Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/UPF0150(antitoxin)
Location 2342976..2343614 Replicon chromosome
Accession NZ_AP021895
Organism Escherichia coli strain 2018-11-3CC

Toxin (Protein)


Gene name hicA Uniprot ID S1F9G9
Locus tag HRR26_RS11220 Protein ID WP_000813794.1
Coordinates 2343438..2343614 (-) Length 59 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID -
Locus tag HRR26_RS11215 Protein ID WP_001270286.1
Coordinates 2342976..2343392 (-) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HRR26_RS11195 2338128..2339069 - 942 WP_001251319.1 ABC transporter permease -
HRR26_RS11200 2339070..2340083 - 1014 WP_000220393.1 ABC transporter ATP-binding protein -
HRR26_RS11205 2340101..2341246 - 1146 WP_000047418.1 ABC transporter substrate-binding protein -
HRR26_RS11210 2341491..2342897 - 1407 WP_000760626.1 PLP-dependent aminotransferase family protein -
HRR26_RS11215 2342976..2343392 - 417 WP_001270286.1 type II toxin-antitoxin system antitoxin HicB Antitoxin
HRR26_RS11220 2343438..2343614 - 177 WP_000813794.1 type II toxin-antitoxin system mRNA interferase toxin HicA Toxin
HRR26_RS11225 2343836..2344066 + 231 WP_000494244.1 YncJ family protein -
HRR26_RS11230 2344158..2346119 - 1962 WP_001303492.1 23S rRNA 5-hydroxycytidine C2501 synthase -
HRR26_RS11235 2346192..2346728 - 537 WP_000429155.1 DNA-binding transcriptional regulator SutR -
HRR26_RS11240 2346781..2347995 + 1215 WP_072003393.1 BenE family transporter YdcO -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
flank IS/Tn - - 2348035..2349183 1148


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(5-57)

Antitoxin

Cro/C1-type helix-turn-helix domain

(91-134)


Sequences

Toxin        


Download         Length: 59 a.a.        Molecular weight: 6749.83 Da        Isoelectric Point: 11.2298

>T33647 WP_000813794.1 NZ_AP021895:c2343614-2343438 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Download         Length: 177 bp

>T33647 NZ_AP021895:c2343614-2343438 [Escherichia coli]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 15246.63 Da        Isoelectric Point: 4.7386

>AT33647 WP_001270286.1 NZ_AP021895:c2343392-2342976 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Download         Length: 417 bp

>AT33647 NZ_AP021895:c2343392-2342976 [Escherichia coli]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
CCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCTTTACCTTCGCCATTAAATAGTCACGATCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAGGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAGGAGAT
TACTCGCCTATTTAACTTGCATCATGCGACAAAAATCGACGCCGTCCAGCTCGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6HPB

Antitoxin

Source ID Structure

References