538

>T52430 WP_001317028.1 NZ_CP010438:c2169594-2169400 [Escherichia coli K-12]
MRYDNVKPCPFCGCPSVTVKAISGYYRAKCNGCESRTGYGGSEKEALERWNKRTTGNNNGGVHV

Download         Length: 195 bp

>T52430 NZ_CP010438:c2169594-2169400 [Escherichia coli K-12]
ATGAGATATGACAATGTTAAACCATGTCCATTTTGTGGTTGTCCATCAGTAACGGTGAAAGCCATTTCAGGATATTACCG
AGCGAAGTGTAACGGATGCGAATCCCGAACCGGTTATGGTGGAAGTGAAAAAGAAGCACTCGAAAGATGGAATAAACGAA
CCACTGGAAATAATAATGGAGGTGTTCATGTATAA

Antitoxin


Download         Length: 179 bp

>AT52430 NZ_CP010438:2169223-2169401 [Escherichia coli K-12]
GAGGACTGAAGTTTCTCGCAATTAAAATTTATCAGTTTTACTTTCTGCTCTCTGGAAACGCCTGCTTCTTTTTTACCTGA
GAGCATTTTTTCGCATTCTGATTTCGTTAGTTTAGATTTTGAATATCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTT
CAATAGTGGCGGTAATTTT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7L6LHV7

Antitoxin

Download structure file

References