Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type I Classification (family/domain) ralRA/-
Location 1413561..1413932 Replicon chromosome
Accession NZ_CP032679
Organism Escherichia coli str. K-12 substr. MG1655 strain K-12

Toxin (Protein)


Gene name ralR Uniprot ID F4VC37
Locus tag D7U33_RS06915 Protein ID WP_001317028.1
Coordinates 1413738..1413932 (-) Length 65 a.a.

Antitoxin (RNA)


Gene name ralA
Locus tag -
Coordinates 1413561..1413739 (+)

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
D7U33_RS06890 1409516..1410889 + 1374 WP_000123737.1 ATP-dependent RNA helicase DbpA -
D7U33_RS06895 1411018..1411953 - 936 WP_001157406.1 tRNA 2-thiocytidine(32) synthetase TtcA -
D7U33_RS06900 1412005..1413240 - 1236 WP_000040852.1 site-specific integrase -
D7U33_RS06905 1413242..1413457 - 216 WP_000079604.1 excisionase XisR -
D7U33_RS06910 1413536..1413745 - 210 WP_000276809.1 double-strand break reduction protein RcbA -
- 1413561..1413739 + 179 NuclAT_0 - Antitoxin
- 1413561..1413739 + 179 NuclAT_0 - Antitoxin
- 1413561..1413739 + 179 NuclAT_0 - Antitoxin
- 1413561..1413739 + 179 NuclAT_0 - Antitoxin
D7U33_RS06915 1413738..1413932 - 195 WP_001317028.1 type I toxin-antitoxin system endodeoxyribonuclease toxin RalR Toxin
D7U33_RS06920 1413989..1414798 - 810 WP_000166319.1 recombination protein RecT -
D7U33_RS06925 1414791..1417391 - 2601 WP_000105143.1 exodeoxyribonuclease VIII -
D7U33_RS06930 1417493..1417768 - 276 WP_000632297.1 protein RacC -
D7U33_RS06935 1417843..1418013 - 171 WP_001352098.1 conserved protein, Rac prophage -
D7U33_RS06940 1418013..1418234 - 222 WP_000560225.1 killing protein KilR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Prophage - - 1412005..1433679 21674


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Endodeoxyribonuclease toxin RalR

(6-54)


Sequences

Toxin        


Download         Length: 65 a.a.        Molecular weight: 7018.89 Da        Isoelectric Point: 8.9538

>T112101 WP_001317028.1 NZ_CP032679:c1413932-1413738 [Escherichia coli str. K-12 substr. MG1655]
MRYDNVKPCPFCGCPSVTVKAISGYYRAKCNGCESRTGYGGSEKEALERWNKRTTGNNNGGVHV

Download         Length: 195 bp

>T112101 NZ_CP032679:c1413932-1413738 [Escherichia coli str. K-12 substr. MG1655]
ATGAGATATGACAATGTTAAACCATGTCCATTTTGTGGTTGTCCATCAGTAACGGTGAAAGCCATTTCAGGATATTACCG
AGCGAAGTGTAACGGATGCGAATCCCGAACCGGTTATGGTGGAAGTGAAAAAGAAGCACTCGAAAGATGGAATAAACGAA
CCACTGGAAATAATAATGGAGGTGTTCATGTATAA

Antitoxin


Download         Length: 179 bp

>AT112101 NZ_CP032679:1413561-1413739 [Escherichia coli str. K-12 substr. MG1655]
GAGGACTGAAGTTTCTCGCAATTAAAATTTATCAGTTTTACTTTCTGCTCTCTGGAAACGCCTGCTTCTTTTTTACCTGA
GAGCATTTTTTCGCATTCTGATTTCGTTAGTTTAGATTTTGAATATCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTT
CAATAGTGGCGGTAATTTT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7L6LHV7

Antitoxin

Download structure file

References