Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type I Classification (family/domain) ralRA/-
Location 1500968..1501339 Replicon chromosome
Accession NZ_CP110017
Organism Escherichia coli strain HE_50

Toxin (Protein)


Gene name ralR Uniprot ID F4VC37
Locus tag OLR76_RS07475 Protein ID WP_001317028.1
Coordinates 1501145..1501339 (-) Length 65 a.a.

Antitoxin (RNA)


Gene name ralA
Locus tag -
Coordinates 1500968..1501146 (+)

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
OLR76_RS07445 (1496720) 1496720..1496893 + 174 WP_001296046.1 protein YnaL -
OLR76_RS07450 (1496923) 1496923..1498296 + 1374 WP_000123737.1 ATP-dependent RNA helicase DbpA -
OLR76_RS07455 (1498425) 1498425..1499360 - 936 WP_001157406.1 tRNA 2-thiocytidine(32) synthetase TtcA -
OLR76_RS07460 (1499412) 1499412..1500647 - 1236 WP_000040852.1 site-specific integrase -
OLR76_RS07465 (1500649) 1500649..1500864 - 216 WP_000079604.1 excisionase XisR -
- (1500968) 1500968..1501146 + 179 NuclAT_0 - Antitoxin
- (1500968) 1500968..1501146 + 179 NuclAT_0 - Antitoxin
- (1500968) 1500968..1501146 + 179 NuclAT_0 - Antitoxin
- (1500968) 1500968..1501146 + 179 NuclAT_0 - Antitoxin
OLR76_RS07470 (1500943) 1500943..1501152 - 210 WP_000276809.1 double-strand break reduction protein RcbA -
OLR76_RS07475 (1501145) 1501145..1501339 - 195 WP_001317028.1 type I toxin-antitoxin system endodeoxyribonuclease toxin RalR Toxin
OLR76_RS07480 (1501396) 1501396..1502205 - 810 WP_000166319.1 recombination protein RecT -
OLR76_RS07485 (1502198) 1502198..1504798 - 2601 WP_000105143.1 exodeoxyribonuclease VIII -
OLR76_RS07490 (1504900) 1504900..1505175 - 276 WP_000632297.1 protein RacC -
OLR76_RS07495 (1505250) 1505250..1505420 - 171 WP_001352098.1 YdaE family protein -
OLR76_RS07500 (1505420) 1505420..1505641 - 222 WP_000560225.1 killing protein KilR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Prophage - - 1499412..1521086 21674


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Endodeoxyribonuclease toxin RalR

(6-54)


Sequences

Toxin        


Download         Length: 65 a.a.        Molecular weight: 7018.89 Da        Isoelectric Point: 8.9538

>T262714 WP_001317028.1 NZ_CP110017:c1501339-1501145 [Escherichia coli]
MRYDNVKPCPFCGCPSVTVKAISGYYRAKCNGCESRTGYGGSEKEALERWNKRTTGNNNGGVHV

Download         Length: 195 bp

Antitoxin


Download         Length: 179 bp

>AT262714 NZ_CP110017:1500968-1501146 [Escherichia coli]
GAGGACTGAAGTTTCTCGCAATTAAAATTTATCAGTTTTACTTTCTGCTCTCTGGAAACGCCTGCTTCTTTTTTACCTGA
GAGCATTTTTTCGCATTCTGATTTCGTTAGTTTAGATTTTGAATATCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTT
CAATAGTGGCGGTAATTTT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7L6LHV7

Antitoxin

Download structure file

References