Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/UPF0150(antitoxin)
Location 1554641..1555279 Replicon chromosome
Accession NZ_CP029239
Organism Escherichia coli strain BH212

Toxin (Protein)


Gene name hicA Uniprot ID S1F9G9
Locus tag DF195_RS07785 Protein ID WP_000813794.1
Coordinates 1554641..1554817 (+) Length 59 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID -
Locus tag DF195_RS07790 Protein ID WP_001270286.1
Coordinates 1554863..1555279 (+) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
DF195_RS07765 1550260..1551474 - 1215 WP_001326689.1 BenE family transporter YdcO -
DF195_RS07770 1551527..1552063 + 537 WP_000429155.1 DNA-binding transcriptional regulator SutR -
DF195_RS07775 1552136..1554097 + 1962 WP_001303492.1 23S rRNA 5-hydroxycytidine C2501 synthase -
DF195_RS07780 1554189..1554419 - 231 WP_000494244.1 YncJ family protein -
DF195_RS07785 1554641..1554817 + 177 WP_000813794.1 type II toxin-antitoxin system mRNA interferase toxin HicA Toxin
DF195_RS07790 1554863..1555279 + 417 WP_001270286.1 type II toxin-antitoxin system antitoxin HicB Antitoxin
DF195_RS07795 1555358..1556764 + 1407 WP_000760626.1 PLP-dependent aminotransferase family protein -
DF195_RS07800 1557009..1558154 + 1146 WP_000047424.1 ABC transporter substrate-binding protein -
DF195_RS07805 1558172..1559185 + 1014 WP_000220396.1 ABC transporter ATP-binding protein -
DF195_RS07810 1559186..1560127 + 942 WP_001251304.1 ABC transporter permease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
flank IS/Tn - - 1549072..1550220 1148


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(5-57)

Antitoxin

Cro/C1-type helix-turn-helix domain

(91-134)


Sequences

Toxin        


Download         Length: 59 a.a.        Molecular weight: 6749.83 Da        Isoelectric Point: 11.2298

>T105285 WP_000813794.1 NZ_CP029239:1554641-1554817 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Download         Length: 177 bp

>T105285 NZ_CP029239:1554641-1554817 [Escherichia coli]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 15246.63 Da        Isoelectric Point: 4.7386

>AT105285 WP_001270286.1 NZ_CP029239:1554863-1555279 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Download         Length: 417 bp

>AT105285 NZ_CP029239:1554863-1555279 [Escherichia coli]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
CCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCTTTACCTTCGCCATTAAATAGTCACGATCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAGGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAGGAGAT
TACTCGCCTATTTAACTTGCATCATGCGACAAAAATCGACGCCGTCCAGCTCGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6HPB

Antitoxin

Source ID Structure

References