Detailed information of TA system TA033647

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 2342976..2343614
• Replicon: chromosome
• Accession: NZ_AP021895
• Organism: Escherichia coli strain 2018-11-3CC

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: S1F9G9
• Locus tag: HRR26_RS11220
• Protein ID: WP_000813794.1
• Coordinates: 2343438..2343614 (-)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: HRR26_RS11215
• Protein ID: WP_001270286.1
• Coordinates: 2342976..2343392 (-)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HRR26_RS11195	2338128..2339069	-	942	WP_001251319.1	ABC transporter permease	-
HRR26_RS11200	2339070..2340083	-	1014	WP_000220393.1	ABC transporter ATP-binding protein	-
HRR26_RS11205	2340101..2341246	-	1146	WP_000047418.1	ABC transporter substrate-binding protein	-
HRR26_RS11210	2341491..2342897	-	1407	WP_000760626.1	PLP-dependent aminotransferase family protein	-
HRR26_RS11215	2342976..2343392	-	417	WP_001270286.1	type II toxin-antitoxin system antitoxin HicB	Antitoxin
HRR26_RS11220	2343438..2343614	-	177	WP_000813794.1	type II toxin-antitoxin system mRNA interferase toxin HicA	Toxin
HRR26_RS11225	2343836..2344066	+	231	WP_000494244.1	YncJ family protein	-
HRR26_RS11230	2344158..2346119	-	1962	WP_001303492.1	23S rRNA 5-hydroxycytidine C2501 synthase	-
HRR26_RS11235	2346192..2346728	-	537	WP_000429155.1	DNA-binding transcriptional regulator SutR	-
HRR26_RS11240	2346781..2347995	+	1215	WP_072003393.1	BenE family transporter YdcO	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	Similar MGE(s) Similar MGE(s) is defined as other TA-related MGE(s) with Mash distance < 0.01 to this MGE. Detail Genome accession Organism MGE type Related TA Coordinates Mash distance View NZ_CP020543 Escherichia coli C IS/Tn TA075073 2433298..2434446 0.008	flank	IS/Tn	-	-	2348035..2349183	1148

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-57)

Antitoxin

• Cro/C1-type helix-turn-helix domain (91-134)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6749.83 Da
• Isoelectric Point: 11.2298

>T33647 WP_000813794.1 NZ_AP021895:c2343614-2343438 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Nucleotide

• Length: 177 bp

>T33647 NZ_AP021895:c2343614-2343438 [Escherichia coli]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15246.63 Da
• Isoelectric Point: 4.7386

>AT33647 WP_001270286.1 NZ_AP021895:c2343392-2342976 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Nucleotide

• Length: 417 bp

>AT33647 NZ_AP021895:c2343392-2342976 [Escherichia coli]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
CCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCTTTACCTTCGCCATTAAATAGTCACGATCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAGGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAGGAGAT
TACTCGCCTATTTAACTTGCATCATGCGACAAAAATCGACGCCGTCCAGCTCGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA

Structures

Toxin

• PDB: 6HPB