Detailed information of TA system TA246422

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-HipB
• Location: 2290509..2292097
• Replicon: chromosome
• Accession: NZ_CP097881
• Organism: Escherichia coli str. K-12 substr. MG1655 strain K-12

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: S1ELM3
• Locus tag: NAG69_RS11145
• Protein ID: WP_001125439.1
• Coordinates: 2290775..2292097 (+)
• Length: 441 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: -
• Locus tag: NAG69_RS11140
• Protein ID: WP_001301023.1
• Coordinates: 2290509..2290775 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
NAG69_RS11140 (2290509)	2290509..2290775	+	267	WP_001301023.1	type II toxin-antitoxin system antitoxin HipB	Antitoxin
NAG69_RS11145 (2290775)	2290775..2292097	+	1323	WP_001125439.1	type II toxin-antitoxin system serine/threonine protein kinase toxin HipA	Toxin
NAG69_RS11150 (2292409)	2292409..2292617	+	209	Protein_2178	AraC family transcriptional regulator	-
NAG69_RS11155 (2292872)	2292872..2294098	+	1227	Protein_2179	fimbria/pilus outer membrane usher protein	-
NAG69_RS11160 (2294112)	2294112..2294642	+	531	WP_000876763.1	fimbrial protein	-
NAG69_RS11165 (2294655)	2294655..2295158	+	504	WP_000825452.1	fimbrial protein	-
NAG69_RS11170 (2295217)	2295217..2296131	+	915	WP_000520676.1	fimbrial protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	Similar MGE(s) Similar MGE(s) is defined as other TA-related MGE(s) with Mash distance < 0.01 to this MGE. Detail Genome accession Organism MGE type Related TA Coordinates Mash distance View CP009789 Escherichia coli K-12 strain ER3413 Genomic island TA020025 1561271..1572871 0.003 View HG738867 Escherichia coli str. K-12 substr. MC4100 Genomic island TA020066 1469371..1480971 0.003 View NC_000913 Escherichia coli str. K-12 substr. MG1655 Genomic island TA001040 1580842..1592442 0.003 View NC_007779 Escherichia coli str. K-12 substr. W3110 Genomic island TA021437 1582556..1594156 0.003 View NC_009800 Escherichia coli HS Genomic island TA022421 1597665..1609265 0.005 View NC_010473 Escherichia coli str. K-12 substr. DH10B Genomic island TA022685 1669461..1680091 0.005 View NC_012759 Escherichia coli BW2952 Genomic island TA023853 1470925..1482525 0.003 View NC_017638 Escherichia coli DH1 Genomic island TA027403 1563024..1574624 0.003 View NC_020518 Escherichia coli str. K-12 substr. MDS42 Genomic island TA029002 1287669..1299269 0.003 View NZ_CP009273 Escherichia coli BW25113 Genomic island TA048682 1575099..1586699 0.003 View NZ_CP017979 Escherichia coli str. K-12 substr. W3110 strain K-12 substr. ZK126 Genomic island TA068424 1484612..1496212 0.003 View NZ_CP025520 Escherichia coli strain DH5alpha Genomic island TA090478 438284..449884 0.003 View NZ_HG738867 Escherichia coli str. K-12 substr. MC4100 strain K-12 Genomic island TA285016 1469371..1480971 0.003	inside	Genomic island	-	-	2290509..2303318	12809

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA-like, C-terminal (149-401)

Antitoxin

• Cro/C1-type helix-turn-helix domain (14-69)

Sequences

Toxin

Protein

• Length: 441 a.a.
• Molecular weight: 49275.82 Da
• Isoelectric Point: 8.2482

>T246422 WP_001125439.1 NZ_CP097881:2290775-2292097 [Escherichia coli str. K-12 substr. MG1655]
MPKLVTWMNNQRVGELTKLANGAHTFKYAPEWLASRYARPLSLSLPLQRGNITSDAVFNFFDNLLPDSPIVRDRIVKRYH
AKSRQPFDLLSEIGRDSVGAVTLIPEDETVTHPIMAWEKLTEARLEEVLTAYKADIPLGMIREENDFRISVAGAQEKTAL
LRIGNDWCIPKGITPTTHIIKLPIGEIRQPNATLDLSQSVDNEYYCLLLAKELGLNVPDAEIIKAGNVRALAVERFDRRW
NAERTVLLRLPQEDMCQTFGLPSSVKYESDGGPGIARIMAFLMGSSEALKDRYDFMKFQVFQWLIGATDGHAKNFSVFIQ
AGGSYRLTPFYDIISAFPVLGGTGIHISDLKLAMGLNASKGKKTAIDKIYPRHFLATAKVLRFPEVQMHEILSDFARMIP
AALDNVKTSLPTDFPENVVTAVESNVLRLHGRLSREYGSK

Nucleotide

• Length: 1323 bp

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10016.38 Da
• Isoelectric Point: 6.2267

>AT246422 WP_001301023.1 NZ_CP097881:2290509-2290775 [Escherichia coli str. K-12 substr. MG1655]
MMSFQKIYSPTQLANAMKLVRQQNGWTQSELAKKIGIKQATISNFENNPDNTTLTTFFKILQSLELSMTLCDAKNASPES
TEQQNLEW

Nucleotide

• Length: 267 bp

Structures

Toxin

• PDB: 5K98
• PDB: 3TPV
• PDB: 3DNV
• PDB: 3TPD
• PDB: 3HZI
• PDB: 3FBR
• PDB: 4YG7
• PDB: 3TPE
• PDB: 2WIU
• PDB: 3DNT
• PDB: 3TPB
• PDB: 3TPT
• PDB: 3DNU