Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/UPF0150(antitoxin)
Location 2428239..2428877 Replicon chromosome
Accession NZ_CP020543
Organism Escherichia coli C

Toxin (Protein)


Gene name hicA Uniprot ID S1F9G9
Locus tag B6N50_RS12420 Protein ID WP_000813794.1
Coordinates 2428701..2428877 (-) Length 59 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID -
Locus tag B6N50_RS12415 Protein ID WP_001270286.1
Coordinates 2428239..2428655 (-) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
B6N50_RS12395 (B6N50_12395) 2423391..2424332 - 942 WP_001251304.1 ABC transporter permease -
B6N50_RS12400 (B6N50_12400) 2424333..2425346 - 1014 WP_000220396.1 ABC transporter ATP-binding protein -
B6N50_RS12405 (B6N50_12405) 2425364..2426509 - 1146 WP_000047424.1 ABC transporter substrate-binding protein -
B6N50_RS12410 (B6N50_12410) 2426754..2428160 - 1407 WP_000760626.1 PLP-dependent aminotransferase family protein -
B6N50_RS12415 (B6N50_12415) 2428239..2428655 - 417 WP_001270286.1 type II toxin-antitoxin system antitoxin HicB Antitoxin
B6N50_RS12420 (B6N50_12420) 2428701..2428877 - 177 WP_000813794.1 type II toxin-antitoxin system mRNA interferase toxin HicA Toxin
B6N50_RS12425 (B6N50_12425) 2429099..2429329 + 231 WP_000494244.1 YncJ family protein -
B6N50_RS12430 (B6N50_12430) 2429421..2431382 - 1962 WP_001303492.1 23S rRNA 5-hydroxycytidine C2501 synthase -
B6N50_RS12435 (B6N50_12435) 2431455..2431991 - 537 WP_000429155.1 DNA-binding transcriptional regulator SutR -
B6N50_RS12440 (B6N50_12440) 2432044..2433258 + 1215 WP_001326689.1 BenE family transporter YdcO -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
flank IS/Tn - - 2433298..2434446 1148


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(5-57)

Antitoxin

Cro/C1-type helix-turn-helix domain

(91-134)


Sequences

Toxin        


Download         Length: 59 a.a.        Molecular weight: 6749.83 Da        Isoelectric Point: 11.2298

>T75073 WP_000813794.1 NZ_CP020543:c2428877-2428701 [Escherichia coli C]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Download         Length: 177 bp

>T75073 NZ_CP020543:c2428877-2428701 [Escherichia coli C]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 15246.63 Da        Isoelectric Point: 4.7386

>AT75073 WP_001270286.1 NZ_CP020543:c2428655-2428239 [Escherichia coli C]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Download         Length: 417 bp

>AT75073 NZ_CP020543:c2428655-2428239 [Escherichia coli C]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
CCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCTTTACCTTCGCCATTAAATAGTCACGATCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAGGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAGGAGAT
TACTCGCCTATTTAACTTGCATCATGCGACAAAAATCGACGCCGTCCAGCTCGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6HPB

Antitoxin

Source ID Structure

References