1040

Detailed information of TA system    

experimentalExperimentally validated    

Overview


TA module


Type II Classification (family/domain) hipBA/Xre-HipA
Location 1588878..1590466 Replicon chromosome
Accession NC_000913
Organism Escherichia coli str. K-12 substr. MG1655

Toxin (Protein)


Gene name hipA Uniprot ID P23874
Locus tag b1507 Protein ID NP_416024.1
Coordinates 1590854..1592176 (-) Length 441 a.a.

Antitoxin (Protein)


Gene name hipB Uniprot ID P23873
Locus tag b1508 Protein ID NP_416025.1
Coordinates 1592176..1592442 (-) Length 89 a.a.

Genomic Context


Locus tag Coordinates Strand Size (bp) Protein ID Product Description
b1501 1584207..1586486 - 2280 NP_416018.1 putative oxidoreductase YdeP -
b1502 1586820..1587734 - 915 NP_416019.1 putative fimbrial adhesin protein YdeQ -
b1503 1587793..1588296 - 504 NP_416020.1 putative fimbrial protein YdeR -
b1504 1588309..1588839 - 531 NP_416021.1 putative fimbrial protein YdeS -
b1505 1588853..1590001 - 1149 YP_009518785.1 fimbrial usher domain-containing protein YdeT -
b1507 1590854..1592176 - 1323 NP_416024.1 serine/threonine-protein kinase toxin HipA Toxin
b1508 1592176..1592442 - 267 NP_416025.1 antitoxin/DNA-binding transcriptional repressor HipB Antitoxin

Associated MGEs


MGE
detail
Similar
MGEs
Relative
position
MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Genomic island - - 1580842..1592442 11600


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains


Predicted by InterproScan

Toxin

(149-401)

Antitoxin

(14-69)


Sequences


Toxin        


Download         Length: 441 a.a.        Molecular weight: 49275.82 Da        Isoelectric Point: 8.2482

>T1040 NP_416024.1 NC_000913:c1592176-1590854 [Escherichia coli str. K-12 substr. MG1655]
MPKLVTWMNNQRVGELTKLANGAHTFKYAPEWLASRYARPLSLSLPLQRGNITSDAVFNFFDNLLPDSPIVRDRIVKRYH
AKSRQPFDLLSEIGRDSVGAVTLIPEDETVTHPIMAWEKLTEARLEEVLTAYKADIPLGMIREENDFRISVAGAQEKTAL
LRIGNDWCIPKGITPTTHIIKLPIGEIRQPNATLDLSQSVDNEYYCLLLAKELGLNVPDAEIIKAGNVRALAVERFDRRW
NAERTVLLRLPQEDMCQTFGLPSSVKYESDGGPGIARIMAFLMGSSEALKDRYDFMKFQVFQWLIGATDGHAKNFSVFIQ
AGGSYRLTPFYDIISAFPVLGGTGIHISDLKLAMGLNASKGKKTAIDKIYPRHFLATAKVLRFPEVQMHEILSDFARMIP
AALDNVKTSLPTDFPENVVTAVESNVLRLHGRLSREYGSK

Download         Length: 1323 bp

>T1040 NC_000913:c1592176-1590854 [Escherichia coli str. K-12 substr. MG1655]
ATGCCTAAACTTGTCACTTGGATGAACAACCAGCGGGTAGGCGAGTTAACGAAGTTAGCCAACGGCGCGCACACCTTTAA
GTATGCACCGGAGTGGTTAGCAAGCCGTTATGCCAGACCGTTGTCACTTTCGCTGCCATTGCAGAGGGGGAATATCACCT
CTGATGCCGTATTTAACTTCTTCGATAACCTGTTACCCGATAGCCCGATTGTACGTGACCGGATCGTTAAACGTTATCAT
GCCAAATCCAGACAACCGTTTGATTTATTGTCAGAAATAGGGCGAGACAGCGTTGGTGCCGTGACGTTAATACCCGAAGA
CGAAACCGTAACGCATCCGATAATGGCATGGGAAAAGCTTACTGAAGCCAGACTTGAAGAAGTATTAACGGCTTATAAAG
CAGATATCCCGCTAGGCATGATTAGAGAAGAAAATGACTTTCGCATCTCGGTTGCTGGCGCACAGGAGAAGACAGCACTG
CTCAGAATAGGCAATGACTGGTGCATTCCGAAAGGAATAACGCCGACGACGCACATCATTAAATTACCGATTGGCGAAAT
CAGGCAGCCCAATGCGACGCTCGATCTCAGCCAAAGCGTTGATAATGAGTATTACTGTCTGCTGCTGGCGAAAGAACTTG
GGTTGAATGTTCCGGACGCAGAAATCATTAAAGCGGGAAATGTGCGCGCGTTAGCGGTCGAACGTTTTGACAGGCGTTGG
AATGCTGAGCGAACGGTTTTACTTCGCTTGCCACAGGAGGATATGTGTCAGACATTCGGTTTACCTTCATCGGTGAAATA
TGAATCAGATGGAGGCCCAGGCATCGCGCGGATCATGGCTTTTTTGATGGGGTCCAGCGAGGCGCTGAAAGATCGCTATG
ATTTTATGAAATTCCAGGTCTTCCAGTGGTTGATTGGCGCAACGGACGGTCATGCAAAAAACTTCTCCGTATTTATTCAG
GCTGGCGGCAGTTATCGACTCACGCCATTTTACGACATCATTTCAGCATTTCCGGTCCTTGGCGGTACGGGAATACACAT
CAGCGATCTCAAACTGGCAATGGGGCTTAACGCATCCAAAGGCAAAAAAACGGCAATCGATAAAATTTATCCGCGACATT
TTTTGGCGACAGCAAAGGTGCTGAGATTCCCGGAAGTGCAGATGCATGAAATCCTGAGTGACTTTGCCAGAATGATTCCA
GCAGCACTGGATAACGTGAAGACTTCATTACCGACAGATTTTCCGGAGAACGTGGTGACGGCAGTTGAAAGCAATGTGTT
GAGGTTGCATGGACGGTTAAGCCGAGAATACGGTAGTAAGTGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10016.38 Da        Isoelectric Point: 6.2267

>AT1040 NP_416025.1 NC_000913:c1592442-1592176 [Escherichia coli str. K-12 substr. MG1655]
MMSFQKIYSPTQLANAMKLVRQQNGWTQSELAKKIGIKQATISNFENNPDNTTLTTFFKILQSLELSMTLCDAKNASPES
TEQQNLEW

Download         Length: 267 bp

>AT1040 NC_000913:c1592442-1592176 [Escherichia coli str. K-12 substr. MG1655]
ATGATGAGCTTTCAGAAGATCTATAGCCCAACGCAATTGGCGAATGCAATGAAACTGGTTCGCCAGCAAAATGGCTGGAC
GCAGAGCGAGCTGGCGAAAAAAATTGGTATTAAGCAGGCGACGATTTCCAATTTCGAAAACAACCCTGACAATACCACGC
TCACGACATTTTTTAAGATTTTACAGTCGCTTGAACTCTCAATGACGCTATGCGACGCGAAAAATGCCTCGCCAGAATCA
ACAGAACAGCAAAATCTGGAGTGGTAA

Similar Proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
T10098 Escherichia coli Nissle 1917

98.157

98.861

0.97

T10106 Xenorhabdus nematophila ATCC 19061

44.495

100

0.449

T6108 Burkholderia pseudomallei K96243

39.503

100

0.398


Multiple sequence alignment    


Protein Organism Identities (%) Coverage (%) Ha-value
AT10195 Escherichia coli strain BL21 (TaKaRa)

100

100

1

AT10098 Escherichia coli Nissle 1917

95.455

100

0.955

AT10106 Xenorhabdus nematophila ATCC 19061

40.964

100

0.41


Multiple sequence alignment    




Structures


Toxin

Source ID Structure
PDB 2WIU
PDB 3DNT
PDB 3TPB
PDB 3TPT
PDB 5K98
PDB 3TPV
PDB 3DNV
PDB 3TPD
PDB 3DNU
PDB 3HZI
PDB 3FBR
PDB 4YG7
PDB 3TPE


Antitoxin

Source ID Structure
AlphaFold DB P23873

References


(1) Eitan Rotem et al. (2010) Regulation of phenotypic variability by a threshold-based mechanism underlies bacterial persistence. Proceedings of The National Academy of Sciences of The United States of America 107(28):12541-6. [PubMed:20616060] experimental

(2) Kenn Gerdes et al. (2021) Phylogeny Reveals Novel HipA-Homologous Kinase Families and Toxin-Antitoxin Gene Organizations. MBio 12(3):e0105821. [PubMed:34061596]

(3) Sonja Hansen et al. (2012) Regulation of the Escherichia coli HipBA toxin-antitoxin system by proteolysis. PloS One 7(6):e39185. [PubMed:22720069] experimental

(4) J Paul Norton et al. (2012) Toxin-antitoxin systems are important for niche-specific colonization and stress resistance of uropathogenic Escherichia coli. PLoS Pathogens 8(10):e1002954. [PubMed:23055930] experimental


experimental experimental literature