Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/UPF0150(antitoxin)
Location 2404515..2405153 Replicon chromosome
Accession NZ_AP027445
Organism Escherichia coli strain EC291 isolate EC291

Toxin (Protein)


Gene name hicA Uniprot ID S1F9G9
Locus tag QUE55_RS13525 Protein ID WP_000813794.1
Coordinates 2404977..2405153 (-) Length 59 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID -
Locus tag QUE55_RS13520 Protein ID WP_001270286.1
Coordinates 2404515..2404931 (-) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
QUE55_RS13500 (2399667) 2399667..2400608 - 942 WP_149506168.1 ABC transporter permease -
QUE55_RS13505 (2400609) 2400609..2401622 - 1014 WP_149506167.1 ABC transporter ATP-binding protein -
QUE55_RS13510 (2401640) 2401640..2402785 - 1146 WP_000047429.1 ABC transporter substrate-binding protein -
QUE55_RS13515 (2403027) 2403027..2404436 - 1410 WP_000760625.1 PLP-dependent aminotransferase family protein -
QUE55_RS13520 (2404515) 2404515..2404931 - 417 WP_001270286.1 type II toxin-antitoxin system antitoxin HicB Antitoxin
QUE55_RS13525 (2404977) 2404977..2405153 - 177 WP_000813794.1 type II toxin-antitoxin system mRNA interferase toxin HicA Toxin
QUE55_RS13530 (2405375) 2405375..2405605 + 231 WP_000494244.1 YncJ family protein -
QUE55_RS13535 (2405697) 2405697..2407658 - 1962 WP_001303492.1 23S rRNA 5-hydroxycytidine C2501 synthase -
QUE55_RS13540 (2407731) 2407731..2408267 - 537 WP_000429155.1 DNA-binding transcriptional regulator SutR -
QUE55_RS13545 (2408359) 2408359..2409534 + 1176 WP_001236265.1 BenE family transporter YdcO -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
flank IS/Tn - - 2409574..2410722 1148


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(5-57)

Antitoxin

Cro/C1-type helix-turn-helix domain

(91-134)


Sequences

Toxin        


Download         Length: 59 a.a.        Molecular weight: 6749.83 Da        Isoelectric Point: 11.2298

>T44122 WP_000813794.1 NZ_AP027445:c2405153-2404977 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Download         Length: 177 bp

>T44122 NZ_AP027445:c2405153-2404977 [Escherichia coli]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 15246.63 Da        Isoelectric Point: 4.7386

>AT44122 WP_001270286.1 NZ_AP027445:c2404931-2404515 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Download         Length: 417 bp

>AT44122 NZ_AP027445:c2404931-2404515 [Escherichia coli]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
GCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCTTTACCTTCGCCATTAAATAGTCACGATCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAGGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAGGAGAT
TACTCGCCTATTTAACTTGCATCATGCGACAAAAATCGACGCCGTCCAGCTCGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6HPB

Antitoxin

Source ID Structure

References