Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/UPF0150(antitoxin)
Location 583103..583741 Replicon chromosome
Accession NZ_CP075737
Organism Escherichia coli strain CPF6

Toxin (Protein)


Gene name hicA Uniprot ID S1F9G9
Locus tag KKZ13_RS02880 Protein ID WP_000813794.1
Coordinates 583103..583279 (+) Length 59 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID -
Locus tag KKZ13_RS02885 Protein ID WP_001270286.1
Coordinates 583325..583741 (+) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KKZ13_RS02860 (578722) 578722..579897 - 1176 WP_001236265.1 BenE family transporter YdcO -
KKZ13_RS02865 (579989) 579989..580525 + 537 WP_000429155.1 DNA-binding transcriptional regulator SutR -
KKZ13_RS02870 (580598) 580598..582559 + 1962 WP_001303492.1 23S rRNA 5-hydroxycytidine C2501 synthase -
KKZ13_RS02875 (582651) 582651..582881 - 231 WP_000494244.1 YncJ family protein -
KKZ13_RS02880 (583103) 583103..583279 + 177 WP_000813794.1 type II toxin-antitoxin system mRNA interferase toxin HicA Toxin
KKZ13_RS02885 (583325) 583325..583741 + 417 WP_001270286.1 type II toxin-antitoxin system antitoxin HicB Antitoxin
KKZ13_RS02890 (583820) 583820..585226 + 1407 WP_000760626.1 PLP-dependent aminotransferase family protein -
KKZ13_RS02895 (585471) 585471..586616 + 1146 WP_000047424.1 ABC transporter substrate-binding protein -
KKZ13_RS02900 (586634) 586634..587647 + 1014 WP_000220396.1 ABC transporter ATP-binding protein -
KKZ13_RS02905 (587648) 587648..588589 + 942 WP_001251304.1 ABC transporter permease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
flank IS/Tn - - 577534..578682 1148


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(5-57)

Antitoxin

Cro/C1-type helix-turn-helix domain

(91-134)


Sequences

Toxin        


Download         Length: 59 a.a.        Molecular weight: 6749.83 Da        Isoelectric Point: 11.2298

>T204370 WP_000813794.1 NZ_CP075737:583103-583279 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Download         Length: 177 bp

>T204370 NZ_CP100710:c1041799-1041272 [Salmonella enterica subsp. enterica serovar Blockley]
ATGATGTTTACAGACTGGCATGAGGCCGCGATAGGGAAAACCCACAATCGAATGAATTTTGATTGTGGGGATGCCGATCT
GAACCAATTCCTGCAACGCCATGCACGACAAAATCATGAGAAAGGGACAACGAAAACCTATGTTGCGCTTGATAATTCGG
ATGTTACACGTATCCACGGCTTTTACTCAGTCAGTCCTGCATCACTGATATATGCACAGGTTCCCGGCGCAATCAGCAAA
GGATTATGGAGATACGATGTGCCAGTGTTTCGTCTGGGTCGTTTAGCCGTAGACAAATCTATGCAGGGGCAAGGGCTGGG
AGCACAACTTTTGTTATCTGCCGGAAAGCGTTGCATACAGGCGGCTTTGCAGGTCGGTGGCGTAGCCCTACTTATTGATG
CCAAAAATAAACAGGCCTGCGACTGGTACAAAGGATTTGGCGCAGTACCATTAAACGATCAACCCCTTTCCTTGTTGCTG
TCGTTTAAAACGCTTTATGCTGCTTTATCTGCATCTGGTAGGTTATGA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 15246.63 Da        Isoelectric Point: 4.7386

>AT204370 WP_001270286.1 NZ_CP075737:583325-583741 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Download         Length: 417 bp

>AT204370 NZ_CP100710:c1042086-1041796 [Salmonella enterica subsp. enterica serovar Blockley]
ATGAAAACCATGCCTCAGATAGCTATAGAATCTAACGAAAGGCTGTCGTTGCGTGTATCGACGGATGCCAAAAAGTTGAT
AGTCCGCGCTGCGGCAATCCAGCAAACCAACCTGACGGATTTTGTGGTCAGTAACGTACTACCTGTCGCACAGAAGATTG
TTGATGCCGCCGAACGGGTATATCTGACGGAGCGTGATACTCAAATGATCATGGAAATTTTAGATAACCCGCCAGCGCCG
AATGAAAAGCTTCTTGCTGCTGCTTTCGCCCTGCCGGACATGAAGAAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6HPB

Antitoxin

Source ID Structure

References