Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type I Classification (family/domain) ralRA/-
Location 1418375..1418746 Replicon chromosome
Accession NZ_CP025268
Organism Escherichia coli str. K-12 substr. MG1655 strain K-12

Toxin (Protein)


Gene name ralR Uniprot ID F4VC37
Locus tag CXP41_RS07510 Protein ID WP_001317028.1
Coordinates 1418552..1418746 (-) Length 65 a.a.

Antitoxin (RNA)


Gene name ralA
Locus tag -
Coordinates 1418375..1418553 (+)

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CXP41_RS07485 1414330..1415703 + 1374 WP_000123737.1 ATP-dependent RNA helicase DbpA -
CXP41_RS07490 1415832..1416767 - 936 WP_001157406.1 tRNA 2-thiocytidine(32) synthetase TtcA -
CXP41_RS07495 1416819..1418054 - 1236 WP_000040852.1 site-specific integrase -
CXP41_RS07500 1418056..1418271 - 216 WP_000079604.1 excisionase XisR -
CXP41_RS07505 1418350..1418559 - 210 WP_000276809.1 double-strand break reduction protein RcbA -
- 1418375..1418553 + 179 NuclAT_0 - Antitoxin
- 1418375..1418553 + 179 NuclAT_0 - Antitoxin
- 1418375..1418553 + 179 NuclAT_0 - Antitoxin
- 1418375..1418553 + 179 NuclAT_0 - Antitoxin
CXP41_RS07510 1418552..1418746 - 195 WP_001317028.1 type I toxin-antitoxin system endodeoxyribonuclease toxin RalR Toxin
CXP41_RS07515 1418803..1419612 - 810 WP_000166319.1 recombination protein RecT -
CXP41_RS07520 1419605..1422205 - 2601 WP_000105143.1 exodeoxyribonuclease VIII -
CXP41_RS07525 1422307..1422582 - 276 WP_000632297.1 protein RacC -
CXP41_RS07530 1422657..1422827 - 171 WP_001352098.1 conserved protein, Rac prophage -
CXP41_RS07535 1422827..1423048 - 222 WP_000560225.1 killing protein KilR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Prophage - - 1416819..1438493 21674


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Endodeoxyribonuclease toxin RalR

(6-54)


Sequences

Toxin        


Download         Length: 65 a.a.        Molecular weight: 7018.89 Da        Isoelectric Point: 8.9538

>T89990 WP_001317028.1 NZ_CP025268:c1418746-1418552 [Escherichia coli str. K-12 substr. MG1655]
MRYDNVKPCPFCGCPSVTVKAISGYYRAKCNGCESRTGYGGSEKEALERWNKRTTGNNNGGVHV

Download         Length: 195 bp

>T89990 NZ_CP025268:c1418746-1418552 [Escherichia coli str. K-12 substr. MG1655]
ATGAGATATGACAATGTTAAACCATGTCCATTTTGTGGTTGTCCATCAGTAACGGTGAAAGCCATTTCAGGATATTACCG
AGCGAAGTGTAACGGATGCGAATCCCGAACCGGTTATGGTGGAAGTGAAAAAGAAGCACTCGAAAGATGGAATAAACGAA
CCACTGGAAATAATAATGGAGGTGTTCATGTATAA

Antitoxin


Download         Length: 179 bp

>AT89990 NZ_CP025268:1418375-1418553 [Escherichia coli str. K-12 substr. MG1655]
GAGGACTGAAGTTTCTCGCAATTAAAATTTATCAGTTTTACTTTCTGCTCTCTGGAAACGCCTGCTTCTTTTTTACCTGA
GAGCATTTTTTCGCATTCTGATTTCGTTAGTTTAGATTTTGAATATCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTT
CAATAGTGGCGGTAATTTT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7L6LHV7

Antitoxin

Download structure file

References