10195

Detailed information of TA system    

experimentalExperimentally validated    

Overview

TA module


Type II Classification (family/domain) hipBA/-
Location 1518116..1519704 Replicon chromosome
Accession NZ_CP010816
Organism Escherichia coli strain BL21 (TaKaRa)

Toxin (Protein)


Gene name hipA Uniprot ID S1ELM3
Locus tag SR36_RS07605 Protein ID WP_001125439.1
Coordinates 1518116..1519438 (-) Length 441 a.a.

Antitoxin (Protein)


Gene name hipB Uniprot ID -
Locus tag SR36_RS07610 Protein ID WP_001301023.1
Coordinates 1519438..1519704 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
SR36_RS25370 1513499..1516149 - 2651 Protein_1447 fimbrial biogenesis outer membrane usher protein -
SR36_RS07595 (SR36_07595) 1516191..1516901 - 711 Protein_1448 fimbria/pilus periplasmic chaperone -
SR36_RS07600 (SR36_07600) 1517262..1517744 - 483 WP_000931227.1 fimbrial protein -
SR36_RS07605 (SR36_07605) 1518116..1519438 - 1323 WP_001125439.1 type II toxin-antitoxin system serine/threonine protein kinase toxin HipA Toxin
SR36_RS07610 (SR36_07610) 1519438..1519704 - 267 WP_001301023.1 type II toxin-antitoxin system antitoxin HipB Antitoxin
SR36_RS07615 (SR36_07615) 1519927..1521507 - 1581 Protein_1452 autotransporter outer membrane beta-barrel domain-containing protein -
SR36_RS07620 (SR36_07620) 1521503..1522279 + 777 Protein_1453 histidine kinase -
SR36_RS07625 (SR36_07625) 1522291..1523313 + 1023 WP_000172488.1 autoinducer 2 ABC transporter substrate-binding protein LsrB -
SR36_RS07630 (SR36_07630) 1523340..1524215 + 876 WP_000774188.1 3-hydroxy-5-phosphonooxypentane-2,4-dione thiolase -
SR36_RS07635 (SR36_07635) 1524239..1524529 + 291 WP_000558527.1 (4S)-4-hydroxy-5-phosphonooxypentane-2,3-dione isomerase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Cro/C1-type helix-turn-helix domain

(21-76)

Antitoxin

Cro/C1-type helix-turn-helix domain

(14-69)


Sequences

Toxin        


Download         Length: 441 a.a.        Molecular weight: 10041.86 Da        Isoelectric Point: 8.5336

>T10195 WP_001125439.1 NZ_CP010816:c1519438-1518116 [Escherichia coli]
MPKLVTWMNNQRVGELTKLANGAHTFKYAPEWLASRYARPLSLSLPLQRGNITSDAVFNFFDNLLPDSPIVRDRIVKRYH
AKSRQPFDLLSEIGRDSVGAVTLIPEDETVTHPIMAWEKLTEARLEEVLTAYKADIPLGMIREENDFRISVAGAQEKTAL
LRIGNDWCIPKGITPTTHIIKLPIGEIRQPNATLDLSQSVDNEYYCLLLAKELGLNVPDAEIIKAGNVRALAVERFDRRW
NAERTVLLRLPQEDMCQTFGLPSSVKYESDGGPGIARIMAFLMGSSEALKDRYDFMKFQVFQWLIGATDGHAKNFSVFIQ
AGGSYRLTPFYDIISAFPVLGGTGIHISDLKLAMGLNASKGKKTAIDKIYPRHFLATAKVLRFPEVQMHEILSDFARMIP
AALDNVKTSLPTDFPENVVTAVESNVLRLHGRLSREYGSK

Download         Length: 1323 bp

>T10195 NZ_CP010816:c1519438-1518116 [Escherichia coli]
ATGCCTAAACTTGTCACTTGGATGAACAACCAGCGGGTAGGCGAGTTAACGAAGTTAGCCAACGGCGCGCACACCTTTAA
GTATGCACCGGAGTGGTTAGCAAGCCGTTATGCCAGACCGTTGTCACTTTCGCTGCCATTGCAGAGGGGGAATATCACCT
CTGATGCCGTATTTAACTTCTTCGATAACCTGTTACCCGATAGCCCGATTGTACGTGACCGGATCGTTAAACGTTATCAT
GCCAAATCCAGACAACCGTTTGATTTATTGTCAGAAATAGGGCGAGACAGCGTTGGTGCCGTGACGTTAATACCCGAAGA
CGAAACCGTAACGCATCCGATAATGGCATGGGAAAAGCTTACTGAAGCCAGACTTGAAGAAGTATTAACGGCTTATAAAG
CAGATATCCCGCTAGGCATGATTAGAGAAGAAAATGACTTTCGCATCTCGGTTGCTGGCGCACAGGAGAAGACAGCACTG
CTCAGAATAGGCAATGACTGGTGCATTCCGAAAGGAATAACGCCGACGACGCACATCATTAAATTACCGATTGGCGAAAT
CAGGCAGCCCAATGCGACGCTCGATCTCAGCCAAAGCGTTGATAATGAGTATTACTGTCTGCTGCTGGCGAAAGAACTTG
GGTTGAATGTTCCGGACGCAGAAATCATTAAAGCGGGAAATGTGCGCGCGTTAGCGGTCGAACGTTTTGACAGGCGTTGG
AATGCTGAGCGAACGGTTTTACTTCGCTTGCCACAGGAGGATATGTGTCAGACATTCGGTTTACCTTCATCGGTGAAATA
TGAATCAGATGGAGGCCCAGGCATCGCGCGGATCATGGCTTTTTTGATGGGGTCCAGCGAGGCGCTGAAAGATCGCTATG
ATTTTATGAAATTCCAGGTCTTCCAGTGGTTGATTGGCGCAACGGACGGTCATGCAAAAAACTTCTCCGTATTTATTCAG
GCTGGCGGCAGTTATCGACTCACGCCATTTTACGACATCATTTCAGCATTTCCGGTCCTTGGCGGTACGGGAATACACAT
CAGCGATCTCAAACTGGCAATGGGGCTTAACGCATCCAAAGGCAAAAAAACGGCAATCGATAAAATTTATCCGCGACATT
TTTTGGCGACAGCAAAGGTGCTGAGATTCCCGGAAGTGCAGATGCATGAAATCCTGAGTGACTTTGCCAGAATGATTCCA
GCAGCACTGGATAACGTGAAGACTTCATTACCGACAGATTTTCCGGAGAACGTGGTGACGGCAGTTGAAAGCAATGTGTT
GAGGTTGCATGGACGGTTAAGCCGAGAATACGGTAGTAAGTGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10016.38 Da        Isoelectric Point: 6.2267

>AT10195 WP_001301023.1 NZ_CP010816:c1519704-1519438 [Escherichia coli]
MMSFQKIYSPTQLANAMKLVRQQNGWTQSELAKKIGIKQATISNFENNPDNTTLTTFFKILQSLELSMTLCDAKNASPES
TEQQNLEW

Download         Length: 267 bp

>AT10195 NZ_CP010816:c1519704-1519438 [Escherichia coli]
ATGATGAGCTTTCAGAAGATCTATAGCCCAACGCAATTGGCGAATGCAATGAAACTGGTTCGCCAGCAAAATGGCTGGAC
GCAGAGCGAGCTGGCGAAAAAAATTGGTATTAAGCAGGCGACGATTTCCAATTTCGAAAACAACCCTGACAATACCACGC
TCACGACATTTTTTAAGATTTTACAGTCGCTTGAACTCTCAATGACGCTATGCGACGCGAAAAATGCCTCGCCAGAATCA
ACAGAACAGCAAAATCTGGAGTGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value
AT1040 Escherichia coli str. K-12 substr. MG1655

100

100

1
AT10098 Escherichia coli Nissle 1917

95.455

100

0.955
AT10106 Xenorhabdus nematophila ATCC 19061

40.964

100

0.41

Multiple sequence alignment    




Structures

Toxin

Source ID Structure
PDB 5K98
PDB 3TPV
PDB 3DNV
PDB 3TPD
PDB 3HZI
PDB 3FBR
PDB 4YG7
PDB 3TPE
PDB 2WIU
PDB 3DNT
PDB 3TPB
PDB 3TPT
PDB 3DNU

Antitoxin

Source ID Structure

References

(1) Yuki Maeda et al. (2017) Characterization of YjjJ toxin of Escherichia coli. FEMS Microbiology Letters 364(11):10.1093/femsle/fnx086. [PubMed:28430938] experimental


experimental experimental literature