54319

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type I Classification (family/domain) ralRA/-
Location 2665694..2666065 Replicon chromosome
Accession NZ_CP011495
Organism Escherichia coli strain NCM3722 isolate K-12

Toxin (Protein)


Gene name ralR Uniprot ID F4VC37
Locus tag AA953_RS13320 Protein ID WP_001317028.1
Coordinates 2665871..2666065 (-) Length 65 a.a.

Antitoxin (RNA)


Gene name ralA
Locus tag -
Coordinates 2665694..2665872 (+)

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AA953_RS13295 2661649..2663022 + 1374 WP_000123737.1 ATP-dependent RNA helicase DbpA -
AA953_RS13300 2663151..2664086 - 936 WP_001157406.1 tRNA 2-thiocytidine(32) synthetase TtcA -
AA953_RS24840 2664138..2665373 - 1236 WP_000040852.1 site-specific integrase -
AA953_RS13310 2665375..2665590 - 216 WP_000079604.1 excisionase XisR -
AA953_RS13315 2665669..2665878 - 210 WP_000276809.1 double-strand break reduction protein RcbA -
- 2665694..2665872 + 179 NuclAT_0 - Antitoxin
- 2665694..2665872 + 179 NuclAT_0 - Antitoxin
- 2665694..2665872 + 179 NuclAT_0 - Antitoxin
- 2665694..2665872 + 179 NuclAT_0 - Antitoxin
AA953_RS13320 2665871..2666065 - 195 WP_001317028.1 type I toxin-antitoxin system endodeoxyribonuclease toxin RalR Toxin
AA953_RS13325 2666122..2666931 - 810 WP_000166319.1 recombination protein RecT -
AA953_RS13330 2666924..2669524 - 2601 WP_000105143.1 exodeoxyribonuclease VIII -
AA953_RS13335 2669626..2669901 - 276 WP_000632297.1 protein RacC -
AA953_RS13340 2669976..2670146 - 171 WP_001352098.1 conserved protein, Rac prophage -
AA953_RS13345 2670146..2670367 - 222 WP_000560225.1 killing protein KilR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Prophage - - 2664138..2685812 21674


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Endodeoxyribonuclease toxin RalR

(6-54)


Sequences

Toxin        


Download         Length: 65 a.a.        Molecular weight: 7018.89 Da        Isoelectric Point: 8.9538

>T54319 WP_001317028.1 NZ_CP011495:c2666065-2665871 [Escherichia coli]
MRYDNVKPCPFCGCPSVTVKAISGYYRAKCNGCESRTGYGGSEKEALERWNKRTTGNNNGGVHV

Download         Length: 195 bp

>T54319 NZ_CP011495:c2666065-2665871 [Escherichia coli]
ATGAGATATGACAATGTTAAACCATGTCCATTTTGTGGTTGTCCATCAGTAACGGTGAAAGCCATTTCAGGATATTACCG
AGCGAAGTGTAACGGATGCGAATCCCGAACCGGTTATGGTGGAAGTGAAAAAGAAGCACTCGAAAGATGGAATAAACGAA
CCACTGGAAATAATAATGGAGGTGTTCATGTATAA

Antitoxin


Download         Length: 179 bp

>AT54319 NZ_CP011495:2665694-2665872 [Escherichia coli]
GAGGACTGAAGTTTCTCGCAATTAAAATTTATCAGTTTTACTTTCTGCTCTCTGGAAACGCCTGCTTCTTTTTTACCTGA
GAGCATTTTTTCGCATTCTGATTTCGTTAGTTTAGATTTTGAATATCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTT
CAATAGTGGCGGTAATTTT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7L6LHV7

Antitoxin

Download structure file

References