Detailed information of TA system TA141428

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-HipB
• Location: 2359640..2361228
• Replicon: chromosome
• Accession: NZ_CP045206
• Organism: Escherichia coli strain DE17

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: B7MMY1
• Locus tag: GBG22_RS11365
• Protein ID: WP_001125475.1
• Coordinates: 2359640..2360962 (-)
• Length: 441 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: -
• Locus tag: GBG22_RS11370
• Protein ID: WP_001332145.1
• Coordinates: 2360962..2361228 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GBG22_RS11345	2355821..2356531	-	711	WP_001195181.1	fimbria/pilus periplasmic chaperone	-
GBG22_RS11350	2356893..2357456	-	564	WP_039267869.1	type 1 fimbrial major subunit FimA	-
GBG22_RS11355	2358232..2359014	-	783	WP_001221834.1	SDR family oxidoreductase	-
GBG22_RS11360	2359124..2359381	+	258	WP_001210030.1	hypothetical protein	-
GBG22_RS11365	2359640..2360962	-	1323	WP_001125475.1	type II toxin-antitoxin system serine/threonine protein kinase toxin HipA	Toxin
GBG22_RS11370	2360962..2361228	-	267	WP_001332145.1	type II toxin-antitoxin system antitoxin HipB	Antitoxin
GBG22_RS11375	2361536..2362294	+	759	WP_001286588.1	trans-aconitate 2-methyltransferase	-
GBG22_RS11380	2362298..2363212	-	915	WP_001306069.1	bestrophin family protein	-
GBG22_RS11385	2363409..2364860	-	1452	WP_000854637.1	tagaturonate reductase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	Similar MGE(s) Similar MGE(s) is defined as other TA-related MGE(s) with Mash distance < 0.01 to this MGE. Detail Genome accession Organism MGE type Related TA Coordinates Mash distance View NZ_CP007799 Escherichia coli Nissle 1917 Genomic island TA010098 1694571..1711514 0.007	inside	Genomic island	-	fimA	2345111..2361228	16117

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA-like, C-terminal (149-401)

Antitoxin

• Cro/C1-type helix-turn-helix domain (18-69)

Sequences

Toxin

Protein

• Length: 441 a.a.
• Molecular weight: 49426.24 Da
• Isoelectric Point: 8.9380

>T141428 WP_001125475.1 NZ_CP045206:c2360962-2359640 [Escherichia coli]
MPKLVTWMNNQRVGELTKLANGAHTFKYAPEWLASRYARPLSLSLPLQRRNITSDAVFNFFDNLLPDSPIVRDRIVKRYH
AKSRQPFDLLSEIGRDSVGAVTLIPEDETVMCPIMAWEKLTEARLEEVLTAYKADIPLGMIREENDFRISVAGAQEKTAL
LRIGNDWCIPKGITPTTHIIKLPIGEIRQPNATLDLSQSVDNEYYCLLLAKELGLNVPDAEIIKAGRVRALAVKRFDRRW
NTERTVLLRLPQEDMCQTFGLPSSVKYESDGGPGIAQIMAFLMGSSEALKDRYDFMKFQVFQWLIGATDGHAKNFSVFIQ
AGGSYRLTPFYDIISAFPVLGGTGIHISDLKLAMGLNASKGKKTAIDKIYPRHFLATAKVLKFPEVQMHEILSDFARMIP
AALDNVKTSLPTDFPENVVTAVETNVLRLHGRLSREYGIK

Nucleotide

• Length: 1323 bp

>T141428 NZ_CP059960:1069618-1069797 [Pectobacterium brasiliense]
ATGGATAGCAGGACGTTAATAGCAGAAATCAAGGCCGATGGGTGGGAACTGATAAGAGTGAACGGAAGCCACCACCATTT
TATGCATCCTAGTAAACCGGGGTTAGTCACAATCCCCCACCCTAAGAAAGATTTACCGATAGGCACGGTAAAAAGCATCA
GGAAACAAGCAGGAATATGA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10016.44 Da
• Isoelectric Point: 6.2267

>AT141428 WP_001332145.1 NZ_CP045206:c2361228-2360962 [Escherichia coli]
MMSFQKIYSPMQLANAMKLVRQQNGWTQSELAKKIGIKQATISNFENNPNNTSLTTFFKILQSLELSMTLCDAKNASPEA
TEQQDLEW

Nucleotide

• Length: 267 bp

>AT141428 NZ_CP059960:1069825-1070235 [Pectobacterium brasiliense]
ATGTTTTATCCGATTGCAATTGAAGCAGGCGACGATACCCACGCATACGGTGTAACAGTGCCAGATCTACCGGGCTGTTT
TTCTGCAGGCGATACGCTAGACGATGCCATCGCGAATGCTAAAGAGGCGATTACCGGACATATCGAACTGCTGATCGAGA
TGGGTCAGGATATTCCCACCGTGTCTTCAGTAGGCCAATTGGCAAAAAGCGCAGAATACGCTGGGTACACGTGGGCGGTT
GTTGATATCGATGTGACGCGTTTAATGGGCGGTTCAGAAAAAATTAACGTCACGCTGCCTAAATCACTGATTGACCGAAT
TGATCGTTGCGTGGCGAGTAATCCTGAATTTAAAAGCCGCTCAGGTTTTCTCGCGCAAGCGGCATTAGAGCGAATCTCGT
CTTCCCGATGA

Structures

Toxin

• AlphaFold DB: A0A0E2L5M8