Detailed information of TA system TA157101

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-HipB
• Location: 1681121..1682709
• Replicon: chromosome
• Accession: NZ_CP053604
• Organism: Escherichia coli strain NEB10-beta

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: S1ELM3
• Locus tag: HO394_RS08170
• Protein ID: WP_001125439.1
• Coordinates: 1681121..1682443 (-)
• Length: 441 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: -
• Locus tag: HO394_RS08175
• Protein ID: WP_001301023.1
• Coordinates: 1682443..1682709 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HO394_RS08145	1678057..1678971	-	915	WP_000520676.1	fimbrial protein	-
HO394_RS08150	1679030..1679533	-	504	WP_000825452.1	type 1 fimbrial protein	-
HO394_RS08155	1679546..1679992	-	447	Protein_1593	type 1 fimbrial protein	-
HO394_RS08160	1679993..1680346	-	354	Protein_1594	fimbria/pilus outer membrane usher protein	-
HO394_RS08165	1680601..1680809	-	209	Protein_1595	helix-turn-helix transcriptional regulator	-
HO394_RS08170	1681121..1682443	-	1323	WP_001125439.1	type II toxin-antitoxin system serine/threonine protein kinase toxin HipA	Toxin
HO394_RS08175	1682443..1682709	-	267	WP_001301023.1	type II toxin-antitoxin system antitoxin HipB	Antitoxin
HO394_RS08180	1682932..1684332	-	1401	WP_001083595.1	autotransporter outer membrane beta-barrel domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	Similar MGE(s) Similar MGE(s) is defined as other TA-related MGE(s) with Mash distance < 0.01 to this MGE. Detail Genome accession Organism MGE type Related TA Coordinates Mash distance View CP009789 Escherichia coli K-12 strain ER3413 Genomic island TA020025 1561271..1572871 0.002 View HG738867 Escherichia coli str. K-12 substr. MC4100 Genomic island TA020066 1469371..1480971 0.002 View NC_000913 Escherichia coli str. K-12 substr. MG1655 Genomic island TA001040 1580842..1592442 0.002 View NC_007779 Escherichia coli str. K-12 substr. W3110 Genomic island TA021437 1582556..1594156 0.002 View NC_009800 Escherichia coli HS Genomic island TA022421 1597665..1609265 0.005 View NC_010473 Escherichia coli str. K-12 substr. DH10B Genomic island TA022685 1669461..1680091 0 View NC_012759 Escherichia coli BW2952 Genomic island TA023853 1470925..1482525 0.002 View NC_017638 Escherichia coli DH1 Genomic island TA027403 1563024..1574624 0.002 View NC_020518 Escherichia coli str. K-12 substr. MDS42 Genomic island TA029002 1287669..1299269 0.002 View NZ_CP009273 Escherichia coli BW25113 Genomic island TA048682 1575099..1586699 0.002 View NZ_CP017979 Escherichia coli str. K-12 substr. W3110 strain K-12 substr. ZK126 Genomic island TA068424 1484612..1496212 0.002 View NZ_CP025520 Escherichia coli strain DH5alpha Genomic island TA090478 438284..449884 0.002 View NZ_HG738867 Escherichia coli str. K-12 substr. MC4100 strain K-12 Genomic island TA285016 1469371..1480971 0.002	inside	Genomic island	-	-	1672079..1682709	10630

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA-like, C-terminal (149-401)

Antitoxin

• Cro/C1-type helix-turn-helix domain (14-69)

Sequences

Toxin

Protein

• Length: 441 a.a.
• Molecular weight: 49275.82 Da
• Isoelectric Point: 8.2482

>T157101 WP_001125439.1 NZ_CP053604:c1682443-1681121 [Escherichia coli]
MPKLVTWMNNQRVGELTKLANGAHTFKYAPEWLASRYARPLSLSLPLQRGNITSDAVFNFFDNLLPDSPIVRDRIVKRYH
AKSRQPFDLLSEIGRDSVGAVTLIPEDETVTHPIMAWEKLTEARLEEVLTAYKADIPLGMIREENDFRISVAGAQEKTAL
LRIGNDWCIPKGITPTTHIIKLPIGEIRQPNATLDLSQSVDNEYYCLLLAKELGLNVPDAEIIKAGNVRALAVERFDRRW
NAERTVLLRLPQEDMCQTFGLPSSVKYESDGGPGIARIMAFLMGSSEALKDRYDFMKFQVFQWLIGATDGHAKNFSVFIQ
AGGSYRLTPFYDIISAFPVLGGTGIHISDLKLAMGLNASKGKKTAIDKIYPRHFLATAKVLRFPEVQMHEILSDFARMIP
AALDNVKTSLPTDFPENVVTAVESNVLRLHGRLSREYGSK

Nucleotide

• Length: 1323 bp

>T157101 NZ_CP069076:c2183552-2183223 [Mycobacterium tuberculosis]
GTGACCGCACTTCCGGCGCGCGGAGAGGTGTGGTGGTGTGAGATGGCTGAGATCGGTCGGCGACCAGTCGTCGTGCTGTC
GCGCGATGCCGCGATCCCTCGGCTGCGACGCGCACTTGTCGCGCCCTGCACCACGACCATCCGAGGGCTAGCCAGTGAGG
TTGTTCTTGAACCCGGTTCCGACCCGATCCCGCGCCGTTCCGCGGTGAATTTGGACTCAGTCGAAAGTGTCTCGGTCGCG
GTATTGGTGAATCGGCTTGGCCGCCTCGCCGACATCCGGATGCGCGCCATCTGCACGGCCCTCGAGGTCGCCGTCGATTG
CTCTCGATGA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10016.38 Da
• Isoelectric Point: 6.2267

>AT157101 WP_001301023.1 NZ_CP053604:c1682709-1682443 [Escherichia coli]
MMSFQKIYSPTQLANAMKLVRQQNGWTQSELAKKIGIKQATISNFENNPDNTTLTTFFKILQSLELSMTLCDAKNASPES
TEQQNLEW

Nucleotide

• Length: 267 bp

>AT157101 NZ_CP069076:c2183926-2183549 [Mycobacterium tuberculosis]
GTGAAGACGGCCCGGTTGCAGGTGACGTTGCGCTGCGCGGTAGACCTCATCAATTCGTCCAGCGACCAATGTTTCGCCCG
TATCGAGCACGTTGCGTCCGATCAGGCGGATCCACGCCCCGGAGTGTGGCATAGTAGTGGCATGAATCGCATACGGCTGA
GCACTACTGTCGACGCCGCACTCTTGACAAGTGCGCGCGACATGCGGGCAGGAATCACTGACGCAGCCCTCATCGATGAG
GCCCTTGCGGCATTACTGGCTCGTCATCGATCGGCCGAGGTGGATGCCAGCTATGCGGCCTACGACAAGCACCCGGTCGA
TGAGCCCGACGAGTGGGGCGATCTAGCGTCGTGGCGGCGGGCGGCTGGAGACTCGTGA

Structures

Toxin

• PDB: 5K98
• PDB: 3TPV
• PDB: 3DNV
• PDB: 3TPD
• PDB: 3HZI
• PDB: 3FBR
• PDB: 4YG7
• PDB: 3TPE
• PDB: 2WIU
• PDB: 3DNT
• PDB: 3TPB
• PDB: 3TPT
• PDB: 3DNU