Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/UPF0150(antitoxin)
Location 2764777..2765415 Replicon chromosome
Accession NZ_CP074007
Organism Escherichia coli strain PD4

Toxin (Protein)


Gene name hicA Uniprot ID S1F9G9
Locus tag KFU74_RS13270 Protein ID WP_000813794.1
Coordinates 2765239..2765415 (-) Length 59 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID -
Locus tag KFU74_RS13265 Protein ID WP_001270286.1
Coordinates 2764777..2765193 (-) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KFU74_RS13245 (2759929) 2759929..2760870 - 942 WP_001251319.1 ABC transporter permease -
KFU74_RS13250 (2760871) 2760871..2761884 - 1014 WP_104769175.1 ABC transporter ATP-binding protein -
KFU74_RS13255 (2761902) 2761902..2763047 - 1146 WP_000047429.1 ABC transporter substrate-binding protein -
KFU74_RS13260 (2763289) 2763289..2764698 - 1410 WP_000760625.1 PLP-dependent aminotransferase family protein -
KFU74_RS13265 (2764777) 2764777..2765193 - 417 WP_001270286.1 type II toxin-antitoxin system antitoxin HicB Antitoxin
KFU74_RS13270 (2765239) 2765239..2765415 - 177 WP_000813794.1 type II toxin-antitoxin system mRNA interferase toxin HicA Toxin
KFU74_RS13275 (2765637) 2765637..2765867 + 231 WP_000494244.1 YncJ family protein -
KFU74_RS13280 (2765959) 2765959..2767920 - 1962 WP_001303492.1 23S rRNA 5-hydroxycytidine C2501 synthase -
KFU74_RS13285 (2767993) 2767993..2768529 - 537 WP_000429155.1 DNA-binding transcriptional regulator SutR -
KFU74_RS13290 (2768621) 2768621..2769796 + 1176 WP_001236265.1 BenE family transporter YdcO -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
flank IS/Tn - - 2769836..2770984 1148


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(5-57)

Antitoxin

Cro/C1-type helix-turn-helix domain

(91-134)


Sequences

Toxin        


Download         Length: 59 a.a.        Molecular weight: 6749.83 Da        Isoelectric Point: 11.2298

>T201065 WP_000813794.1 NZ_CP074007:c2765415-2765239 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Download         Length: 177 bp

>T201065 NZ_CP098214:3660090-3660308 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGTCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCTGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 15246.63 Da        Isoelectric Point: 4.7386

>AT201065 WP_001270286.1 NZ_CP074007:c2765193-2764777 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Download         Length: 417 bp

>AT201065 NZ_CP098214:3659690-3660064 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6HPB

Antitoxin

Source ID Structure

References