Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hipBA/HipA-HipB
Location 2983612..2985200 Replicon chromosome
Accession NZ_CP083274
Organism Escherichia coli strain Rosetta2-DE3

Toxin (Protein)


Gene name hipA Uniprot ID S1ELM3
Locus tag K6U01_RS14320 Protein ID WP_001125439.1
Coordinates 2983612..2984934 (-) Length 441 a.a.

Antitoxin (Protein)


Gene name hipB Uniprot ID -
Locus tag K6U01_RS14325 Protein ID WP_001301023.1
Coordinates 2984934..2985200 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K6U01_RS14305 (2978995) 2978995..2981645 - 2651 Protein_2819 fimbrial biogenesis outer membrane usher protein -
K6U01_RS14310 (2981687) 2981687..2982397 - 711 Protein_2820 fimbria/pilus periplasmic chaperone -
K6U01_RS14315 (2982758) 2982758..2983240 - 483 WP_000931227.1 fimbrial protein -
K6U01_RS14320 (2983612) 2983612..2984934 - 1323 WP_001125439.1 type II toxin-antitoxin system serine/threonine protein kinase toxin HipA Toxin
K6U01_RS14325 (2984934) 2984934..2985200 - 267 WP_001301023.1 type II toxin-antitoxin system antitoxin HipB Antitoxin
K6U01_RS14330 (2985423) 2985423..2987003 - 1581 Protein_2824 autotransporter outer membrane beta-barrel domain-containing protein -
K6U01_RS14335 (2986999) 2986999..2987775 + 777 Protein_2825 histidine kinase -
K6U01_RS14340 (2987787) 2987787..2988809 + 1023 WP_000172488.1 autoinducer 2 ABC transporter substrate-binding protein LsrB -
K6U01_RS14345 (2988836) 2988836..2989711 + 876 WP_000774188.1 3-hydroxy-5-phosphonooxypentane-2,4-dione thiolase -
K6U01_RS14350 (2989735) 2989735..2990025 + 291 WP_000558527.1 (4S)-4-hydroxy-5-phosphonooxypentane-2,3-dione isomerase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Genomic island - fimC 2970984..2985200 14216


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HipA-like, C-terminal

(149-401)

Antitoxin

Cro/C1-type helix-turn-helix domain

(14-69)


Sequences

Toxin        


Download         Length: 441 a.a.        Molecular weight: 49275.82 Da        Isoelectric Point: 8.2482

>T216276 WP_001125439.1 NZ_CP083274:c2984934-2983612 [Escherichia coli]
MPKLVTWMNNQRVGELTKLANGAHTFKYAPEWLASRYARPLSLSLPLQRGNITSDAVFNFFDNLLPDSPIVRDRIVKRYH
AKSRQPFDLLSEIGRDSVGAVTLIPEDETVTHPIMAWEKLTEARLEEVLTAYKADIPLGMIREENDFRISVAGAQEKTAL
LRIGNDWCIPKGITPTTHIIKLPIGEIRQPNATLDLSQSVDNEYYCLLLAKELGLNVPDAEIIKAGNVRALAVERFDRRW
NAERTVLLRLPQEDMCQTFGLPSSVKYESDGGPGIARIMAFLMGSSEALKDRYDFMKFQVFQWLIGATDGHAKNFSVFIQ
AGGSYRLTPFYDIISAFPVLGGTGIHISDLKLAMGLNASKGKKTAIDKIYPRHFLATAKVLRFPEVQMHEILSDFARMIP
AALDNVKTSLPTDFPENVVTAVESNVLRLHGRLSREYGSK

Download         Length: 1323 bp

>T216276 NZ_CP112997:c3096479-3096087 [Mycobacterium tuberculosis variant bovis]
ATGACGACGGTGCTGCTCGACTCGCATGTGGCCTACTGGTGGTCGGCCGAGCCGCAGCGTCTCAGCATGGCGGCGAGCCA
GGCCATCGAACACGCCGACGAGCTCGCCGTCGCCGCGATTTCGTGGTTCGAGCTGGCTTGGCTTGCCGAACAGGAACGCA
TCCAACTGGCGATTCCGGTGCTGTCCTGGCTTCAGCAGCTGGCCGAGCACGTTCGCACCGTCGGTATCACGCCCTCGGTC
GCCGCCACGGCGGTGGCGCTGCCCTCGTCGTTCCCCGGCGATCCGGCCGACCGGTTGATCTACGCCACCGCGATCGAACA
CGGCTGGCGGCTGGTGACCAAGGACCGGCGGCTACGCAGTCATCGGCACCCACGACCGGTCACCGTCTGGTAG

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10016.38 Da        Isoelectric Point: 6.2267

>AT216276 WP_001301023.1 NZ_CP083274:c2985200-2984934 [Escherichia coli]
MMSFQKIYSPTQLANAMKLVRQQNGWTQSELAKKIGIKQATISNFENNPDNTTLTTFFKILQSLELSMTLCDAKNASPES
TEQQNLEW

Download         Length: 267 bp

>AT216276 NZ_CP112997:c3096691-3096476 [Mycobacterium tuberculosis variant bovis]
ATGACCGCTACGGAGGTGAAGGCGAAGATCCTCTCCTTGCTTGATGAAGTGGCCCAGGGCGAGGAGATCGAGATCACCAA
ACACGGCCGCACCGTGGCCCGGCTGGTGGCAGCGACGGGGCCGCACGCGCTGAAGGGTCGATTCTCGGGTGTGGCGATGG
CGGCCGTGGATGACGACGAACTCTTCACCACCGGGGTTTCGTGGAACGTTTCATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 5K98
PDB 3TPV
PDB 3DNV
PDB 3TPD
PDB 3HZI
PDB 3FBR
PDB 4YG7
PDB 3TPE
PDB 2WIU
PDB 3DNT
PDB 3TPB
PDB 3TPT
PDB 3DNU

Antitoxin

Source ID Structure

References