Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hipBA/HipA-HipB
Location 1550461..1552049 Replicon chromosome
Accession NZ_CP053600
Organism Escherichia coli strain Nico21(DE3)

Toxin (Protein)


Gene name hipA Uniprot ID S1ELM3
Locus tag HO398_RS07625 Protein ID WP_001125439.1
Coordinates 1550461..1551783 (-) Length 441 a.a.

Antitoxin (Protein)


Gene name hipB Uniprot ID -
Locus tag HO398_RS07630 Protein ID WP_001301023.1
Coordinates 1551783..1552049 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HO398_RS22055 1545844..1548494 - 2651 Protein_1489 fimbrial biogenesis outer membrane usher protein -
HO398_RS07615 1548536..1549246 - 711 Protein_1490 fimbria/pilus periplasmic chaperone -
HO398_RS07620 1549607..1550089 - 483 WP_000931227.1 fimbrial protein -
HO398_RS07625 1550461..1551783 - 1323 WP_001125439.1 type II toxin-antitoxin system serine/threonine protein kinase toxin HipA Toxin
HO398_RS07630 1551783..1552049 - 267 WP_001301023.1 type II toxin-antitoxin system antitoxin HipB Antitoxin
HO398_RS07635 1552272..1553852 - 1581 Protein_1494 autotransporter outer membrane beta-barrel domain-containing protein -
HO398_RS07640 1553848..1554624 + 777 Protein_1495 histidine kinase -
HO398_RS07645 1554636..1555658 + 1023 WP_000172488.1 autoinducer 2 ABC transporter substrate-binding protein LsrB -
HO398_RS07650 1555685..1556560 + 876 WP_000774188.1 3-hydroxy-5-phosphonooxypentane-2,4-dione thiolase -
HO398_RS07655 1556584..1556874 + 291 WP_000558527.1 (4S)-4-hydroxy-5-phosphonooxypentane-2,3-dione isomerase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Genomic island - fimC 1537833..1552049 14216


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HipA-like, C-terminal

(149-401)

Antitoxin

Cro/C1-type helix-turn-helix domain

(14-69)


Sequences

Toxin        


Download         Length: 441 a.a.        Molecular weight: 49275.82 Da        Isoelectric Point: 8.2482

>T156982 WP_001125439.1 NZ_CP053600:c1551783-1550461 [Escherichia coli]
MPKLVTWMNNQRVGELTKLANGAHTFKYAPEWLASRYARPLSLSLPLQRGNITSDAVFNFFDNLLPDSPIVRDRIVKRYH
AKSRQPFDLLSEIGRDSVGAVTLIPEDETVTHPIMAWEKLTEARLEEVLTAYKADIPLGMIREENDFRISVAGAQEKTAL
LRIGNDWCIPKGITPTTHIIKLPIGEIRQPNATLDLSQSVDNEYYCLLLAKELGLNVPDAEIIKAGNVRALAVERFDRRW
NAERTVLLRLPQEDMCQTFGLPSSVKYESDGGPGIARIMAFLMGSSEALKDRYDFMKFQVFQWLIGATDGHAKNFSVFIQ
AGGSYRLTPFYDIISAFPVLGGTGIHISDLKLAMGLNASKGKKTAIDKIYPRHFLATAKVLRFPEVQMHEILSDFARMIP
AALDNVKTSLPTDFPENVVTAVESNVLRLHGRLSREYGSK

Download         Length: 1323 bp

>T156982 NZ_CP069074:c1954207-1953818 [Mycobacterium tuberculosis]
GTGATCGTGTTGGACGCCTCGGCGGCCGTCGAGCTCATGCTCACGACGCCGGCGGGTGCGGCGGTCGCCCGGCGCTTGCG
GGGCGAAACCGTGCACGCCCCTGCACATTTCGATGTCGAGGTGATCGGGGCGATACGTCAAGCGGTCGTGCGTCAGCTGA
TCAGCGATCATGAAGGACTCGTCGTGGTTGTCAACTTCCTTAGCCTGCCCGTTCGGCGTTGGCCGTTAAAGCCATTCACC
CAGCGTGCCTACCAACTTCGGTCCACTCATACCGTGGCCGATGGAGCCTACGTCGCCCTCGCCGAGGGCCTCGGCGTACC
GCTTATCACTTGCGACGGGCGACTTGCCCAATCCCATGGCCATAATGCGGAGATCGAGCTTGTCGCCTGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10016.38 Da        Isoelectric Point: 6.2267

>AT156982 WP_001301023.1 NZ_CP053600:c1552049-1551783 [Escherichia coli]
MMSFQKIYSPTQLANAMKLVRQQNGWTQSELAKKIGIKQATISNFENNPDNTTLTTFFKILQSLELSMTLCDAKNASPES
TEQQNLEW

Download         Length: 267 bp

>AT156982 NZ_CP069074:c1954431-1954204 [Mycobacterium tuberculosis]
ATGTCCGCCATGGTTCAGATCCGCAACGTTCCCGACGAGCTTCTCCACGAGCTGAAGGCCCGCGCAGCGGCGCAACGGAT
GAGCCTGAGCGATTTCCTCCTAGCACGGCTTGCCGAGATCGCGGAAGAACCGGCCCTCGACGACGTTCTCGACCGTCTAG
CGGCACTGCCGCGCCGGGATCTCGGCGCGAGCGCAGCCGAGCTTGTTGACGAGGCTCGATCTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 5K98
PDB 3TPV
PDB 3DNV
PDB 3TPD
PDB 3HZI
PDB 3FBR
PDB 4YG7
PDB 3TPE
PDB 2WIU
PDB 3DNT
PDB 3TPB
PDB 3TPT
PDB 3DNU

Antitoxin

Source ID Structure

References