Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/UPF0150(antitoxin)
Location 2541567..2542205 Replicon chromosome
Accession NZ_CP086480
Organism Escherichia coli strain Ec-050-T20-MAC

Toxin (Protein)


Gene name hicA Uniprot ID A0A0A6TXU8
Locus tag LNN46_RS12180 Protein ID WP_001447010.1
Coordinates 2542029..2542205 (-) Length 59 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID -
Locus tag LNN46_RS12175 Protein ID WP_001270286.1
Coordinates 2541567..2541983 (-) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LNN46_RS12155 (2536719) 2536719..2537660 - 942 WP_001251304.1 ABC transporter permease -
LNN46_RS12160 (2537661) 2537661..2538674 - 1014 WP_000220396.1 ABC transporter ATP-binding protein -
LNN46_RS12165 (2538692) 2538692..2539837 - 1146 WP_000047424.1 ABC transporter substrate-binding protein -
LNN46_RS12170 (2540082) 2540082..2541488 - 1407 WP_000760626.1 PLP-dependent aminotransferase family protein -
LNN46_RS12175 (2541567) 2541567..2541983 - 417 WP_001270286.1 type II toxin-antitoxin system antitoxin HicB Antitoxin
LNN46_RS12180 (2542029) 2542029..2542205 - 177 WP_001447010.1 type II toxin-antitoxin system mRNA interferase toxin HicA Toxin
LNN46_RS12185 (2542427) 2542427..2542657 + 231 WP_000494244.1 YncJ family protein -
LNN46_RS12190 (2542749) 2542749..2544710 - 1962 WP_001303492.1 23S rRNA 5-hydroxycytidine C2501 synthase -
LNN46_RS12195 (2544783) 2544783..2545319 - 537 WP_000429155.1 DNA-binding transcriptional regulator SutR -
LNN46_RS12200 (2545372) 2545372..2546586 + 1215 WP_001326689.1 BenE family transporter YdcO -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
flank IS/Tn - - 2546626..2547774 1148


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin


No domain identified.


Antitoxin

Cro/C1-type helix-turn-helix domain

(91-134)


Sequences

Toxin        


Download         Length: 59 a.a.        Molecular weight: 6779.86 Da        Isoelectric Point: 11.2298

>T223512 WP_001447010.1 NZ_CP086480:c2542205-2542029 [Escherichia coli]
VKQSEFRRWLESQGVDVANSSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Download         Length: 177 bp

>T223512 NZ_CP118932:c1027464-1027261 [Serratia marcescens]
ATGACTAAAACCGACTATCTGATGCGTTTACGTAAATGCACCACGATTGATACTCTCGAACGTGTTATTGAGAAAAATAA
GTACGAGCTTTCCGATGATGAACTGGAGCTGTTTTACTCAGCGGCAGATCATCGTCTGGCAGAGCTCACCATGAACAAAC
TGTATGACAAAATCCCCACCTCCGTTTGGAAATATGTCAGATAA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 15246.63 Da        Isoelectric Point: 4.7386

>AT223512 WP_001270286.1 NZ_CP086480:c2541983-2541567 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Download         Length: 417 bp

>AT223512 NZ_CP118932:c1027882-1027514 [Serratia marcescens]
ATGGATGAGTATTCGCCTAAGCGACACGACATTGCTCAGCTCAAATTCTTGTGCGAAAATTTGTATGATGAAGGTATCGC
TACCCTGGGAGACAGTCATCATGGGTGGGTCAACGATCCGACGTCCTCCGTCAACCTGCAGCTTAATGAGTTGATCGAAC
ATATCGCTTCGTTTGTGATGAGTTATAAAATTAAATACATGGACGAATCTGACCTGTCGGAGTTAGTCGAAGAATATCTC
GACGATACCTATACGCTGTTCAGCAGCTATGGTATCAATGATTCCGACCTGCGCCGCTGGCAAAAAACCAAGGCGAGATT
ATTCAGAATGTTCTCAGGAGAGGACATCTGTACGACAATGAAAACTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0A6TXU8

Antitoxin

Source ID Structure

References