Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/UPF0150(antitoxin)
Location 1317022..1317660 Replicon chromosome
Accession NZ_CP079772
Organism Escherichia coli strain GS04

Toxin (Protein)


Gene name hicA Uniprot ID S1F9G9
Locus tag KYX57_RS06550 Protein ID WP_000813794.1
Coordinates 1317484..1317660 (-) Length 59 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID -
Locus tag KYX57_RS06545 Protein ID WP_001270286.1
Coordinates 1317022..1317438 (-) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KYX57_RS06525 (1312174) 1312174..1313115 - 942 WP_001251319.1 ABC transporter permease -
KYX57_RS06530 (1313116) 1313116..1314129 - 1014 WP_000220393.1 ABC transporter ATP-binding protein -
KYX57_RS06535 (1314147) 1314147..1315292 - 1146 WP_000047418.1 ABC transporter substrate-binding protein -
KYX57_RS06540 (1315537) 1315537..1316943 - 1407 WP_000760626.1 PLP-dependent aminotransferase family protein -
KYX57_RS06545 (1317022) 1317022..1317438 - 417 WP_001270286.1 type II toxin-antitoxin system antitoxin HicB Antitoxin
KYX57_RS06550 (1317484) 1317484..1317660 - 177 WP_000813794.1 type II toxin-antitoxin system mRNA interferase toxin HicA Toxin
KYX57_RS06555 (1317882) 1317882..1318112 + 231 WP_000494244.1 YncJ family protein -
KYX57_RS06560 (1318204) 1318204..1320165 - 1962 WP_001303492.1 23S rRNA 5-hydroxycytidine C2501 synthase -
KYX57_RS06565 (1320238) 1320238..1320774 - 537 WP_000429155.1 DNA-binding transcriptional regulator SutR -
KYX57_RS06570 (1320866) 1320866..1322041 + 1176 WP_032253412.1 BenE family transporter YdcO -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
flank IS/Tn - - 1322081..1323229 1148


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(5-57)

Antitoxin

Cro/C1-type helix-turn-helix domain

(91-134)


Sequences

Toxin        


Download         Length: 59 a.a.        Molecular weight: 6749.83 Da        Isoelectric Point: 11.2298

>T210026 WP_000813794.1 NZ_CP079772:c1317660-1317484 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Download         Length: 177 bp

>T210026 NZ_CP104371:104746-105000 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAGTCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CATTGAACTTATCAAAGATACCCGCAGAACACCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAGCATAATTTGTCAG
GCTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 15246.63 Da        Isoelectric Point: 4.7386

>AT210026 WP_001270286.1 NZ_CP079772:c1317438-1317022 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Download         Length: 417 bp

>AT210026 NZ_CP104371:104498-104749 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGCCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGCTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6HPB

Antitoxin

Source ID Structure

References