Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/UPF0150(antitoxin)
Location 965037..965675 Replicon chromosome
Accession NZ_CP014642
Organism Escherichia coli strain BLK16

Toxin (Protein)


Gene name hicA Uniprot ID S1F9G9
Locus tag AYR48_RS04990 Protein ID WP_000813794.1
Coordinates 965037..965213 (+) Length 59 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID -
Locus tag AYR48_RS04995 Protein ID WP_001270286.1
Coordinates 965259..965675 (+) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AYR48_RS04970 (960656) 960656..961831 - 1176 WP_001236265.1 BenE family transporter YdcO -
AYR48_RS04975 (961923) 961923..962459 + 537 WP_000429155.1 DNA-binding transcriptional regulator SutR -
AYR48_RS04980 (962532) 962532..964493 + 1962 WP_001301045.1 23S rRNA 5-hydroxycytidine C2501 synthase -
AYR48_RS04985 (964585) 964585..964815 - 231 WP_000494244.1 YncJ family protein -
AYR48_RS04990 (965037) 965037..965213 + 177 WP_000813794.1 type II toxin-antitoxin system mRNA interferase toxin HicA Toxin
AYR48_RS04995 (965259) 965259..965675 + 417 WP_001270286.1 type II toxin-antitoxin system antitoxin HicB Antitoxin
AYR48_RS05000 (965754) 965754..967160 + 1407 WP_000760626.1 PLP-dependent aminotransferase family protein -
AYR48_RS05005 (967405) 967405..968550 + 1146 WP_000047424.1 ABC transporter substrate-binding protein -
AYR48_RS05010 (968568) 968568..969581 + 1014 WP_000220396.1 ABC transporter ATP-binding protein -
AYR48_RS05015 (969582) 969582..970523 + 942 WP_001251313.1 ABC transporter permease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
flank IS/Tn - - 959468..960616 1148


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(5-57)

Antitoxin

Cro/C1-type helix-turn-helix domain

(91-134)


Sequences

Toxin        


Download         Length: 59 a.a.        Molecular weight: 6749.83 Da        Isoelectric Point: 11.2298

>T61601 WP_000813794.1 NZ_CP014642:965037-965213 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Download         Length: 177 bp

>T61601 NZ_CP014642:965037-965213 [Escherichia coli]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 15246.63 Da        Isoelectric Point: 4.7386

>AT61601 WP_001270286.1 NZ_CP014642:965259-965675 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Download         Length: 417 bp

>AT61601 NZ_CP014642:965259-965675 [Escherichia coli]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
GCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCTTTACCTTCGCCATTAAATAGTCATGATCACTTTATTGAAGTGCCTTTGAGCGTCGCCTCTAAGGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAGGAGAT
TACTCGCCTATTTAACTTGCATCATGCGACAAAAATCGACGCCGTCCAGCTCGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6HPB

Antitoxin

Source ID Structure

References