10098

Detailed information of TA system    

experimentalExperimentally validated    

Overview

TA module


Type II Classification (family/domain) hipBA/-
Location 1709929..1711514 Replicon chromosome
Accession NZ_CP007799
Organism Escherichia coli Nissle 1917

Toxin (Protein)


Gene name hipA Uniprot ID -
Locus tag ECOLIN_RS08665 Protein ID WP_038427096.1
Coordinates 1709929..1711248 (-) Length 440 a.a.

Antitoxin (Protein)


Gene name hipB Uniprot ID V0VAH2
Locus tag ECOLIN_RS08670 Protein ID WP_001304368.1
Coordinates 1711248..1711514 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ECOLIN_RS08635 (ECOLIN_08340) 1705282..1705992 - 711 WP_001195181.1 fimbria/pilus periplasmic chaperone -
ECOLIN_RS08640 (ECOLIN_08345) 1706354..1706917 - 564 WP_001327671.1 type 1 fimbrial major subunit FimA -
ECOLIN_RS08650 (ECOLIN_08355) 1707693..1708475 - 783 WP_001221834.1 SDR family oxidoreductase -
ECOLIN_RS32590 1708624..1709205 + 582 Protein_1649 LysR family transcriptional regulator -
ECOLIN_RS32595 1709253..1709666 + 414 WP_000753076.1 acetylxylan esterase -
ECOLIN_RS08665 (ECOLIN_08365) 1709929..1711248 - 1320 WP_038427096.1 type II toxin-antitoxin system serine/threonine protein kinase toxin HipA Toxin
ECOLIN_RS08670 (ECOLIN_08370) 1711248..1711514 - 267 WP_001304368.1 type II toxin-antitoxin system antitoxin HipB Antitoxin
ECOLIN_RS08675 (ECOLIN_08375) 1711822..1712580 + 759 WP_001286540.1 trans-aconitate 2-methyltransferase -
ECOLIN_RS08680 (ECOLIN_08380) 1712584..1713498 - 915 WP_001443133.1 bestrophin family protein -
ECOLIN_RS08690 (ECOLIN_08385) 1713687..1715147 - 1461 WP_000854661.1 tagaturonate reductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Genomic island - fimA 1694571..1711514 16943


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HipA, N-terminal subdomain 1

(4-103)

Antitoxin

Cro/C1-type helix-turn-helix domain

(14-69)


Sequences

Toxin        


Download         Length: 440 a.a.        Molecular weight: 49248.98 Da        Isoelectric Point: 8.9411

>T10098 WP_038427096.1 NZ_CP007799:c1711248-1709929 [Escherichia coli Nissle 1917]
MPKLVTWMNNQRVGELTKLANGAHTFKYAPEWLASRYARPLSLSLPLQRGNITSDAVFNFFDNLLPDSPIVRDRIVKRYH
AKSRQPFDLLSEIGRDSVGAVTLIPEDETVTCPIMAWEKLTEARLEEVLTAYKADIPLGMIREENDFRISVAGAQEKTAL
LRIGNDWCIPKGITPTTHIIKLPIGEIRQPNATLDLSQSVDNEYYCLLLAKELGLNVPDAEIIKAGRVRALAVKRFDRRW
NTERTVLLRLPQEDMCQTFGLPSSVKYESDGGPGIAQIMAFLMGSSEALKDRYDFMKFQVFQWLIGATDGHAKNFSVFIQ
AGGSYRLTPFYDIISAFPVLGGTGIHISDLKLAMGLNASKGKKTAIDKIYPRHFLATAKVLKFPEVQMHEILSDFARMIP
AALDNVKNSLPTDFPENVVTAVETNVLRLHGRLSPRIRY

Download         Length: 1320 bp

>T10098 NZ_CP007799:c1711248-1709929 [Escherichia coli Nissle 1917]
ATGCCTAAACTAGTCACTTGGATGAACAACCAGCGGGTAGGCGAGTTAACGAAGTTAGCCAACGGCGCGCACACCTTTAA
GTATGCACCTGAGTGGTTAGCCAGCCGTTATGCCAGACCGTTGTCACTTTCGCTGCCATTGCAGAGGGGGAATATCACCT
CTGATGCCGTATTTAACTTCTTCGATAACCTGTTACCCGATAGCCCGATTGTACGTGACCGGATCGTTAAACGTTATCAT
GCTAAATCCAGACAACCGTTTGATTTATTGTCAGAAATAGGGCGAGATAGCGTTGGTGCCGTGACGTTAATCCCCGAAGA
TGAAACCGTAACGTGTCCGATAATGGCATGGGAAAAGCTTACTGAAGCCAGACTTGAAGAAGTATTAACGGCTTATAAAG
CAGATATCCCGTTAGGCATGATTAGAGAAGAAAATGACTTTCGCATCTCGGTTGCTGGCGCACAGGAGAAGACTGCGCTG
CTCAGAATAGGCAATGACTGGTGCATTCCGAAGGGAATAACGCCAACGACGCACATTATCAAATTGCCGATTGGCGAAAT
CAGACAGCCCAATGCGACGCTCGATCTTAGCCAAAGCGTTGATAATGAGTATTACTGTTTGCTGCTGGCGAAAGAACTCG
GGTTGAATGTTCCGGACGCAGAAATCATTAAAGCGGGAAGGGTGCGCGCGTTAGCGGTCAAACGTTTTGACAGGCGTTGG
AATACTGAGCGAACGGTTTTACTTCGCTTGCCACAGGAGGATATGTGCCAGACATTCGGTTTACCTTCATCGGTGAAATA
TGAATCAGATGGAGGCCCCGGTATCGCGCAAATTATGGCTTTTTTGATGGGGTCCAGCGAGGCGCTGAAAGATCGCTATG
ATTTTATGAAGTTTCAGGTCTTCCAGTGGTTGATTGGCGCAACAGATGGCCATGCAAAAAACTTCTCCGTATTTATTCAG
GCTGGCGGCAGTTATCGGCTCACACCATTTTATGACATCATTTCGGCATTTCCGGTCCTTGGCGGTACGGGAATACACAT
CAGCGATCTCAAACTGGCAATGGGGCTTAACGCATCCAAAGGCAAAAAAACGGCAATCGATAAAATTTATCCGCGACATT
TTCTGGCGACAGCAAAGGTGCTGAAATTCCCGGAAGTGCAGATGCATGAAATCCTGAGTGACTTTGCCAGAATGATTCCG
GCAGCACTGGATAACGTGAAGAATTCATTACCGACAGATTTTCCGGAGAACGTGGTGACGGCAGTTGAAACCAATGTGTT
GAGGTTGCACGGTCGGTTAAGCCCCAGAATACGGTATTAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9957.31 Da        Isoelectric Point: 4.9632

>AT10098 WP_001304368.1 NZ_CP007799:c1711514-1711248 [Escherichia coli Nissle 1917]
MMSFQKIYSPTQLANAMKLVRQQNGWTQSELAKKIGIKQATISNFENNPDNTSLTTFFKILQSLELSMTLCDAKNASPEA
AEQQDLEW

Download         Length: 267 bp

>AT10098 NZ_CP007799:c1711514-1711248 [Escherichia coli Nissle 1917]
ATGATGAGCTTTCAGAAGATCTATAGCCCAACGCAATTGGCGAATGCAATGAAACTGGTTCGCCAGCAAAACGGCTGGAC
GCAGAGCGAGTTGGCGAAAAAAATCGGCATTAAGCAGGCGACAATTTCCAATTTCGAAAACAATCCTGACAATACCTCGC
TCACGACATTTTTTAAGATTTTACAGTCGCTTGAACTCTCAATGACGCTATGCGACGCGAAAAATGCCTCGCCAGAAGCA
GCAGAACAGCAAGATTTGGAGTGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
T1040 Escherichia coli str. K-12 substr. MG1655

98.157

98.861

0.97
T10106 Xenorhabdus nematophila ATCC 19061

44.828

100

0.451
T6108 Burkholderia pseudomallei K96243

39.588

99.544

0.394

Multiple sequence alignment    


Protein Organism Identities (%) Coverage (%) Ha-value
AT10195 Escherichia coli strain BL21 (TaKaRa)

95.455

100

0.955
AT1040 Escherichia coli str. K-12 substr. MG1655

95.455

100

0.955
AT10106 Xenorhabdus nematophila ATCC 19061

39.759

100

0.398

Multiple sequence alignment    




Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure
AlphaFold DB V0VAH2

References

(1) Jun Xu et al. (2020) Functional investigation of the chromosomal ccdAB and hipAB operon in Escherichia coli Nissle 1917. Applied Microbiology And Biotechnology 104(15):6731-6747. [PubMed:32535695] experimental


experimental experimental literature