215002

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hipBA/HipA-HipB
Location 2610290..2611878 Replicon chromosome
Accession NZ_CP082777
Organism Escherichia coli O25:H1 strain CU39ES-1

Toxin (Protein)


Gene name hipA Uniprot ID A0A1L1KDK2
Locus tag K8B91_RS12760 Protein ID WP_001125431.1
Coordinates 2610290..2611612 (-) Length 441 a.a.

Antitoxin (Protein)


Gene name hipB Uniprot ID V0VAH2
Locus tag K8B91_RS12765 Protein ID WP_001304368.1
Coordinates 2611612..2611878 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K8B91_RS12730 (2605647) 2605647..2606357 - 711 WP_001195181.1 fimbria/pilus periplasmic chaperone -
K8B91_RS12735 (2606719) 2606719..2607282 - 564 WP_001327671.1 type 1 fimbrial major subunit FimA -
K8B91_RS12740 (2607707) 2607707..2607814 + 108 Protein_2478 hypothetical protein -
K8B91_RS12745 (2608058) 2608058..2608840 - 783 WP_001221834.1 SDR family oxidoreductase -
K8B91_RS12750 (2608989) 2608989..2609570 + 582 Protein_2480 LysR family transcriptional regulator -
K8B91_RS12755 (2609573) 2609573..2609980 + 408 Protein_2481 acetylxylan esterase -
K8B91_RS12760 (2610290) 2610290..2611612 - 1323 WP_001125431.1 type II toxin-antitoxin system serine/threonine protein kinase toxin HipA Toxin
K8B91_RS12765 (2611612) 2611612..2611878 - 267 WP_001304368.1 type II toxin-antitoxin system antitoxin HipB Antitoxin
K8B91_RS12770 (2612186) 2612186..2612944 + 759 WP_001286540.1 trans-aconitate 2-methyltransferase -
K8B91_RS12775 (2612948) 2612948..2613862 - 915 WP_001443133.1 bestrophin family protein -
K8B91_RS12780 (2614059) 2614059..2615510 - 1452 WP_000854660.1 tagaturonate reductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Genomic island - fimA 2594936..2611878 16942


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HipA, N-terminal subdomain 1

(4-103)

Antitoxin

Cro/C1-type helix-turn-helix domain

(14-69)


Sequences

Toxin        


Download         Length: 441 a.a.        Molecular weight: 49310.02 Da        Isoelectric Point: 8.6972

>T215002 WP_001125431.1 NZ_CP082777:c2611612-2610290 [Escherichia coli O25:H1]
MPKLVTWMNNQRVGELTKLANGAHTFKYAPEWLASRYARPLSLSLPLQRGNITSDAVFNFFDNLLPDSPIVRDRIVKRYH
AKSRQPFDLLSEIGRDSVGAVTLIPEDETVTCPIMAWEKLTEARLEEVLTAYKADIPLGMIREENDFRISVAGAQEKTAL
LRIGNDWCIPKGITPTTHIIKLPIGEIRQPNATLDLSQSVDNEYYCLLLAKELGLNVPDAEIIKAGRVRALAVKRFDRRW
NTERTVLLRLPQEDMCQTFGLPSSVKYESDGGPGIAQIMAFLMGSSEALKDRYDFMKFQVFQWLIGATDGHAKNFSVFIQ
AGGSYRLTPFYDIISAFPVLGGTGIHISDLKLAMGLNASKGKKTAIDKIYPRHFLATAKVLKFPEVQMHEILSDFARMIP
AALDNVKNSLPTDFPENVVTAVETNVLRLHGRLSREYGIK

Download         Length: 1323 bp

>T215002 NZ_CP110362:c490413-490099 [Dickeya dadantii]
ATGCAATTCACCGTATACGGTAATACCGGGAAAAGCGCCGTTTATCCGTTGTTGCTCGATGTCACGAGCGATATTATTGG
ACAGTTGAATCGTCGGATAGTGATCCCGTTACTCCCTGTTGAAAAGTATCCGGCAGGCCGCCGACCGGAGCGCTTTGTCC
CCGTCGTCAGGCTGACGGACGGCAAAGAATACGCCGTAATGACGCACGAGCTGGCAAGTATTCCTGTGCAGGCTCTGGGG
ACTGTGTTTTGTGATGCGTCGCAGTACCGTAATCAGATAAAGGCCGCAATAGACTTCCTCATCGACGGCATTTAG

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9957.31 Da        Isoelectric Point: 4.9632

>AT215002 WP_001304368.1 NZ_CP082777:c2611878-2611612 [Escherichia coli O25:H1]
MMSFQKIYSPTQLANAMKLVRQQNGWTQSELAKKIGIKQATISNFENNPDNTSLTTFFKILQSLELSMTLCDAKNASPEA
AEQQDLEW

Download         Length: 267 bp

>AT215002 NZ_CP110362:c490652-490416 [Dickeya dadantii]
ATGACCGCGAAACAACGCAACACGCAGAGCGTGACCATGACGGTAGAGCGAGCCTTACTGGTCCGGGCGCGTGAGGCGGG
TATTAACCTGAGTGCTACCCTGTCAACCGCACTGGATGCAGAGCTTCGTCACCATGAAGCGAAAAAATGGCAGGAAGAGA
ACAGGGAGGCCATCGACGCATTAAATCGTTTTCATGATGAACACGGCTGTTTCAGTGATGAATACCGGACGTTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1L1KDK2

Antitoxin

Source ID Structure
AlphaFold DB V0VAH2

References