Detailed information of TA system TA171869

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 2418696..2419334
• Replicon: chromosome
• Accession: NZ_CP060979
• Organism: Escherichia coli strain EC3

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: A0A0A6TXU8
• Locus tag: H9191_RS11985
• Protein ID: WP_001447010.1
• Coordinates: 2419158..2419334 (-)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: H9191_RS11980
• Protein ID: WP_001270286.1
• Coordinates: 2418696..2419112 (-)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H9191_RS11960 (2413848)	2413848..2414789	-	942	WP_001251304.1	ABC transporter permease	-
H9191_RS11965 (2414790)	2414790..2415803	-	1014	WP_000220396.1	ABC transporter ATP-binding protein	-
H9191_RS11970 (2415821)	2415821..2416966	-	1146	WP_000047424.1	ABC transporter substrate-binding protein	-
H9191_RS11975 (2417211)	2417211..2418617	-	1407	WP_000760626.1	PLP-dependent aminotransferase family protein	-
H9191_RS11980 (2418696)	2418696..2419112	-	417	WP_001270286.1	type II toxin-antitoxin system antitoxin HicB	Antitoxin
H9191_RS11985 (2419158)	2419158..2419334	-	177	WP_001447010.1	type II toxin-antitoxin system mRNA interferase toxin HicA	Toxin
H9191_RS11990 (2419556)	2419556..2419786	+	231	WP_000494244.1	YncJ family protein	-
H9191_RS11995 (2419878)	2419878..2421839	-	1962	WP_001303492.1	23S rRNA 5-hydroxycytidine C2501 synthase	-
H9191_RS12000 (2421912)	2421912..2422448	-	537	WP_000429155.1	DNA-binding transcriptional regulator SutR	-
H9191_RS12005 (2422540)	2422540..2423715	+	1176	WP_001236265.1	BenE family transporter YdcO	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	Similar MGE(s) Similar MGE(s) is defined as other TA-related MGE(s) with Mash distance < 0.01 to this MGE. Detail Genome accession Organism MGE type Related TA Coordinates Mash distance View NZ_CP020543 Escherichia coli C IS/Tn TA075073 2433298..2434446 0.008	flank	IS/Tn	-	-	2423755..2424903	1148

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (91-134)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6779.86 Da
• Isoelectric Point: 11.2298

>T171869 WP_001447010.1 NZ_CP060979:c2419334-2419158 [Escherichia coli]
VKQSEFRRWLESQGVDVANSSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Nucleotide

• Length: 177 bp

>T171869 NZ_CP078145:c6356924-6356499 [Nocardia iowensis]
GTGAGGAACTATCTGCTCGATACGAGTGCGGTGTCGGAATGGGTGAAGCCTCGGCCGGATCCCGGCCTCACCCAATGGCT
CCACACGACTGACGAGGACCGGCTTCATCTCAGCGTCATCACCCTCGGCGAAATCCGTAAGGGCATCGAGAAGATGCCGG
ATTCGCCCAAGAAGCGGCGGCTGGTGGAGTGGCTGACGGAATCGCTGATCGACCGGTTCGAAGGGCGCCTCCTGTCGGTA
GACGCGACAGTCGCACAAGCATGGGGACGGATGGTTGCGCGAACTGAAGGGAACGGAAATCCGGTCGAGCCGGCGGACGC
GCTGATCGCGGCGACCGCCGTATCGCACGGGCTGGAGGTTGTCACGCGGAATGTCGGACATTTCGGACCGACCGGGGTGG
CGATCGTATGTCCCTGGCACGGCTAG

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15246.63 Da
• Isoelectric Point: 4.7386

>AT171869 WP_001270286.1 NZ_CP060979:c2419112-2418696 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Nucleotide

• Length: 417 bp

>AT171869 NZ_CP078145:c6357217-6356921 [Nocardia iowensis]
GTGGTCGAAACGGGTGATCAGATGGCTCGAAAGCGGTCGGAGCGACCTGACGTCGCATGGCCACTTGCCGACGCGAAGGC
CAGGTTGTCCGAGTTGATCGACATCGTCGAGCGGGAGGGGCCGCAAGTGATCAGCAAGCATGGACGAGAGGTCGCTGTTG
TCGTACCGATCGACGAGTGGCGGCAGAAAACGAGCAGGCAGGGGAGCCTCGCCGAGTTCTTCGCGGCTTCGCCGCTGCGC
GATTCCGGAGTAGAGATCGAACGCATCCATATGGATGTCCGTGACGTCGAGCTGTGA

Structures

Toxin

• AlphaFold DB: A0A0A6TXU8