Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/UPF0150(antitoxin)
Location 2541569..2542207 Replicon chromosome
Accession NZ_CP086500
Organism Escherichia coli strain Ec-050-T5-MAC

Toxin (Protein)


Gene name hicA Uniprot ID A0A0A6TXU8
Locus tag LNN49_RS12170 Protein ID WP_001447010.1
Coordinates 2542031..2542207 (-) Length 59 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID -
Locus tag LNN49_RS12165 Protein ID WP_001270286.1
Coordinates 2541569..2541985 (-) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LNN49_RS12145 (2536721) 2536721..2537662 - 942 WP_001251304.1 ABC transporter permease -
LNN49_RS12150 (2537663) 2537663..2538676 - 1014 WP_000220396.1 ABC transporter ATP-binding protein -
LNN49_RS12155 (2538694) 2538694..2539839 - 1146 WP_000047424.1 ABC transporter substrate-binding protein -
LNN49_RS12160 (2540084) 2540084..2541490 - 1407 WP_000760626.1 PLP-dependent aminotransferase family protein -
LNN49_RS12165 (2541569) 2541569..2541985 - 417 WP_001270286.1 type II toxin-antitoxin system antitoxin HicB Antitoxin
LNN49_RS12170 (2542031) 2542031..2542207 - 177 WP_001447010.1 type II toxin-antitoxin system mRNA interferase toxin HicA Toxin
LNN49_RS12175 (2542429) 2542429..2542659 + 231 WP_000494244.1 YncJ family protein -
LNN49_RS12180 (2542751) 2542751..2544712 - 1962 WP_001303492.1 23S rRNA 5-hydroxycytidine C2501 synthase -
LNN49_RS12185 (2544785) 2544785..2545321 - 537 WP_000429155.1 DNA-binding transcriptional regulator SutR -
LNN49_RS12190 (2545413) 2545413..2546588 + 1176 WP_001236265.1 BenE family transporter YdcO -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
flank IS/Tn - - 2546628..2547776 1148


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin


No domain identified.


Antitoxin

Cro/C1-type helix-turn-helix domain

(91-134)


Sequences

Toxin        


Download         Length: 59 a.a.        Molecular weight: 6779.86 Da        Isoelectric Point: 11.2298

>T223595 WP_001447010.1 NZ_CP086500:c2542207-2542031 [Escherichia coli]
VKQSEFRRWLESQGVDVANSSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Download         Length: 177 bp

>T223595 NZ_CP118990:c173497-173141 [Aeromonas allosaccharophila]
ATGACAAACAAAGAAAAACCGCTCGAATGGATCGCGAGCAGCCACAAGGATTTGATGGCGTTGCCGTCCGACGTGCGTCG
CCGTTTCGGTTACGCGCTCTCGTTGGCGCAGATAGGCGATCAGGATGACGCAGCAAAGGAGCTCAAGGGGTTCGGTGGTG
CCGGCGTGCTGAAGGTCGTCGAAGACGATGCCGGCGGCACCTATCGAGCGGTATACACGGTCAAGTTTGCGGAAGCGGTG
TTCGTCCTGCACTGCTTCCAGAAGAAGAGCAAGAGCGGAATCGCCACGCCAAAGGCCGACATGGACATCATCCGCGCTCG
GCTGAAGGTGGCCGAGGTATTGGCACAGGAGCTATGA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 15246.63 Da        Isoelectric Point: 4.7386

>AT223595 WP_001270286.1 NZ_CP086500:c2541985-2541569 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Download         Length: 417 bp

>AT223595 NZ_CP118990:c173139-172804 [Aeromonas allosaccharophila]
ATGCAAAAACGAATCATTGAAGGCGTCGAGGTTCAGCGCAGCTCGGGCAACGTCTTTGCCGACCTTGGACTGCCTGACGC
TGAAAAGCTCAAGATCAAGACCGGCCTGGTGGTCGAGATCAGGAGGGCCATGCGCGCCCTTGGGCTGACTCAACAAGCGG
CGGCCAAACGCATGGGCATCCCGCAACCGAAGGTGTCGGGCATGATGCGCGGCGACTTCACCAATCTATCCGAACGCAAG
CTGATGGATTGTCTGAATCGCCTCGGCTACGACATCGAAATCAAGGTACGGCCAGCAGCCGAGCCGATCGGGCATCTAAC
GCTCGCAACCGCTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0A6TXU8

Antitoxin

Source ID Structure

References